課程名稱 |
分析化學乙下 Analytical Chemistry (b)(2) |
開課學期 |
101-2 |
授課對象 |
生物資源暨農學院 森林環境暨資源學系 |
授課教師 |
張哲政 |
課號 |
Chem2012 |
課程識別碼 |
203 211B2 |
班次 |
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學分 |
2 |
全/半年 |
全年 |
必/選修 |
必修 |
上課時間 |
星期五3,4(10:20~12:10) |
上課地點 |
新102 |
備註 |
生物材料群選必。詳化學系選課須知。請依指定修習院系班次修習。先修:普化。上下學期需選同一班次。 總人數上限:120人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1012afc_2 |
課程簡介影片 |
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核心能力關聯 |
本課程尚未建立核心能力關連 |
課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
This course extends the knowledge of the analytical chemistry to include the fundamental principles and application of modern instrumental methods including spectroscopic, spectrometric, and separation methods. The topics covered have applications to many subject areas in Natural Sciences, Life Science and Engineering.
This course is presented in a lecture/discussion format. The content of the course includes:
Part I:
I. Electrolysis
A. Electrogravimetry
a) Analysis and Electrode
b) Separation
c) Non-potentiostatic Method
i) Concentration Polarization
ii) Kinetic Polarization
ii) Overpotential
iv) Ecat Adjustment
v) I-V Behavior During Electrolysis
d) Potentiostatic Method
i) Three-Electrode System
ii) Potential and Current Changes
B. Coulometry
a) Analysis
II. Amperometry
A. Analysis
B. O2 Measurement
C. Glucose Electrode
D. Blood Glucose Monitor
E. Chromatographic Detector
III. Voltammetry
A. Applied Potential, Eappl
a) Linear Sweep
b) Staircase
c) Square Wave
B. Current, i
C. Basis of Analysis
a) Faradaic and Charging Currents
b) Supporting Electrolyte
c) Vitamin C Analysis
D. Cyclic Voltammetry
E. Sampled Current Polarography
F. Square Wave Voltammetry
G. Stripping Method
Part II:
I. Introduction to Instrumental Analysis
II. Fundamentals of Spectroscopy/Spectrometry
A. Introduction to Spectroscopy
B. Characteristics of Electromagnetic radiation
C. Wave Property of Light and Optical Component
a) Interference
b) Transmission
c) Reflection
d) Refraction
e) Scattering
f) Diffraction
D. Particle Property of Light and Its Use
a) Photoelectric effect
b) Absorption
c) Emission
E. Spectroscopy
a) Type of Spectroscopy
b) Nature of Excitation
III. UV and Visible Absorption Spectrometry
A. Range and Type of UV/Vis Radiation
B. Principle
a) Molar Absorptivity
b) Spectroscopic Process
c) Effect of Multichromophores
d) d, f-electron Transition
C. Measurement
a) Standard Addition Method
b) Solvent Selection
c) Solvent Effect
d) Beer’s Law and Its Limitations
e) Spectrophotometric Uncertainty
D. Instrumentation
a) Single-Beam and Double-Beam Instruments
b) Sample Cell and Construction Materials
c) Source
d) Slit
e) Detector
E. Spectroscopic Information
a) Imaging
b) Qualitative
c) Quantitative
F. Spectral Analysis
a) Woodward-Fieser Rules
IV. Luminescence Spectroscopy
A. Luminescence
B. Principle
a) Spectroscopic Process
b) Quantum Yield
c) Favorable Condition for Luminescence
d) Emission Spectrum
e) 90˚ Detection
f) Luminescence Intensity
g) Type of Luminescence
C. Instrumentation
D. Spectroscopic Information
a) FL System
b) Fluorescent Agent
c) Use of Excited-State Molecules
d) Fluorescence Resonance Energy Transfer
V. Infrared Spectroscopy
A. Range and Type of IR Radiation
B. Principle
a) Molecular Excitation
b) Type of Molecular Vibration
c) Normal Mode of Vibration
d) Spectroscopic Process
e) IR-Active Vibration
C. Instrumentation
a) Nondispersive Instrument
b) Dispersive Instrument
c) Sample Cell and Construction Materials
d) Source
e) Wavelength Selector
f) Detector
D. Spectroscopic Information
a) Functional Group
b) Quantitative
VI. Raman Spectroscopy
A. Principle
a) Polarizability
b) Raman Scattering
c) Energy Shift
d) Raman-Active Vibration
e) Raman Scattering Intensity
f) Advantage Over IR
B. Instrumentation
a) Laser
C. Spectroscopic Information
D. Raman vs. IR
VII. Atomic Spectroscopy
A. Spectroscopic Process
a) Absorption
b) Emission
B. Atomization
a) Flame
b) Furnace
c) Plasma
C. Spectral Line Shape
a) Natural Line Width
i) Heisenberg Uncertainty Principle
ii) Full Width at Half Maximum
b) Pressure Broadening
c) Doppler Broadening
d) Background Radiation
e) Molecular Emission
f) Self-Absorption
D. Spectrometer Design
a) Main Components
b) Component Layout
c) Sources
VIII. Nuclear Magnetic Resonance Spectroscopy
A. Principle
a) Range of Radiation
b) Nuclear Spin
B. Instrumentation and Measurement
a) Phase Coherence
b) Magnetic Resonance
c) Secondary Field
IX. Mass Spectrometry
A. Ionization Method
a) Gas-Phase Ionization
i) Electron Impact
ii) Chemical Ionization
