課程資訊
課程名稱
分子生物學
Molecular Biology 
開課學期
101-1 
授課對象
醫學院  免疫學研究所  
授課教師
林敬哲 
課號
md&ph5001 
課程識別碼
420 U0100 
班次
 
學分
全/半年
半年 
必/選修
選修 
上課時間
星期二8,9星期四8,9 
上課地點
基醫301基醫301 
備註
本課程中文授課,使用英文教科書。與胡務亮、方偉宏、俞松良、陳培哲、楊偉勛、周綠蘋、林榮耀、張明富、張富雄、呂紹俊、游偉絢、李明學、余明俊、張 鑫、董馨蓮、陳美如、鄧述諄、李財坤、葉秀慧、詹世鵬、楊宏志、錢宗良、李芳仁、阮麗蓉、吳君泰、許秉寧、賈景山、張百
總人數上限:200人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1011molbio 
核心能力關聯
本課程尚未建立核心能力關連
課程大綱
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課程概述

見醫衛共同課程時間表 

課程目標
讓研究基礎醫學的研究生,瞭解原核生物與真核細胞內,核酸與蛋白質
等巨分子的結構與功能,以奠定其醫學研究的基礎。
 
課程要求
 
Office Hours
 
參考書目
 
指定閱讀
 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
9/11,9/13  (I)Introduction (II)DNA structure and function (1)DNA as store of information (A) Outline of major experiments that led to the acceptance of DNA as the genetic material (B) Structure of nucleic acid, base pairing, tautomeric shift, DNA-A,-B,-Z forms (C) Denaturing and renaturing of DNA (D) Supercoiled DNA and topoisomerase 
第2週
9/18,9/20  (2) DNA technology, genomic size and genetic content (3) Genome: organization and evolution 
第3週
9/25,9/27  (4) Extrachromosome DNA, nucleosome, chromosome and chromatid (5) DNA replication (A) Prokaryotic system 
第4週
10/02,10/04  (B) Eukaryotic system (C) Telomere (6) The dynamic genome (A) Recombination and transposition of DNA in the prokaryotic genome (B) Rearrangement and amplification in the eukaryotic genome 
第5週
10/09,10/11  (7)DNA damage and mutation (8)DNA repair and modification 
第6週
10/16,10/18  第一次期中考 (考試範圍 9/11~10/11) (III)Transcription, regulation and processing (1) Prokaryotic Transcription and Eukaryotic Pol II Transcription 
第7週
10/23,10/25  (2) Transcription and Epigenetic Regulation (3) Eukaryotic transcription and regulation (eukaryotic RNA pol. I and III) 
第8週
10/30,11/01  (4) Post-transcriptional processing, ribozyme and regulation (IV)Translation (1) Assembly line for protein synthesis (2) Transfer RNA is the translated adaptor 
第9週
11/06,11/08  (3) Ribosomes provide a translation factory (4) Codons and their significance (5) Translation in cell free system (6)未確定 
第10週
11/13,11/15  (7) Post-translational modification I. Structure II.Protein ubiquitination (V)Recombinant DNA technology(I)  
第11週
11/20,11/22  (1) Cloning of DNA:transformation and screening of transformants (2) Construction of cDNA and genomic libraries (3) Colony and plaque hybridization, Southern blot, Northern blot, Western blot analysis (4) Primer extension, S1 nuclease mapping and RNase protection assay (5) Differential display (6) Site-directed mutagenesis and polymerase chain reaction (7) Application of restriction fragment length polymorphism and variable number tandem repeat for genetic disease analysis (8) Genome-wide sequencing strategies 
第12週
11/27,11/29  (9) Phage strategies : control of lytic and lysogenic pathway (10) Phage display library (11) A brief introduction to animal viruses 第二次期中考(考試範圍 10/18~11/22) (VI)Construction of the cells 
第13週
12/04,12/06  (1) Signal transduction in prokaryotes (2) Signal transduction in eukaryotes 張富雄 (VII)Genes in the development (1) Immune diversity and immunotherapy 
第14週
12/11,12/13  (2) Gene regulation and development (3) Oncogenes (4) Tumor suppressor genes 
第15週
12/18,12/20  (VIII)Recombinant DNA technology(II) (1) Expression of cloned gene in prokaryotic and eukaryotic cells (2) Yeast genetics and its applications (3) Applications of bioinformatics and proteomics in biomedical research 
第16週
12/25,12/27  (4) Current development of genome projects and their impacts to human diseases (5) Transgenic techniques 
第17週
1/01,1/03  (6) Gene therapy (A) Viral vectors (B) Clinical Application 期末考 (考試範圍 12/04~01/03)