課程資訊
課程名稱
分析化學三
ANALYTICAL CHEMISTRY(Ⅲ) 
開課學期
99-1 
授課對象
理學院  化學系  
授課教師
張哲政 
課號
Chem3003 
課程識別碼
203 21230 
班次
 
學分
全/半年
半年 
必/選修
必帶 
上課時間
星期四2(9:10~10:00)星期五5,6(12:20~14:10) 
上課地點
化210室化210室 
備註
本課程中文授課,使用英文教科書。本課程中文授課,使用英文教科書。五選三必修,不修自行退選.詳選課須知.外系需經教師同意.先修:分析化學一二.
限學士班三年級以上
總人數上限:70人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/991Chem3030 
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課程概述

This course extends the knowledge learned in sophomore analytical chemistry to include the fundamental principles and practical application of instrumental transducers used in modern instrumental methods for quantitative determination of chemical compounds. The topics covered have applications to closely allied fields in Natural Sciences, Life Science and Engineering.
This course is presented in a lecture/discussion format. The content of the course includes:
I. Instrumental Approach to Analytical Problems
a) Instrumental methods – classification
b) Methodology of instrumental analysis
c) Reproducibility and interference
d) Spectroscopy vs. spectrometry
e) Quality control
II. DC Circuit
a) Wheatstone bridge
b) Kirchhoff’s laws
III. AC Circuit
a) Signal comparison
b) Impedance
c) RC circuit
d) Voltage divider
e) High-pass and low-pass filter
f) Charging and discharging
IV. Diodes
a) Energy band
b) P-N junction
V. Transistors
a) Bipolar junction transistor
b) Common emitter transistor
c) Characteristic curve
d) Load line analysis
VI. Operational Amplifier
a) Feedback
b) Signal amplification
c) Instrument control
d) Transfer function
e) Voltage follower circuit
f) Inverting amplifier circuit
g) Non-inverting amplifier circuit
h) Difference amplifier circuit
i) Summing amplifier circuit
j) Voltage average circuit
k) Voltage integrator circuit
l) Voltage differentiator circuit
m) Current–to-voltage amplifier circuit
VII. Fundamentals of Spectroscopy/Spectrometry
a) Electromagnetic radiation
b) Interference
c) Transmission
i) Numerical aperture
d) Reflection
i) Reflectance and transmittance
ii) Low-reflection coating
e) Refraction
i) Snell's law
ii) Dispersion coefficient
iii) Chromatic aberration
f) Scattering
i) Lens & prism
ii) Rayleigh scattering
iii) Mie scattering
g) Diffraction
i) Bragg’s law
ii) Grating & filter
h) Angular dispersion vs. linear dispersion
i) Resolving power
j) Effective bandwidth
k) Photoelectric effect
l) Absorption
i) Franck-Condon principle
m) Emission
i) Kasha's rule
ii) Blackbody radiation
iii) Wien's displacement law
iv) Stefan–Boltzmann law
n) Limitations of Beer’s law
o) Spectrophotometric uncertainty
VIII. Signals and Noise
a) Sources of noise
b) Signal-to-noise enhancement
IX. UV/Vis Spectrometry
a) Spectroscopic process
b) Molar absorptivity
c) Chromophore type
d) Multichromophore effect
e) Solvent effect
f) Auxochromic effect
g) Woodward-Fieser rule
X. Luminescence Spectroscopy
a) Spectroscopic process
b) Types of luminescence
c) Photoluminescence
d) Synchronous spectra
e) Chemiluminescence
f) Bioluminescence
g) Electroluminescence
h) Laser
i) Fluorescent agent
j) Luminescence quenching
k) Fluorescence resonance energy transfer
XI. Nuclear Magnetic Resonance Spectroscopy
a) Nuclear spin
b) Spin angular momentum
c) Spin quantum number
d) Spin magnetic moment
e) Screening constant
f) Excitation
g) Chemical shift
h) Spin-spin splitting
i) Coupling constant
j) Hydrogen exchange effect
k) Relaxation mode
l) Instrumentation
m) Phase coherence
n) Spectroscopic information
XII. Electron Spectroscopy
a) X-ray Photoelectron Spectroscopy
i) Principle
ii) Instrumentation
iii) Mean free path
iv) Angular dependence
v) Sampling depth
vi) Overlayer
vii) Spin-orbit coupling
viii) Chemical information
b) Auger Electron Spectroscopy
i) Principle
ii) Instrumentation
iii) Analytical volume
iv) Attenuation length
v) Chemical information
XIII. Mass Spectrometry
a) Ion source
b) Mass analyzer
c) Mass spectrum
d) Inductively coupled plasma-mass spectroscopy
e) Secondary ion mass spectroscopy
XIV. Bioanalysis
a) Fluoroimmunoassay
b) ELISA methodology
c) Biochip
d) Western blotting
e) Electrophoresis (PAGE)
f) Selective molecular assembly patterning
g) Surface plasmon resonance
XV. Thermal Analysis
a) Thermogravimetric analysis
b) Differential thermal analysis
c) Differential scanning calorimetry
 

