課程名稱 |
高等化學生物學一 Advanced Chemical Biology (Ⅰ) |
開課學期 |
101-1 |
授課對象 |
理學院 化學所化學生物學組 |
授課教師 |
戴桓青 |
課號 |
Chem7037 |
課程識別碼 |
223EM1660 |
班次 |
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學分 |
4 |
全/半年 |
半年 |
必/選修 |
必修 |
上課時間 |
星期一7,8(14:20~16:20)星期四5,6(12:20~14:10) |
上課地點 |
化217化217 |
備註 |
本課程以英語授課。限碩士生修習。 總人數上限:50人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1011adv_chem_bio_1 |
課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
課程大綱
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課程概述 |
1. Proteins
2. Nucleic Acids
3. Enzyme Catalysis
4. Carbohydrates
5. Lipids and Membranes
6. Characterization of biomolecules
7. Separation and identification methods, including chromatography and electrophoresis
8. Hydrodynamic methods, including density and molecular volume, ultracentrifugation, sedimentation, diffusion and Brownian motion, and dynamic light scattering
9. Thermodynamics and kinetics methods, including calorimetry, dialysis, protein solubility and crystallization, relaxation, enzyme kinetics, and surface plasma resonance
10. Spectroscopic methods, including UV-Vis spectroscopy, circular dichroism, fluorescence, vibrational spectroscopy (IR and Raman), and NMR spectroscopy
11. Mass spectrometric methods, including ion sources, ionization, mass analyzers, detection, and application
12. Single molecule methods
p.s. The students in this course will be grouped into several teams. In principle, a team will consist of 3–6 students from different disciplines to work together. Each team will be assigned a small project to finish before oral presentation; that is, each team must submit a written report (3 hard copies) and a powerpoint electronic file for oral presentation to Professor Fang by December X. The written report must include description of each slide presented in the powerpoint file. All these materials will be posted on the course website. Only one student in each team will be drawn on site as the team representative to give oral presentation
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課程目標 |
1. Proteins
2. Nucleic Acids
3. Enzyme Catalysis
4. Carbohydrates
5. Lipids and Membranes
6. Characterization of biomolecules
7. Separation and identification methods, including chromatography and electrophoresis
8. Hydrodynamic methods, including density and molecular volume, ultracentrifugation, sedimentation, diffusion and Brownian motion, and dynamic light scattering
9. Thermodynamics and kinetics methods, including calorimetry, dialysis, protein solubility and crystallization, relaxation, enzyme kinetics, and surface plasma resonance
10. Spectroscopic methods, including UV-Vis spectroscopy, circular dichroism, fluorescence, vibrational spectroscopy (IR and Raman), and NMR spectroscopy
11. Mass spectrometric methods, including ion sources, ionization, mass analyzers, detection, and application
12. Single molecule methods
p.s. The students in this course will be grouped into several teams. In principle, a team will consist of 3–6 students from different disciplines to work together. Each team will be assigned a small project to finish before oral presentation; that is, each team must submit a written report (3 hard copies) and a powerpoint electronic file for oral presentation to Professor Fang by December X. The written report must include description of each slide presented in the powerpoint file. All these materials will be posted on the course website. Only one student in each team will be drawn on site as the team representative to give oral presentation
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課程要求 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
“Biochemistry” Sixth Edition, by Jeremy M. Berg, John L. Tymoczko, and Lubert
Stryer, W. H. Freeman and Company, NewYork, New York, 2006.
“Principles of Physical Biochemistry” Second Edition, by Kensal E. van Holde,
Curtis Johnson, and Pui Shing Ho, Pearson Education International, 2006. |
評量方式 (僅供參考) |
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週次 |
日期 |
單元主題 |
Week 1 |
9/10,9/13 |
1. Syllabus
2. Comments on the life sicence job market |
Week 2 |
9/17,9/20 |
1. Introduction to chemical biology
2. Nucleic acid sturcture and synthesis |
Week 3 |
9/24,9/27 |
1. DNA sequencing and DNA-related chemistry
2. RNA interference |
Week 4 |
10/01,10/04 |
1. Ribozymes and aptamers
2. Carbohydrate structure |
Week 5 |
10/08,10/11 |
Glycan function |
Week 6 |
10/15,10/18 |
Lipids and Membranes |
Week 7 |
10/22,10/25 |
Midterm exam |
Week 8 |
10/29,11/01 |
Protein synthesis and folding |
Week 9 |
11/05,11/08 |
1. Protein unfolding and misfolding
2. Protein oxidation |
Week 10 |
11/12,11/15 |
Midterm discussion |
Week 11 |
11/19,11/22 |
1. Protein degradation and quality control
2. Protein production and separation |
Week 12 |
11/26,11/29 |
1. Crystallography, NMR, cryo-EM
2. Cellular protein extraction |
Week 13 |
12/03,12/06 |
1. antibody technology, humanized antibody
2. Labeling with biotin and flurophores |
Week 14 |
12/10,12/13 |
1. Guest lecture: Single Molecule Fluorescence 2. Bioorthogonal chemistry |
Week 15 |
12/17,12/20 |
Protein mass spectrometry: instrumentation and sample preparation |
Week 16 |
12/24,12/27 |
Mass spec applications: quantitation and PTM |
Week 17 |
12/31,1/03 |
1. Term paper due
2. Final review |
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