|
週次 |
日期 |
單元主題 |
|
第1週 |
2/24 |
課程介紹與分組，實驗室安全守則，值日生分配 |
|
第2週 |
3/03 |
準備周 |
|
第3週 |
3/10 |
Experiment 1: Effectiveness of hand washing</br>
Experiment 2: Culture transfer techniques</br>
Experiment 3: Techniques for isolation of pure culture</br>
Experiment 4: Cultural characteristics of microorganisms
|
|
第4週 |
3/17 |
Experiment 5, 6: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount</br>
Experiment 7: The microscopic measurement of microorganisms
|
|
第5週 |
3/24 |
Experiment 8: Preparation of bacterial smears</br>
Experiment 9: Simple staining</br>
Experiment 10: Negative staining</br>
Experiment 11: Gram stain
|
|
第6週 |
3/31 |
溫書假停課 |
|
第7週 |
4/07 |
Experiment 12: Acid-fast stain</br>
Experiment 13: Differential staining for visualization of bacterial cell structure</br>
Experiment: Flagella stain
|
|
第8週 |
4/14 |
Expriment 15: Use of differential and selective media</br>
Experiment 16 Physical factors: temperature</br>
Experiment 17: Physical factors: pH of the extracellular environment</br>
Experiment 18: Physical factors: atmosphere oxygen requirements
|
|
第9週 |
4/21 |
Experiment 20: Serial dilution-agar plate procedure to quantitate viable cells</br>
Experiment 21: The bacterial growth curve</br>
Experiment 56: Isolation of a streptomycin-resistant mutant</br>
Experiment 58: Bacterial transformation
|
|
第10週 |
4/28 |
Experiment 55: Bacterial conjugation and transposition</br>
Experiment 39: Cultivation and enumeration of bacteriophages</br>
Experiment 54: Enzyme induction
|
|
第11週 |
5/05 |
Experiment 22: Extracellular enzymatic activities of microorganism</br>
Experiment 23: Carbohydrate fermentation</br>
Experiment 24: Triple sugar-iron agar test</br>
Experiment 26: Hydrogen sulfide test</br>
Experiment 27: Urease test</br>
Experiment 28: Litmus milk reactions
|
|
第12週 |
5/12 |
Experiment 25: IMViC test</br>
Experiment 29: Nitrate reduction test</br>
Experiment 30: Catalase test</br>
Experiment 31: Oxidase test
|
|
第13週 |
5/19 |
Experiment 41: Physical agents of control-moist heat</br>
Experiment 42: Physical agents of control-environmental osmotic pressure</br>
Experiment 43: Physical agents of control-electromagnetic radiations</br>
Experiment 44: Chemical agents of control-chemotherapeutic agents
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|
第14週 |
5/26 |
Experiment 45: Determination of penicillin activity in the presence and absence of penicillinase</br>
Experiment 46: Chemical agents of control-disinfectants and antiseptics
|
|
第15週 |
6/02 |
Experiment 49: Standard qualitative analysis of water</br>
Experiment 57: The Ames test: A bacterial test system for chemical carcinogenicity
|
|
第16週 |
6/09 |
期末考</br>
Experiment 33: Genus identification of unknown bacterial cultures</br>
綜合討論，教學意見調查， 實驗室整理
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