課程資訊
課程名稱
分析化學二
Analytical Chemistry (Ⅱ) 
開課學期
102-2 
授課對象
理學院  化學系  
授課教師
張哲政 
課號
Chem2008 
課程識別碼
203 21220 
班次
 
學分
全/半年
半年 
必/選修
必帶 
上課時間
星期一3,4(10:20~12:10)星期四2(9:10~10:00) 
上課地點
新302新203 
備註
外系生須經授課教師同意.詳化學系選課須知.須先修普化.
限學士班二年級以上 且 限本系所學生(含輔系、雙修生)
總人數上限:80人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1022Chem2008_AC2 
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課程概述

This course extends the knowledge of the analytical chemistry to include the fundamental principles and application of modern instrumental methods of analysis. The topics covered have applications to many subject areas in Natural Sciences, Life Science and Engineering.
This course is presented in a lecture/discussion format. The content of the course includes:
Part I:
I. Brief Review of Electrochemistry
II. Amperometry
A. Analysis
B. Glucose Electrode
C. Glucose Electrode
D. Blood Glucose Monitor
E. Chormatographic Detector
III. Voltammetry
A. Basic Concepts
a) Electrode Reaction
b) Voltammogram
c) Concentration Profile
d) Types
e) Supporting Electrolyte
f) Nernst Diffusion Layer
g) Steady-State Current
h) i - E Relationship
B. Microelectrode
a) Disk and ring-disk electrodes
b) Hg electrodes
c) Chemically modified electrodes
C. Hydrodynamic Voltammetry
a) Sampled Current Voltammetry
b) E½ & n
c) i - E Relationship
d) Anodic and Mixed Anodic/Cathodic Voltammogram
D. Stationary Solution Voltammetry
a) Polarography
b) Cyclic Voltammetry (CV)
i) Excitation Signal
ii) Scan Rate
iii) Cyclic Voltammogram
iv) CV Analysis
c) Pulse Voltammetric Methods
i) Differential Pulse Voltammetry
ii) Square Wave Voltammetry
d) Stripping Methods

Part II:
I. Introduction to Instrumental Analysis
II. Fundamentals of Spectroscopy/Spectrometry
A. Introduction to Spectroscopy
B. Characteristics of Electromagnetic radiation
C. Wave Property of Light and Optical Component
a) Interference
b) Transmission
c) Reflection
d) Refraction
e) Scattering
f) Diffraction
D. Particle Property of Light and Its Use
a) Photoelectric effect
b) Absorption
c) Emission
E. Spectroscopy
a) Type of Spectroscopy
b) Nature of Excitation
III. UV and Visible Absorption Spectrometry
A. Range and Type of UV/Vis Radiation
B. Principle
a) Molar Absorptivity
b) Spectroscopic Process
c) Effect of Multichromophores
d) d, f-electron Transition
C. Measurement
a) Standard Addition Method
b) Solvent Selection
c) Solvent Effect
d) Beer’s Law and Its Limitations
e) Spectrophotometric Uncertainty
D. Instrumentation
a) Single-Beam and Double-Beam Instruments
b) Sample Cell and Construction Materials
c) Source
d) Slit
e) Detector
i) Phototube
ii) Photodiode
iii) Photomultiplier Tube
iv) Photovoltaic Cell
v) Multichannel Detector
E. Spectroscopic Information
a) Imaging
b) Qualitative
c) Quantative
F. Spectral Analysis
a) Woodward-Fieser Rule
IV. Luminescence Spectroscopy
A. Luminescence
B. Principle
a) Spectroscopic Process
b) Quantum Yield
c) Favorable Condition for Luminescence
d) Emission Spectrum
e) 90˚ Detection
f) Luminescence Intensity
g) Type of Luminescence
C. Instrumentation
D. Spectroscopic Information
a) FL System
b) Fluorescent Agent
c) Use of Excited-State Molecules
d) Fluorescence Resonance Energy Transfer
V. Infrared Spectroscopy
A. Range and Type of IR Radiation
B. Principle
a) Molecular Excitation
b) Type of Molecular Vibration
c) Normal Mode of Vibration
d) Spectroscopic Process
e) IR-Active Vibration
C. Instrumentation
a) Nondispersive Instrument
b) Dispersive Instrument
c) Sample Cell and Construction Materials
d) Source
e) Wavelength Selector
f) Detector
D. Spectroscopic Information
a) Functional Group
b) Quantitative
VI. Raman Spectroscopy
A. Principle
a) Polarizability
b) Raman Scattering
c) Energy Shift
d) Raman-Active Vibration
e) Raman Scattering Intensity
f) Advantage Over IR
B. Instrumentation
a) Laser
C. Spectroscopic Information
Raman vs. IR
VII. Nuclear Magnetic Resonance Spectroscopy
A. Principle
a) Range of Radiation
b) Nuclear Spin
B. Instrumentation and Measurement
a) Phase Coherence
b) Magnetic Resonance
c) Secondary Field
VIII. Atomic Absorption Spectroscopy
A. Principle
a) Emission
b) Atomic Structure
B. Measurement
a) Source of Errors - Uncertainty Principle
b) Spectral Line Shape
c) Temperature Effect
d) Interference
C. Instrumentation
a) System
b) Atomizer
IX. Analytical Separation
A. General Description
a) Solvent Extraction
b) Mobile and Stationary Phases
c) Classification
d) Elution
B. Principle
a) Migration Rate
b) Retention Time
c) Flow Rate – Flow Velocity Relationship
d) Migration Rate - Distribution Constant
e) Retention Factor
f) Selectivity Factor
g) Band Broadening
h) Column Efficiency
i) Column Resolution
j) Column Resolution
X. Gas Chromatography
A. Separation Process
B. Injection and Detection
C. Detector
D. Sample Preparation
XI. High-Performance Liquid Chromatography
A. Chromatographic Process
B. Injection and Detection
C. Reversed-Phase Separation
D. Gradient Separation 

