週次 |
日期 |
單元主題 |
第1週 |
2/20 |
課程介紹,實驗分組,實驗室安全守則,值日生分配 |
第2週 |
2/27 |
Experiment 5, 6: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount <br/>
Experiment 7: The microscopic measurement of microorganisms |
第3週 |
3/06 |
Experiment 1: Effectiveness of hand washing
<br/>
Experiment 2: Culture transfer techniques <br/>
Experiment 3: Techniques for isolation of pure culture <br/>
Experiment 4: Cultural characteristics of microorganisms
|
第4週 |
3/13 |
Experiment 8: Preparation of bacterial smears<br/>
Experiment 9: Simple staining<br/>
Experiment 10: Negative staining<br/>
Experiment 11: Gram stain
|
第5週 |
3/20 |
Experiment 12: Acid-fast stain <br/>
Experiment 13: Differential staining for visualization of bacterial cell structure <br/>
Experiment: Flagella stain
|
第6週 |
3/27 |
Expriment 15: Use of differential and selective media <br/>
Experiment 16 Physical factors: temperature<br/>
Experiment 17: Physical factors: pH of the extracellular environment<br/>
Experiment 18: Physical factors: atmosphere oxygen requirements
|
第7週 |
4/03 |
溫書假停課 |
第8週 |
4/10 |
Experiment 20: Serial dilution-agar plate procedure to quantitate viable cells<br/>
Experiment 21: The bacterial growth curve<br/>
Experiment 56: Isolation of a streptomycin-resistant mutant<br/>
Experiment 58: Bacterial transformation
|
第9週 |
4/17 |
期中考停課 |
第10週 |
4/24 |
Experiment 55: Bacterial conjugation and transposition <br/>
Experiment 39: Cultivation and enumeration of bacteriophages<br/>
Experiment 54: Enzyme induction<br/>
Experiment: Quorum sensing
|
第11週 |
5/01 |
Experiment 22: Extracellular enzymatic activities of microorganism <br/>
Experiment 23: Carbohydrate fermentation<br/>
Experiment 24: Triple sugar-iron agar test<br/>
Experiment 26: Hydrogen sulfide test<br/>
Experiment 27: Urease test<br/>
Experiment 28: Litmus milk reactions
|
第12週 |
5/08 |
Experiment 25: IMViC test <br/>
Experiment 29: Nitrate reduction test<br/>
Experiment 30: Catalase test<br/>
Experiment 31: Oxidase test
|
第13週 |
5/15 |
Experiment 41: Physical agents of control-moist heat <br/>
Experiment 42: Physical agents of control-environmental osmotic pressure<br/>
Experiment 43: Physical agents of control-electromagnetic radiations<br/>
Experiment 44: Chemical agents of control-chemotherapeutic agents
|
第14週 |
5/22 |
Experiment 45: Determination of penicillin activity in the presence and absence of penicillinase <br/>
Experiment 46: Chemical agents of control-disinfectants and antiseptics
|
第15週 |
5/29 |
Experiment 45: Determination of penicillin activity in the presence and absence of penicillinase <br/>
Experiment 46: Chemical agents of control-disinfectants and antiseptics
|
第16週 |
06/03 |
期末考 <br/>
Experiment 33: Genus identification of unknown bacterial cultures <br/>
綜合討論,教學意見調查, 實驗室整理
|