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課程名稱 |
單分子化學生物學方法
METHODS IN SINGLE MOLECULE CHEMICAL BIOLOGY |
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開課學期 |
97-1 |
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授課對象 |
理學院 化學所化學生物學組 |
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授課教師 |
李弘文 |
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課號 |
Chem5059 |
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課程識別碼 |
223 U2660 |
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班次 |
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學分 |
2 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期四3,4(10:20~12:10) |
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上課地點 |
化121 |
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備註 |
初選不開放。本課程中文授課,使用英文教科書。詳選課須知。外系所學?
限學士班三年級以上 且 限本系所學生(含輔系、雙修生)
總人數上限：20人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/971_sm |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
Section 1: Principles of single-molecule techniques and analysis
- Optical microscopy
- Single molecule fluorescence and single pair FRET
- Optical tweezers, magnetic tweezers methods
- Other novel method development
- Single-molecule data analysis
- Laboratory tour of single-molecule lab
Section 2: Recent significant publications to illustrate how use single molecule methods are used to investigate biochemical mechanisms.
At the end of the second section, students are required to conduct a research project using single molecule methods to address biological mechanisms.
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課程目標 |
This course will present an overview of current experimental state-of-art single molecule techniques used in chemical biology and biophysics. Emphasis will be on two areas: (1) novel single molecule instrumentation and measurement methods, and (2) application of single molecule methods to study the mechanisms of chemical biology processes. |
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課程要求 |
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預期每週課前或/與課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
mid-term |
30% |
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2. |
class participation |
25% |
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3. |
research project |
45% |
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- 本校建議 A+ 比例上限為 20% ，非強制規定，授課教師可依課程要求調整，建議必修課程參考。
- 本校採用等第制評定成績，學生成績評量辦法中的百分制分數區間與單科成績對照表僅供參考，授課教師可依等第定義調整分數區間。詳見學習評量專區 (連結)。
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週次 |
日期 |
單元主題 |
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第01週 |
09/18 |
Single molecule introduction |
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第02週 |
09/25 |
DNAzymes (1)
--Ha, T. et al., “Initiation and Re-initiation of DNA Unwinding by the Echerichia Coli Rep Helicase”, Nature 419, 638-641 (2002).
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第03週 |
10/02 |
DNAzymes (2)
--Dohoney KM, Gelles J.,”Chi-sequence recognition and DNA translocation by single RecBCD helicase/nuclease molecules.” Nature, 409, 370-4 (2001).
--Spies M et al., “RecBCD enzyme switches lead motor subunits in response to chi recognition.” Cell, 131, 694-705 (2007). |
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第04週 |
10/09 |
DNAzymes (3)
--Lee JB et al., “DNA primase acts as a molecular brake in DNA replication.” Nature, 439, 621-4 (2006).
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第05週 |
10/16 |
DNAzymes (4)
--Strick TR et al., “Single-molecule analysis of DNA uncoiling by a type II topoisomerase” Nature, 404, 901-4 (2000).
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第06週 |
10/23 |
Kinesin (1)
--Hua W et al., “Distinguishing inchworm and hand-over-hand processive kinesin movement by neck rotation measurements.” Science, 295, 844-8 (2002).
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第07週 |
10/30 |
Kinesin (2)
--Yildiz A et al., “Kinesin walks hand-over-hand.” Science, 303, 676-8 (2004).
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第08週 |
11/06 |
Myosin (1)
--Forkey JN et al., “Three-dimensional structural dynamics of myosin V by single-molecule fluorescence polarization.” Nature, 422, 399-404 (2003).
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第09週 |
11/13 |
~Mid-term Week (research project, no class)~ |
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第10週 |
11/20 |
Myosin (2)
--Shiroguchi K, Kinosita K Jr., ”Myosin V walks by lever action and Brownian motion.” Science, 316, 1208-12 (2007).
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第11週 |
11/27 |
RNA Polymerase
--Abbondanzieri EA et al., “Direct observation of base-pair stepping by RNA polymerase.” Nature, 438, 460-5 (2005).
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第12週 |
12/04 |
Protein Folding
--Fernandez JM, Li H., “Force-clamp spectroscopy monitors the folding trajectory of a single protein” Science, 303, 1674-8 (2004).
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第13週 |
12/11 |
Take-home Mid-term |
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第14週 |
12/18 |
(research project, no class) |
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第15週 |
12/25 |
(research project, no class) |
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第16週 |
2009/01/01 |
Holiday |
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第17週 |
2009/01/08 |
Research Project Presentation (20 min per group) |
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第18週 |
2009/01/25 |
(research project wrap-up, no class) |
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