課程資訊
課程名稱
林木組織培養
Tissue Culture of Woody Plants 
開課學期
99-2 
授課對象
生物資源暨農學院  森林環境暨資源學系  
授課教師
王亞男 
課號
Forest3044 
課程識別碼
605 44000 
班次
 
學分
全/半年
半年 
必/選修
必修 
上課時間
星期一3,4,@(10:20~) 
上課地點
林二 
備註
森林生物學群選擇必修
總人數上限:30人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/992tissue_culture 
課程簡介影片
 
核心能力關聯
核心能力與課程規劃關聯圖
課程大綱
為確保您我的權利,請尊重智慧財產權及不得非法影印
課程概述

主題:
1.組織培養技術介紹
2.生長調節物質的作用
3.微體繁殖
4.癒合組織之誘導
5.原生質體培養、莖頂培養
6.林木原生質體之分離、收集及純化、林木原生質體培養
7.林木氮代謝和營養繁殖、營養繁殖與年輕態、成熟態和復壯的相關
8.林木組織培養與林木育種的結合、林木組織培養的問題與展望
9.組織培養苗的馴化與健化



 

課程目標
認識林木組織培養  
課程要求
1.期中期末考
2.平時測驗
3.實驗室實作及報告 
預期每週課後學習時數
 
Office Hours
 
參考書目
1.上野洋一郎、郭榮烈、陳泓志、胡威文譯。2004。圖解植物組織培養入門。藝軒出版
社。(原著:清水碩、□原坦、作田正明)
2.上野洋一郎、溫秋明、科俊良、陳怡菁、郭順宇、郭榮烈、陳滑志、胡威文、張傳偉。
2000。組織培養的技術。藝軒出版社。(原著:黑田行昭)
3.上野洋一郎等/編譯。2000。植物組織培養與技術。藝軒出版社。
4.上野洋一郎等/編譯。植物組織培養技術。藝軒出版社。
5.王國平、洪霓主編。2005。農業生物技術系列。化學工業出版社。(原著:M.K.拉茲丹)
6.奚元齡、顏昌敬編譯。1992。植物細胞培養手冊。農業出版社。
7.郭仲琛、桂耀林主編。1990。植物體細胞胚胎發生和人工種子。科學出版社。
8.劉進平,莫饒著。2006。熱帶植物組織培養。科學出版社。
9.淑馨編輯部/著。1986。圖解組織培養入門。淑馨出版社。
10.淑馨出版社。1986(初)、1991(再)。圖解組織培養入門。(原著:古川仁朗)
11.闕國寧、郭達初、李金田譯。1988。樹木組織培養。中國林業出版社。
12.バイオテクノロツー全書編輯委員會。1989。木本植物的增殖和育種。農學圖書株式會
社。
13. 劉麗飛主編。2003。植物組織培養與基因轉殖。國立台灣大學生物技術研究中心。
14. Acram Taji, Prakash P. Kumar, Prakash Lakshmanan – 2002. In Vitro Plant
Breeding.
15.Ahuja, M.R. 1993. Micropropagation of Woody Plants. Kluwer Academic
Publishero.Dordrecht/Boston/loiidon. A.J. (Antonio J.) Marquez, J. (Jens)
Stougaard – 2005. Lotus Japonicus Handbook.
16.Alka. Narula, P. S. (Prem S.) Srivastava, S. S. (Sant Saran) Bhojwani -
Science – 2004. Plant Biotechnology and Molecular Markers.
17.Bruce Macdonald – 1987. Practical Woody Plant Propagation for Nursery
Growers.
18.B. M. Reed – 2004. Technical Guidelines for the Management of Field and in
Vitro Germplasm Collections. A simplified method for the control of bacterial
contamination in woody plant tissue culture. In vitro Cell. Dev. Biol. 27P:42.
Viterbo, A., HD Rabinowitch.
19.Bajaj, Y.P.S. 1986. Biotechnology in Agriculture and Forestry I .-Trees 1.
Spriiiger-Verlag, Berlin, Heidelberg, New York, Tokyo.
20.Bajaja, Y.P.S. 1991 Biotechnology in Agriculture and Forestry 16.- Trees
III. Springer-Vei-lag, Berlin, Heidelberg, New York. Lond.on, Paris, Tokyo,
Hong Kong, Barcelona, Budapest.
21.Bajaj, Y.P.S. 1991 Biotechnology in Agriculture and Forestry 17.- High-Tech
and Micropropagation 1. Springer-Verlag, Berlin. Heidelberg, New York, London,
Paris, Tokyo,Hong Kong, Barcelona, Budapest.
22.Bonga J.M. and. D.J. Durzan. 1982. Tissue Culture in Forestry. Martinus
Nijhoff/Dr. W.Junk Publishers. Hague/Boston/London.
23.Bonga J. M. and D. J. Durzan 1987. Cell and Tissue Culture in Forestry I II
HI. Martinus Nijhoff. Publishers, Dordrecht.
24.Chongbiao You, Zhangliang Chen, Yong Ding - Technology – 1993.
Biotechnology in Agriculture: Proceedings of the First Asia-Pacific Conference
on Agriculture.