課程目標
This course provides students with the background theory and operation principles of chemical analysis methods. The specific objectives of the course are as follows:
◎to become familiar with the various spectroscopy, spectrometry, and separation methods currently available
◎to develop an understanding of the theories upon which the principles of the common spectroscopic, spectrometric, and separation procedures are based
◎to understand the correlations of the spectroscopic/spectrometric/separation analysis concepts and of various controllable and measurable quantities
to learn how the different spectroscopic/spectrometric/separation analysis methods are used to analyze samples
◎to understand the limitations and strengths of particular analysis approaches
◎to gain skill and competence in the use of related analytical standards/references
◎to learn to apply chemical equilibria and stoichiometry to solve spectroscopic/spectrometric/separation analysis problems
 
課程要求
本課程的考試沒有補考。學生預定之日程表若與本課程之上課或考試時程有任何衝突,切勿修習本課程。學生若因身體不適而錯過考試,則應提出就診紀錄。
學生上課出席,且課堂上參與授課內容的討論,對教材深入的了解極有幫助。雖然本課程不會每天監看出席,但是若學生無法規律上課,則請勿選修這門課。講義僅當次課堂分發。學生應隨堂自行摘寫上課筆記。課堂中不得照相攝影。
 
預期每週課後學習時數
 
Office Hours
每週四 10:10~11:10
每週一 12:10~13:10 備註: It starts right after the class and ends whenever the last student finishing asking his/her questions. 
指定閱讀
基礎閱讀: Skoog, West, Holler, Crouch, Fundamentals of Analytical Chemistry, 8th Ed., Thomson, ISBN: 0-534-41797-3
修習必讀: Holler, Skoog, and Crouch, Principles of Instrumental Analysis, 6th Ed., Thomson Brooks/Cole, ISBN: 0-495-01201-7
 
參考書目
James W. Robinson, Undergraduate Instrumental Analysis, Marcel Dekker
Francis Rouessac, Annick Rouessac, Chemical Analysis-Modern Instrumentation
Methods and Techniques, 2nd Ed., Wiley, ISBN: 978-0-470-85902-5
K. A. Rubinson, J. F. Rubinson, Contemporary Instrumental Analysis, Prentice-
Hall, Inc.
Daniel C. Harris, Quantitative Chemical Analysis, 8th Ed., W.H. Freeman and
Company
 
評量方式
(僅供參考)
 
No.
項目
百分比
說明
1. 
Final Exam 
42% 
Make-up exams will not be permitted to those who miss the midterms or the final. Do not take this course if you have a class/activity schedule that is in some way in conflict with our class and exam schedules. Students should present records of clinic visits for missing the exam due to illness. The final test is a comprehensive exam and will require you to integrate the class material in a meaningful way. Calculators used in the exam cannot contain any dictionary function. Cell phones and any other communication devices should be kept away from the exam. Anyone who only sits in the class is not allowed to take the midterm or the final. Additional bonus grades will be given on class notes for students with semester grades on the pass-fail border. 
2. 
Quizzes 
0% 
Bonus grades will be given based on the student’s performance in the pop quiz, which will be held on an irregular basis. Additional bonus grades will be given on class notes for students with semester grades on the pass-fail border. Make-up exams will not be permitted to those who miss the midterms or the final. Do not take this course if you have a class/activity schedule that is in some way in conflict with our class and exam schedules. Students should present records of clinic visits for missing the exam due to illness. 
3. 
Two Midterm Exams 
58% 
The better mid-term performance weights 34% and the other weights 24%. Make-up exams will not be permitted to those who miss the midterms or the final. Do not take this course if you have a class/activity schedule that is in some way in conflict with our class and exam schedules. Students should present records of clinic visits for missing the exam due to illness. Calculators used in the exam cannot contain any dictionary function. Cell phones and any other communication devices should be kept away from the exam. Anyone who only sits in the class is not allowed to take the midterm or the final. Additional bonus grades will be given on class notes for students with semester grades on the pass-fail border. 
 
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