週次 |
日期 |
單元主題 |
第1週 |
2/23 |
課程介紹與分組,實驗室安全守則,值日生分配
|
第2週 |
3/02 |
Basic Laboratory Techniques
Experiment 1: Culture transfer techniques
Experiment 2: Techniques for isolation of pure cultures
Experiment 3: Cultural characteristics of microorganisms
|
第3週 |
0309 |
Microscopy
Experiment 4: Microscopic examination of stained cell preparations
Experiment 5: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount |
第4週 |
3/16 |
Bacterial Staining-I
Experiment 6: Preparation of bacterial smears
Experiment 7: Simple staining
Experiment 8: Negative staining
Experiment 9: Gram stain |
第5週 |
3/23 |
Bacterial Staining-II
Experiment 10: Acid-fast stain
Experiment 11: Differential staining for visualization of bacterial cell structure
Experiment: Flagella stain |
第8週 |
4/13 |
Experiment 13: Use of differential and selective mediaExperiment 14: Physical factors: temperatureExperiment 15: Physical factors: pH of the extracellular environmentExperiment 16: Physical factors: atmosphere oxygen requirements |
第9週 |
4/20 |
Experiment 18: Serial dilution-agar plate procedure to quantitate viable cells
Experiment 19: The bacterial growth curve
Bacterial Genetics-I
Experiment 54: Isolation of a streptomycin-resistant mutant
Experiment 56: Bacterial transformation |
第10週 |
4/27 |
Bacterial Genetics-II
Experiment: Bacterial conjugation and transposition
Experiment 52: Enzyme induction
Experiment 55: The Ames test: A bacterial test system for chemical carcinogenicity |
第11週 |
5/04 |
Biochemical Activities of Microorganisms-I
Experiment 20: Extracellular enzymatic activities of microorganism
Experiment 21: Carbohydrate fermentation
Experiment 22: Triple sugar-iron agar test
Experiment 24: Hydrogen sulfide test |
第12週 |
5/11 |
Biochemical Activities of Microorganisms-II
Experiment 23: IMViC test
Experiment 25: Urease test
Experiment 26: Litmus milk reactions
Experiment 27: Nitrate reduction test
Experiment 28: Catalase test
Experiment 29: Oxidase test |
第13週 |
5/18 |
Experiment 37: Cultivation and enumeration of bacteriophages
Experiment 47: Standard qualitative analysis of water |
第14週 |
5/25 |
Physical and Chemical Agents for the Control of Microbial Growth-I
Experiment 40: Physical agents of control-moist heat
Experiment 41: Physical agents of control-electromagnetic radiations |
第15週 |
6/01 |
Physical and Chemical Agents for the Control of Microbial Growth-II
Experiment 42: Chemical agents of control-chemotherapeutic agents
Experiment 43: Determination of penicillin activity in the presence and absence of penicillinase |
第16週 |
6/08 |
期末考
Experiment 31: Genus identification of unknown bacterial cultures
綜合討論,教學意見調查, 實驗室整理 |