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課程名稱 |
微生物學實驗
Microbiology Lab |
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開課學期 |
99-2 |
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授課對象 |
生化科技學系 |
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授課教師 |
張麗冠 |
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課號 |
BST2009 |
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課程識別碼 |
B02 20320 |
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班次 |
02 |
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學分 |
2 |
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全/半年 |
半年 |
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必/選修 |
必帶 |
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上課時間 |
星期四5,6,7,8(12:20~16:20) |
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上課地點 |
農化一第六 |
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備註 |
678堂於農化二108進行實驗。
限學號雙號 且 限本系所學生(含輔系、雙修生)
總人數上限：35人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/992microlab2 |
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課程簡介影片 |
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核心能力關聯 |
本課程尚未建立核心能力關連 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
本課程主要是讓學生學習基本的微生物學操作技術，從顯微鏡的觀察開始，學習如何將細菌染色，觀察其形態構造，並利用生化特性鑑別細菌，以及分析微生物的遺傳特性，另外也將探討物理及化學因子對微生物生長之影響。 |
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課程目標 |
希望學生藉由此課程設計能引發對微生物學的興趣 |
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課程要求 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
Cappuccino, JG, and Sherman, N (2008) Microbiology, A Laboratory Manual, 8ed. |
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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第1週 |
02/24 |
課程介紹，實驗分組，實驗室安全守則，值日生分配 |
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第2週 |
03/03 |
Experiment 1: Culture transfer techniques,
Experiment 2: Techniques for isolation of pure culture,
Experiment 3: Cultural characteristics of microorganisms
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第3週 |
03/10 |
Experiment 5: Microscopic examination of living microorganisms using a hanging-drop preparation or a wet mount,
Experiment 6: The microscopic measurement of microorganisms
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第4週 |
03/17 |
Experiment 7: Preparation of bacterial smears,
Experiment 8: Simple staining,
Experiment 9: Negative staining,
Experiment 10: Gram stain
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第5週 |
03/24 |
Experiment 11: Acid-fast stain,
Experiment 12: Differential staining for visualization of bacterial cell structure,
Experiment: Flagella stain
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第6週 |
03/31 |
Experiment 14: Use of differential and selective media
Experiment 15: Physical factors: temperature,
Experiment 16: Physical factors: pH of the extracellular environment,
Experiment 17: Physical factors: atmosphere oxygen requirements
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第7週 |
04/07 |
溫書假 |
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第8週 |
04/14 |
期中考停課 |
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第9週 |
04/21 |
Experiment 35: Cultivation and morphology of Molds,
Experiment 19: Serial dilution-agar plate procedure to quantitate viable cells,
Experiment 20: The bacterial growth curve
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第10週 |
04/28 |
Experiment 55: Isolation of a streptomycin-resistant mutant,
Experiment 57: Bacterial transformation,
Experiment: Bacterial conjugation and transposition
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第11週 |
05/05 |
Experiment 38: Cultivation and enumeration of bacteriophages,
Experiment 53: Enzyme induction,
Experiment: Quorum sensing |
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第12週 |
05/12 |
Experiment 21: Extracellular enzymatic activities of microorganism,
Experiment 22: Carbohydrate fermentation,
Experiment 23: Triple sugar-iron agar test,
Experiment 25: Hydrogen sulfide test,
Experiment 26: Urease test,
Experiment 27: Litmus milk reactions
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第13週 |
05/19 |
Experiment 24: IMViC test,
Experiment 28: Nitrate reduction test,
Experiment 29: Catalase test,
Experiment 30: Oxidase test,
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第14週 |
05/26 |
Experiment 40: Physical agents of control-moist heat,
Experiment 41: Physical agents of control-environmental osmotic pressure,
Experiment 42: Physical agents of control-electromagnetic radiations,
Experiment 43: Chemical agents of control-chemotherapeutic agents
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第15週 |
06/02 |
Experiment 44: Determination of penicillin activity in the presence and absence of penicillinase,
Experiment 45: Chemical agents of control-disinfectants and antiseptics
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第16週 |
06/09 |
Experiment 48: Standard qualitative analysis of water,
Experiment 56: The Ames test: A bacterial test system for chemical carcinogenicity
期末考(Experiment 32: Genus identification of unknown bacterial cultures) |
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第17週 |
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綜合討論，教學意見調查， 實驗室整理 |
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