週次 |
日期 |
單元主題 |
第1週 |
2/26 |
課程介紹與分組,實驗室安全守則,值日生分配 |
第2週 |
3/05 |
準備周 |
第3週 |
3/12 |
Experiment 1: Effectiveness of hand washing </br>
Experiment 2: Culture transfer techniques</br>
Experiment 3: Techniques for isolation of pure culture</br>
Experiment 4: Cultural characteristics of microorganisms
|
第4週 |
3/19 |
Experiment 5, 6: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount</br>
Experiment 7: The microscopic measurement of microorganisms
|
第5週 |
3/26 |
Experiment 8: Preparation of bacterial smears</br>
Experiment 9: Simple staining</br>
Experiment 10: Negative staining</br>
Experiment 11: Gram stain
|
第6週 |
4/02 |
溫書假停課 |
第7週 |
4/09 |
Experiment 12: Acid-fast stain</br>
Experiment 13: Differential staining for visualization of bacterial cell structure</br>
Experiment: Flagella stain
|
第8週 |
4/16 |
Expriment 15: Use of differential and selective media</br>
Experiment 16 Physical factors: temperature</br>
Experiment 17: Physical factors: pH of the extracellular environment</br>
Experiment 18: Physical factors: atmosphere oxygen requirements
|
第9週 |
4/23 |
Experiment 20: Serial dilution-agar plate procedure to quantitate viable cells</br>
Experiment 21: The bacterial growth curve</br>
Experiment 56: Isolation of a streptomycin-resistant mutant</br>
Experiment 58: Bacterial transformation
|
第10週 |
4/30 |
Experiment 55: Bacterial conjugation and transposition</br>
Experiment 39: Cultivation and enumeration of bacteriophages</br>
Experiment 54: Enzyme induction
|
第11週 |
5/07 |
Experiment 22: Extracellular enzymatic activities of microorganism</br>
Experiment 23: Carbohydrate fermentation</br>
Experiment 24: Triple sugar-iron agar test</br>
Experiment 26: Hydrogen sulfide test</br>
Experiment 27: Urease test</br>
Experiment 28: Litmus milk reactions
|
第12週 |
5/14 |
Experiment 25: IMViC test</br>
Experiment 29: Nitrate reduction test</br>
Experiment 30: Catalase test</br>
Experiment 31: Oxidase test
|
第13週 |
5/21 |
Experiment 41: Physical agents of control-moist heat</br>
Experiment 42: Physical agents of control-environmental osmotic pressure</br>
Experiment 43: Physical agents of control-electromagnetic radiations</br>
Experiment 44: Chemical agents of control-chemotherapeutic agents
|
第14週 |
5/28 |
Experiment 45: Determination of penicillin activity in the presence and absence of penicillinase</br>
Experiment 46: Chemical agents of control-disinfectants and antiseptics
|
第15週 |
6/04 |
Experiment 49: Standard qualitative analysis of water</br>
Experiment 57: The Ames test: A bacterial test system for chemical carcinogenicity
|
第16週 |
6/11 |
期末考</br>
Experiment 33: Genus identification of unknown bacterial cultures</br>
綜合討論,教學意見調查, 實驗室整理
|