週次 |
日期 |
單元主題 |
第1週 |
2/21 |
課程介紹,實驗分組,實驗室安全守則,值日生分配 |
第2週 |
2/28 |
停課 |
第3週 |
3/07 |
Experiment 1: Culture transfer techniques
Experiment 2: Techniques for isolation of pure culture
Experiment 3: Cultural characteristics of microorganisms
|
第4週 |
3/14 |
Experiment 5: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount
Experiment 6: The microscopic measurement of microorganisms
|
第5週 |
3/21 |
Experiment 7: Preparation of bacterial smears
Experiment 8: Simple staining
Experiment 9: Negative staining
Experiment 10: Gram stain
|
第6週 |
3/28 |
Experiment 11: Acid-fast stain
Experiment 12: Differential staining for visualization of bacterial cell structure
Experiment: Flagella stain
|
第7週 |
4/04 |
溫書假停課 |
第8週 |
4/11 |
Experiment 14: Use of differential and selective media
Experiment 15: Physical factors: temperature
Experiment 16: Physical factors: pH of the extracellular environment
Experiment 17: Physical factors: atmosphere oxygen requirements
|
第9週 |
4/18 |
Experiment 19: Serial dilution-agar plate procedure to quantitate viable cells
Experiment 20: The bacterial growth curve
Experiment 55: Isolation of a streptomycin-resistant mutant
Experiment 57: Bacterial transformation
Experiment: Bacterial conjugation and transposition
|
第10週 |
4/25 |
Experiment 38: Cultivation and enumeration of bacteriophages
Experiment 53: Enzyme induction
Experiment: Quorum sensing
|
第11週 |
5/02 |
Experiment 21: Extracellular enzymatic activities of microorganism
Experiment 22: Carbohydrate fermentation
Experiment 23: Triple sugar-iron agar test
Experiment 25: Hydrogen sulfide test
Experiment 26: Urease test
Experiment 27: Litmus milk reactions
|
第12週 |
5/09 |
Experiment 24: IMViC test
Experiment 28: Nitrate reduction test
Experiment 29: Catalase test
Experiment 30: Oxidase test
|
第13週 |
5/16 |
Experiment 40: Physical agents of control-moist heat Experiment 41: Physical agents of control-environmental osmotic pressure Experiment 42: Physical agents of control-electromagnetic radiations Experiment 43: Chemical agents of control-chemotherapeutic agents |
第14週 |
5/23 |
Experiment 44: Determination of penicillin activity in the presence and absence of penicillinase Experiment 45: Chemical agents of control-disinfectants and antiseptics Experiment 48: Standard qualitative analysis of water Experiment 56: The Ames test: A bacterial test system for chemical carcinogenicity |
第15週 |
5/30 |
參觀台碩 臺美生技公司 |
第16週 |
6/06 |
期末考
Experiment 32: Genus identification of unknown bacterial cultures
|