課程資訊
課程名稱
微生物學實驗
Microbiology Lab 
開課學期
101-2 
授課對象
生化科技學系  
授課教師
張麗冠 
課號
BST2009 
課程識別碼
B02 20320 
班次
01 
學分
全/半年
半年 
必/選修
必帶 
上課時間
星期二5,6,7,8(12:20~16:20) 
上課地點
農化二B10-1 
備註
678堂於農化二108進行實驗。
限學號單號 且 限本系所學生(含輔系、雙修生)
總人數上限:35人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1012MicroLab_1 
課程簡介影片
 
核心能力關聯
本課程尚未建立核心能力關連
課程大綱
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課程概述

本課程主要是讓學生學習基本的微生物學操作技術,從顯微鏡的觀察開始,學習如何將細菌染色,觀察其形態構造,並利用生化特性鑑別細菌,以及分析微生物的遺傳特性,另外也將探討物理及化學因子對微生物生長之影響。 

課程目標
希望學生藉由此課程設計能引發對微生物學的興趣 
課程要求
 
預期每週課後學習時數
 
Office Hours
 
指定閱讀
 
參考書目
Cappuccino, JG, and Sherman, N (2011) Microbiology, A Laboratory Manual, 9ed. 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
02/19  課程介紹,實驗分組,實驗室安全守則,值日生分配 
第2週
02/26  停課 
第3週
03/05  Experiment 1: Culture transfer techniques
Experiment 2: Techniques for isolation of pure culture
Experiment 3: Cultural characteristics of microorganisms
 
第4週
03/12  Experiment 5: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount
Experiment 6: The microscopic measurement of microorganisms
 
第5週
03/19  Experiment 7: Preparation of bacterial smears
Experiment 8: Simple staining
Experiment 9: Negative staining
Experiment 10: Gram stain
 
第6週
03/26  Experiment 11: Acid-fast stain
Experiment 12: Differential staining for visualization of bacterial cell structure
Experiment: Flagella stain
 
第7週
04/2  溫書假停課 
第8週
04/09  Experiment 14: Use of differential and selective media Experiment 15: Physical factors: temperature Experiment 16: Physical factors: pH of the extracellular environment Experiment 17: Physical factors: atmosphere oxygen requirements 
第9週
04/16  Experiment 19: Serial dilution-agar plate procedure to quantitate viable cells
Experiment 20: The bacterial growth curve

Experiment 55: Isolation of a streptomycin-resistant mutant
Experiment 57: Bacterial transformation
Experiment: Bacterial conjugation and transposition
 
第10週
04/23  Experiment 38: Cultivation and enumeration of bacteriophages
Experiment 53: Enzyme induction
Experiment: Quorum sensing

 
第11週
04/30  Experiment 21: Extracellular enzymatic activities of microorganism
Experiment 22: Carbohydrate fermentation
Experiment 23: Triple sugar-iron agar test
Experiment 25: Hydrogen sulfide test
Experiment 26: Urease test
Experiment 27: Litmus milk reactions
 
第12週
05/07  Experiment 24: IMViC test
Experiment 28: Nitrate reduction test
Experiment 29: Catalase test
Experiment 30: Oxidase test
 
第13週
05/14  Experiment 40: Physical agents of control-moist heat Experiment 41: Physical agents of control-environmental osmotic pressure Experiment 42: Physical agents of control-electromagnetic radiations Experiment 43: Chemical agents of control-chemotherapeutic agents 
第14週
05/21  Experiment 44: Determination of penicillin activity in the presence and absence of penicillinase Experiment 45: Chemical agents of control-disinfectants and antiseptics Experiment 48: Standard qualitative analysis of water Experiment 56: The Ames test: A bacterial test system for chemical carcinogenicity 
第15週
05/28  參觀台碩 台美生技公司
 
第16週
06/04  期末考
Experiment 32: Genus identification of unknown bacterial cultures