課程資訊
課程名稱
微生物學實驗
Microbiology Lab 
開課學期
102-2 
授課對象
生化科技學系  
授課教師
張麗冠 
課號
BST2009 
課程識別碼
B02 20320 
班次
02 
學分
全/半年
半年 
必/選修
必帶 
上課時間
星期四5,6,7,8(12:20~16:20) 
上課地點
農化一第六 
備註
678堂於農化二108進行實驗。
限學號雙號 且 限本系所學生(含輔系、雙修生)
總人數上限:35人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1022BST2009_02 
課程簡介影片
 
核心能力關聯
本課程尚未建立核心能力關連
課程大綱
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課程概述

本課程主要是讓學生學習基本的微生物學操作技術,從顯微鏡的觀察開始,學習如何將細菌染色,觀察其形態構造,並利用生化特性鑑別細菌,以及分析微生物的遺傳特性,另外也將探討物理及化學因子對微生物生長之影響。 

課程目標
希望學生藉由此課程設計能引發對微生物學的興趣 
課程要求
待補 
預期每週課後學習時數
 
Office Hours
 
指定閱讀
待補 
參考書目
Cappuccino, JG, and Sherman, N (2011) Microbiology, A Laboratory Manual, 9ed. 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
2/20  課程介紹,實驗分組,實驗室安全守則,值日生分配 
第2週
2/27  Experiment 5, 6: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount <br/>
Experiment 7: The microscopic measurement of microorganisms 
第3週
3/06  Experiment 1: Effectiveness of hand washing
<br/>
Experiment 2: Culture transfer techniques <br/>
Experiment 3: Techniques for isolation of pure culture <br/>
Experiment 4: Cultural characteristics of microorganisms
 
第4週
3/13  Experiment 8: Preparation of bacterial smears<br/>
Experiment 9: Simple staining<br/>
Experiment 10: Negative staining<br/>
Experiment 11: Gram stain
 
第5週
3/20  Experiment 12: Acid-fast stain <br/>
Experiment 13: Differential staining for visualization of bacterial cell structure <br/>
Experiment: Flagella stain
 
第6週
3/27  Expriment 15: Use of differential and selective media <br/>
Experiment 16 Physical factors: temperature<br/>
Experiment 17: Physical factors: pH of the extracellular environment<br/>
Experiment 18: Physical factors: atmosphere oxygen requirements
 
第7週
4/03  溫書假停課 
第8週
4/10  Experiment 20: Serial dilution-agar plate procedure to quantitate viable cells<br/>
Experiment 21: The bacterial growth curve<br/>
Experiment 56: Isolation of a streptomycin-resistant mutant<br/>
Experiment 58: Bacterial transformation
 
第9週
4/17  期中考停課 
第10週
4/24  Experiment 55: Bacterial conjugation and transposition <br/>
Experiment 39: Cultivation and enumeration of bacteriophages<br/>
Experiment 54: Enzyme induction<br/>
Experiment: Quorum sensing
 
第11週
5/01  Experiment 22: Extracellular enzymatic activities of microorganism <br/>
Experiment 23: Carbohydrate fermentation<br/>
Experiment 24: Triple sugar-iron agar test<br/>
Experiment 26: Hydrogen sulfide test<br/>
Experiment 27: Urease test<br/>
Experiment 28: Litmus milk reactions
 
第12週
5/08  Experiment 25: IMViC test <br/>
Experiment 29: Nitrate reduction test<br/>
Experiment 30: Catalase test<br/>
Experiment 31: Oxidase test
 
第13週
5/15  Experiment 41: Physical agents of control-moist heat <br/>
Experiment 42: Physical agents of control-environmental osmotic pressure<br/>
Experiment 43: Physical agents of control-electromagnetic radiations<br/>
Experiment 44: Chemical agents of control-chemotherapeutic agents
 
第14週
5/22  Experiment 45: Determination of penicillin activity in the presence and absence of penicillinase <br/>
Experiment 46: Chemical agents of control-disinfectants and antiseptics
 
第15週
5/29  Experiment 45: Determination of penicillin activity in the presence and absence of penicillinase <br/>
Experiment 46: Chemical agents of control-disinfectants and antiseptics
 
第16週
06/03  期末考 <br/>
Experiment 33: Genus identification of unknown bacterial cultures <br/>
綜合討論,教學意見調查, 實驗室整理