課程目標
This course is an advanced-level undergraduate course that covers the fundamental concepts and background principles about chemical instrumentation of analytical methods. Instrumental theories and concepts are introduced to provide a framework for discussions of the advantages and disadvantages offered by various transducer systems used for chemical analysis. The specific objectives of the course are that the student be able to:
- gain working knowledge of analytical instrumentation typically employed in chemical laboratories;
- comprehend the basic scientific concepts and operational principles involved in analytical instrumentation;
- describe the layout and components of prototypical instruments;
- understand instrument design and construction;
- integrate a fundamental understanding of the underlying theories upon which the principles of optical and particle spectroscopic and spectrometric procedures are based;
- relate optical and electronic properties to quantitation of analytes in different sample matrices;
- understand the limitations and strengths of different instrument components and methods;
- apply and assess concepts of availability and evaluation of analytical standards/references;
- recognize interferences and assess sources of error in instrumental analysis;
- select appropriate instrumental methods for addressing analytical problems.
 
課程要求
a) This course presumes some prior exposure to classical chemical analyses and general instrumental analyses, as well as knowledge of basic physics and electronics.
b) Make-up exams will not be permitted to those who miss the midterms or the final. Students should present records of clinic visits for missing the exam due to illness.
c) Good, participatory attendance is essential to successful mastery of course material. Although the attendance will not be monitored daily, it is suggested that you do not take this course if you cannot come to the class.
d) Photography and videotaping of any form are not allowed in the classroom.
e) Handouts are given only in the current class session.
 
預期每週課後學習時數
 
Office Hours
每週四 10:00~11:00
每週五 15:10~16:10 備註: The session starts right after the lecture and ends when the last student leaves. 
參考書目
James W. Robinson, Undergraduate Instrumental Analysis, Marcel Dekker
Francis Rouessac, Annick Rouessac, Chemical Analysis-Modern Instrumentation Methods and Techniques, 2nd Ed., Wiley, ISBN: 978-0-470-85902-5
K. A. Rubinson, J. F. Rubinson, Contemporary Instrumental Analysis, Prentice-Hall, Inc.
Daniel C. Harris, Quantitative Chemical Analysis, 7th Ed., W.H. Freeman and Company
Skoog, West, Holler, Crouch, Fundamentals of Analytical Chemistry, 8th Ed., Thomson, ISBN: 0-03-035523-0
 
指定閱讀
Optional Text : Holler, Skoog, and Crouch, Principles of Instrumental Analysis, 6th Ed., Thomson Brooks/Cole, ISBN: 0-495-01201-7 
評量方式
(僅供參考)
 
No.
項目
百分比
說明
1. 
Project sets 
20% 
Due on the deadline before the class starts; 20% deduction for late turn-ins thereafter; 30% deduction for turning in after 7 days; 40% deduction for turning in after 14 days; and 60% deduction for turning in after 21 days; and 100% deduction for turning in after Jan 10. 
2. 
First Midterm 
25% 
The exams are primarily problems and short essays, not multiple choice. They will emphasize the course objectives of understanding the background theory, operation principles, design, applications, strengths, and limitations of chemical instrumentation and analysis methods. The final test is a comprehensive exam and will require you to integrate the class material in a meaningful way. Calculators used in the exam cannot contain any dictionary function. Anyone who only sits in the class is not allowed to take the midterm or the final.  
3. 
Second Midterm 
25% 
The exams are primarily problems and short essays, not multiple choice. They will emphasize the course objectives of understanding the background theory, operation principles, design, applications, strengths, and limitations of chemical instrumentation and analysis methods. The final test is a comprehensive exam and will require you to integrate the class material in a meaningful way. Calculators used in the exam cannot contain any dictionary function. Anyone who only sits in the class is not allowed to take the midterm or the final.  
4. 
Final Exam 
30% 
The exams are primarily problems and short essays, not multiple choice. They will emphasize the course objectives of understanding the background theory, operation principles, design, applications, strengths, and limitations of chemical instrumentation and analysis methods. The final test is a comprehensive exam and will require you to integrate the class material in a meaningful way. Calculators used in the exam cannot contain any dictionary function. Anyone who only sits in the class is not allowed to take the midterm or the final.  
5. 
Quizzes 
0% 
Bonus grades will be given based on the performance in the pop quiz, which will be held on an irregular basis, for students with semester grades on the borderline. 
 
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