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Course title |
Molecular Biotechnology |
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Semester |
106-1 |
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Designated for |
COLLEGE OF MEDICINE GRADUATE INSTITUTE OF ORAL BIOLOGY |
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Instructor |
BEI-EN CHANG |
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Curriculum Number |
OraBio7263 |
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Curriculum Identity Number |
450 M2630 |
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Class |
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Credits |
2.0 |
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Full/Half
Yr. |
Half |
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Required/
Elective |
Elective |
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Time |
Friday 4,5(11:20~13:10) |
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Remarks |
The upper limit of the number of students: 20.
The upper limit of the number of non-majors: 5. |
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Ceiba Web Server |
http://ceiba.ntu.edu.tw/1061OraBio7263_ |
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Course introduction video |
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Table of Core Capabilities and Curriculum Planning |
Table of Core Capabilities and Curriculum Planning |
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Course Syllabus
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Please respect the intellectual property rights of others and do not copy any of the course information without permission
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Course Description |
一、課程簡介:
1. Web
2. I.Enzymology of recombinant DNA-Restriction enzymes
3. II.Enzymology of recombinant DNA-Enzymes in DNA engineering
4. Isolation of nucleic acids, blotting and DNA sequencing
5. Plasmids and cloning strategies
6. Bacteriophage, cosmid, YAC
7. (Mid-term examination)
8. Primer design, PCR strategies, RACE
9. Hybridization probes and screening
10. Analyses of gene regulation
11. Construction of cDNA and genomic DNA libraries
12. Expression systems and purification
13. Transgenic animal and gene targeting
14. whole-mount in situ hybridization and other techniques
二、先修課程:
三、參考書目:1.Greene, J. J. and Rao, V. B. 1998 Recombinant DNA Principles and Methodologies.
2.Old, R. W. and Primrose, S. B. 2002 Principles of Gene Manipulation.
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Course Objective |
課程定位: 分子生物學技術, 為實驗室內可運用之基礎課程。主要課程內容是介紹近代分子生物學技術層面之發展，以及在實驗室常用的分子生物學技術之基本原理、方法。因此，本課程著重在實際一般實驗操作過程中可能會應用到的技術，操作過程可能會遇到的問題。
教學目標: 本課程目的在使學生了解分子生物學之基本操作方法及原理，例如基因之選殖、表現、載體之製備、基因轉殖動物之建立等。
核心能力: 學生修習本課程後，對分子生物學之基本操作方法及原理應有基本之了解，並在實驗室實際操作過程能加以運用，避免錯誤，增加實驗成功之效率。
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Course Requirement |
待補 |
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Student Workload (expected study time outside of class per week) |
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Office Hours |
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Designated reading |
待補 |
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References |
待補 |
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Grading |
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Week |
Date |
Topic |
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第1週 |
9/15 |
Web - Database |
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第2週 |
9/22 |
Endnote and bioinformatics |
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第3週 |
9/29 |
I. Enzymology of recombinant DNA-Restriction enzymes |
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第4週 |
10/06 |
II. Enzymology of recombinant DNA-Enzymes in DNA engineering |
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第5週 |
10/13 |
Isolation of nucleic acids, blotting and DNA sequencing |
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第6週 |
10/20 |
Plasmids and cloning strategies |
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第7週 |
10/27 |
Bacteriophage, cosmid, YAC, BAC |
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第8週 |
11/03 |
期中 |
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第9週 |
11/10 |
PCR strategies, RACE, and primer design |
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第10週 |
11/17 |
Hybridization probes and screening |
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第11週 |
11/24 |
Analyses of gene regulation |
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第12週 |
12/01 |
Construction of cDNA and genomic DNA libraries |
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第13週 |
12/08 |
Protein expression systems and purification |
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第14週 |
12/15 |
Zebrafish genetics and application |
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第15週 |
12/22 |
in situ hybridization |
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