iii) Field Ionization
b) Liquid-Phase Ionization
i) Inductively Coupled Plasma
ii) Electrospray Ionization
c) Solid-Phase Ionization
i) Fast Atom Bombardment
ii) Matrix-Assisted Laser Desorption/Ionization
B. Mass Analyzer
a) Double Focusing
b) Time-of-Flight
c) Quadrupole
d) Ion Trap
C. Detector
a) Faraday Cup
b) Channel Electron Multiplier
c) Multichannel Plate
D. Spectral Analysis
X. Analytical Separation
A. General Description
a) Solvent Extraction
b) Mobile and Stationary Phases
c) Classification
d) Elution
B. Principle
a) Migration Rate
b) Retention Time
c) Flow Rate – Flow Velocity Relationship
d) Migration Rate - Distribution Constant
e) Retention Factor
f) Selectivity Factor
g) Band Broadening
h) Column Efficiency
i) Column Resolution
j) Column Resolution
XI. Gas Chromatography
A. Separation Process
B. Injection and Detection
C. Detector
D. Sample Preparation
XII. High-Performance Liquid Chromatography
A. Chromatographic Process
B. Injection and Detection
C. Reversed-Phase Separation
D. Gradient Separation |
課程目標 |
This course provides students with the background theory and operation principles of chemical analysis methods. The specific objectives of the course are as follows:
◎to become familiar with the various spectroscopy, spectrometry, and separation methods currently available
◎to develop an understanding of the theories upon which the principles of the common spectroscopic, spectrometric, and separation procedures are based
◎to understand the correlations of the spectroscopic/spectrometric/separation analysis concepts and of various controllable and measurable quantities
to learn how the different spectroscopic/spectrometric/separation analysis methods are used to analyze samples
◎to understand the limitations and strengths of particular analysis approaches
◎to gain skill and competence in the use of related analytical standards/references
◎to learn to apply chemical equilibria and stoichiometry to solve spectroscopic/spectrometric/separation analysis problems
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課程要求 |
本課程的考試沒有補考。學生預定之日程表若與本課程之上課或考試時程有任何衝突,切勿修習本課程。學生若因身體不適而錯過考試,則應提出就診紀錄。
學生出席聽講,且課堂上參與授課內容的討論,是學習的重要過程,對教材深入的了解極有幫助。雖然本課程不會每天監看出席,但是若學生無法規律上課,則請勿選修這門課。講義僅當次課堂分發。隨堂摘寫授課筆記是終身學習中的重要技能;學生應隨堂自行摘寫上課筆記。課堂中不得照相攝影,亦不得使用手機。 |
預期每週課後學習時數 |
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Office Hours |
每週五 12:10~13:10 備註: It starts right after the class and ends whenever the last student
finishing asking his/her questions. |
指定閱讀 |
Essential readings:
Holler, Skoog, and Crouch, Principles of Instrumental Analysis, 6th ed., Thomson Brooks/Cole, ISBN: 0-495-01201-7
Daniel C. Harris, Exploring Chemical Analysis, 5th ed., W.H. Freeman, ISBN: 978-1-4292-7503-3 |
參考書目 |
Skoog, West, Holler, Crouch, Fundamentals of Analytical Chemistry, 8th Ed.,
Thomson, ISBN: 0-534-41797-3
James W. Robinson, Undergraduate Instrumental Analysis, Marcel Dekker
Francis Rouessac, Annick Rouessac, Chemical Analysis-Modern Instrumentation
Methods and Techniques, 2nd Ed., Wiley, ISBN: 978-0-470-85902-5
K. A. Rubinson, J. F. Rubinson, Contemporary Instrumental Analysis, Prentice-
Hall, Inc.
Daniel C. Harris, Quantitative Chemical Analysis, 8th Ed., W.H. Freeman and
Company
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評量方式 (僅供參考) |
No. |
項目 |
百分比 |
說明 |
1. |
Final Exam |
42% |
Make-up exams will not be permitted to those who miss the midterms or the final. The content of the tests includes the materials discussed in your textbook as well as those taught in the class. The final test is a comprehensive exam and will require you to integrate the class material in a meaningful way. Calculators used in the exam cannot contain any dictionary function. Cell phones and any other communication devices should be kept away from the exam. Anyone who only sits in the class is not allowed to take the midterm or the final. |
2. |
Quizzes |
0% |
Make-up exams will not be permitted to those who miss the midterms or the final. 學生在不定期舉行的隨堂考中的表現,將做為微調學生之學期成績的參考依據。學生學期成績在通過邊緣者,將檢視審查其上課筆記,酌予微調其學期成績。 |
3. |
Two Midterm Exams |
58% |
兩次期中考成績較佳者佔學期成績34%,較差者佔24%。Make-up exams will not be permitted to those who miss the midterms or the final. The content of the tests includes the materials discussed in your textbook as well as those taught in the class. Calculators used in the exam cannot contain any dictionary function. Cell phones and any other communication devices should be kept away from the exam. Anyone who only sits in the class is not allowed to take the midterm or the final. |
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