週次 |
日期 |
單元主題 |
第1週 |
2/22 |
Molecular identification (I):
DNA extraction of gram-positive bacteria |
第2週 |
3/01 |
Molecular identification (II):
PCR of groESL gene |
第3週 |
3/08 |
Genotyping of MRSA |
第4週 |
3/15 |
Molecular identification (III):
RFLP of groESL amplicon |
第5週 |
3/22 |
Exam I |
第6週 |
3/29 |
Reporter assay(I): reporter plasmid construction- preparation of plasmids |
第8週 |
4/12 |
Report assay (II) : reporter plasmid construction- preparation of insert and vector and ligation |
第9週 |
4/19 |
Report assay (III)- preparation of competent cells and transformation |
第10週 |
4/26 |
Report assay (IV)-identification of transformants and determination of reporter activity |
第11週 |
5/03 |
Exam II |
第12週 |
5/10 |
Bioinformatics in bacterial genome analysis (I) |
第13週 |
5/17 |
Bioinformatics in bacterial genome analysis (II) |
第14週 |
5/24 |
Next-generation sequencing in bacteria |
第15週 |
5/31 |
Antibiotic Synergy Test: Microdilution Checkboard Assay (I) |
第16週 |
6/07 |
Antibiotic Synergy Test: Microdilution Checkboard Assay (II) |
第17週 |
6/14 |
Pulsed-field gel electrophoresis (Demo) |