|
週次 |
日期 |
單元主題 |
|
第1週 |
2/21 |
課程介紹與分組，實驗室安全守則，值日生分配 |
|
第2週 |
2/28 |
二二八和平紀念日，停課一次 |
|
第3週 |
3/07 |
Microscopy
Experiment 5, 6: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount
Experiment 7: The microscopic measurement of microorganisms
|
|
第4週 |
3/14 |
Basic Laboratory Techniques
Experiment 2: Culture transfer techniques
Experiment 3: Techniques for isolation of pure cultures
Experiment 4: Cultural characteristics of microorganisms
|
|
第5週 |
3/21 |
Bacterial Staining-I
Experiment 8: Preparation of bacterial smears
Experiment 9: Simple staining
Experiment 10: Negative staining
Experiment 11: Gram stain
|
|
第6週 |
3/28 |
Bacterial Staining-II
Experiment 12: Acid-fast stain
Experiment 13: Differential staining for visualization of bacterial cell structure
Experiment: Flagella stain
|
|
第7週 |
4/04 |
清明節，停課一次 |
|
第8週 |
4/11 |
Experiment 20: Serial dilution-agar plate procedure to
quantitate viable cells
Experiment 21: The bacterial growth curve
Bacterial Genetics-I
Experiment 56: Isolation of a streptomycin-resistant
mutant
Experiment 58: Bacterial transformation
|
|
第9週 |
4/18 |
Expriment 15: Use of differential and selective media
Experiment 16: Physical factors: temperature
Experiment 17: Physical factors: pH of the extracellular environment
Experiment 18: Physical factors: atmosphere oxygen
Requirements
|
|
第10週 |
4/25 |
Bacterial Genetics-II
Experiment 55: Bacterial conjugation and transposition
Experiment 39: Cultivation and enumeration of bacteriophages
Experiment 54: Enzyme induction |
|
第11週 |
5/02 |
Biochemical Activities of Microorganisms-I
Experiment 22: Extracellular enzymatic activities of microorganism
Experiment 23: Carbohydrate fermentation
Experiment 24: Triple sugar-iron agar test
|
|
第12週 |
5/09 |
Biochemical Activities of Microorganisms-II
Experiment 25: IMViC test
Experiment 26: Hydrogen sulfide test
Experiment 27: Urease test
Experiment 28: Litmus milk reactions
Experiment 29: Nitrate reduction test
Experiment 30: Catalase test
Experiment 31: Oxidase test |
|
第13週 |
5/16 |
Physical and Chemical Agents for the Control of Microbial Growth-I
Experiment 41: Physical agents of control-moist heat
Experiment 42: Physical agents of control-environmental osmotic pressure
Experiment 43: Physical agents of control-electromagnetic radiations
Experiment 44: Chemical agents of control-chemotherapeutic agents
|
|
第14週 |
5/23 |
Physical and Chemical Agents for the Control of Microbial Growth-II
Experiment 45: Determination of penicillin activity in the presence and absence of penicillinase
Experiment 46: Chemical agents of control-disinfectants and antiseptics
|
|
第15週 |
5/30 |
端午節，停課一次 |
|
第16週 |
6/06 |
Experiment 49: Standard qualitative analysis of waterExperiment 57: The Ames test: A bacterial test system for chemical carcinogenicity |
|
第17週 |
6/13 |
期末考
Experiment 33: Genus identification of unknown bacterial cultures
綜合討論，教學意見調查， 實驗室整理
|