課程資訊
課程名稱
微生物學實驗
Microbiology Lab 
開課學期
104-2 
授課對象
生化科技學系  
授課教師
張麗冠 
課號
BST2009 
課程識別碼
B02 20320 
班次
02 
學分
全/半年
半年 
必/選修
必帶 
上課時間
星期四6,7,8,9(13:20~17:20) 
上課地點
農化一第六 
備註
789節於農化二108進行實驗。與林晉玄合開
限學號雙號 且 限本系所學生(含輔系、雙修生)
總人數上限:35人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1042BST2009_02Mlab_2 
課程簡介影片
 
核心能力關聯
本課程尚未建立核心能力關連
課程大綱
為確保您我的權利,請尊重智慧財產權及不得非法影印
課程概述

本課程主要是讓學生學習基本的微生物學操作技術,從顯微鏡的觀察開始,學習如何將細菌染色,觀察其形態構造,並利用生化特性鑑別細菌,以及分析微生物的遺傳特性,另外也將探討物理及化學因子對微生物生長之影響。 

課程目標
希望學生藉由此課程設計能引發對微生物學的興趣 
課程要求
待補 
預期每週課後學習時數
 
Office Hours
 
指定閱讀
待補 
參考書目
Cappuccino, JG, and Sherman, N (2014) Microbiology, A Laboratory Manual, 10ed. 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
2/25  課程介紹與分組 
第2週
3/03  Microscopy
Experiment 5, 6: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount
Experiment 7: The microscopic measurement of microorganisms
 
第3週
3/10  Basic Laboratory Techniques
Experiment 2: Culture transfer techniques
Experiment 3: Techniques for isolation of pure cultures
Experiment 4: Cultural characteristics of microorganisms
 
第4週
3/17  Bacterial Staining-I
Experiment 8: Preparation of bacterial smears
Experiment 9: Simple staining
Experiment 10: Negative staining
Experiment 11: Gram stain
 
第5週
3/24  Bacterial Staining-II
Experiment 12: Acid-fast stain
Experiment 13: Differential staining for visualization of bacterial cell structure
Experiment: Flagella stain
 
第6週
3/31  Expriment 15: Use of differential and selective media
Experiment 16: Physical factors: temperature
Experiment 17: Physical factors: pH of the extracellular environment
Experiment 18: Physical factors: atmosphere oxygen
requirements
 
第7週
4/07  清明節,停課乙次 
第8週
4/14  Experiment 20: Serial dilution-agar plate procedure to quantitate viable cells
Experiment 21: The bacterial growth curve

Bacterial Genetics-I
Experiment 56: Isolation of a streptomycin-resistant mutant
Experiment 58: Bacterial transformation
 
第9週
4/21  期中考週,溫書假 
第10週
4/28  Bacterial Genetics-II
Experiment 55: Bacterial conjugation and transposition
Experiment 39: Cultivation and enumeration of bacteriophages
Experiment 54: Enzyme induction
 
第11週
5/05  Biochemical Activities of Microorganisms-I
Experiment 22: Extracellular enzymatic activities of microorganism
Experiment 23: Carbohydrate fermentation
Experiment 24: Triple sugar-iron agar test
Experiment 26: Hydrogen sulfide test
Experiment 27: Urease test
Experiment 28: Litmus milk reactions
 
第12週
5/12  Biochemical Activities of Microorganisms-II
Experiment 25: IMViC test
Experiment 29: Nitrate reduction test
Experiment 30: Catalase test
Experiment 31: Oxidase test
 
第13週
5/19  Physical and Chemical Agents for the Control of Microbial Growth-I
Experiment 41: Physical agents of control-moist heat
Experiment 42: Physical agents of control-environmental osmotic pressure
Experiment 43: Physical agents of control-electromagnetic radiations
Experiment 44: Chemical agents of control-chemotherapeutic agents
 
第14週
5/26  Physical and Chemical Agents for the Control of Microbial Growth-II
Experiment 45: Determination of penicillin activity in the presence and absence of penicillinase
Experiment 46: Chemical agents of control-disinfectants and antiseptics
 
第15週
6/02  Experiment 49: Standard qualitative analysis of water
Experiment 57: The Ames test: A bacterial test system for chemical carcinogenicity
 
第16週
6/09  端午節,停課乙次 
第17週
6/16  期末考
Experiment 33: Genus identification of unknown bacterial cultures