25.Daizy Batish, R. K. Kohli, Harminder Pal Singh – 2006. Handbook Of
Sustainable Weed Management. Woody plants and woody plant management: Ecology,
safety, Plant Cell, Tissue and Organ Culture 66: 97-105. Decks, SJ, SF
Shamoun, and ZK Punja (2002).
26.Daniel Mohan Bienstock, S. Mohan Jain, Katsuaki Ishii – 2003.
Micropropagation of Woody Trees and Fruits.
27.Delbergh, P.C. and R.H. Zimmermar 1991 Micropropagation-Technology and
Application. Kluwer Academic Publishers.
28.Don J. Durzan, J. M. Bonga – 1982. Tissue Culture in Forestry.
29.Don J. Durzan, J. M. Bonga – 1987. Cell and Tissue Culture in Forestry.
30.Dorothy Johnson Callaway – 1994. The world of magnolias.
31.Edited by K Lindsey – Science.1985. Plant Tissue Culture Manual.
32.Evans D.A. W. R. Sharp, P. V. Ammirato and Y. Yamada 1983 Hand book of
Plant Cell. Culture. Vol. 1. Technignes for propagation and Breeding.
Maemillan Publishing Co., New York.
33.Fowke, L.C., and F. Constabel. 1985 Plant Protoplasts. CRC Press, Roca
Ratom.
34.George G. Khachatourians, Alan McHughen – 2002. Transgenic Plants and
Crops. In Vitro Cell Dcv Biol Plant 34:267—275, 1998. 47. CA Huetteman, JE
Preece.
35.Gregory N. Brown, Mary L. Duryea – 1984. Seedling Physiology and
Reforestation Success.
36.Henke R. R. ; K.W. Hughes; M. J. Constantin ; A. Hollaender and. C.M.
Wilson 1985.Tissue Culture in Forestry and Agri.culti.ii-e. Plenum press. New
York; London.
37.Ian S. (Ian Scott) Curtis - Technology – 2004. Transgenic Crops of the
World: Essential Protoools.
38.Indra K. Vasil, Trevor A. Thorpe - Technology . 1994. Plant Cell and Tissue
Culture. 5th International Congress of Plant Tissue and Cell Culture, Tokyo,
Japan.
39.Ioannes Tsekos, Michael Moustakas - Science – 1998. Progress in Botanical
Research.
40.J. M. Bonga, Don J. Durzan. 1987.Cell and Tissue Culture in Forestry.
41.J. M. Bonga, Don J. Durzan – 1987. Cell and Tissue Culture in Forestry,
Volume 3: growth and developments.
42.John. B. M. 1982 Experimental Embryology of Vascular Plants. Springer-
Verlag. Berlin, Heidelberg, New York.
43.John H. Dodds - Nature – 1990. In Vitro Methods for Conservation of Plant
Genetic Resources.
44.Louise E. Buck, James P. Lassoie, Erick C. M. Fernandes – 1999.
Agroforestry in Sustainable Agricultural Systems.
45.Margit Laimer, Waltraud Rucker – 2004.Plant Tissue Culture: 100 Years
Since Gottlieb Haberlandt.
46.M. K. Razdan - Science – 2003. Introduction to Plant Tissue Culture.
47.Mohan Jain, Subhash C. Minocha – 2000. Molecular Biology of Woody Plants
v. 2.
48.Nolan Omri Rawlins, Cliff Ricketts – 1999. Introduction to Agribusiness.
49.Octavio Paredes-López – 1999. Molecular Biotechnology for Plant Food
Production. Plant Cell Rep. 14:753—757. 11 Huetteman, CA and JE Preece. 1993.
50..Pierik R. L. M. 1987 Invitro Culture of Higher Plants Martuius Nijhoff
Publishers.
51.P. “von” Aderkas, J. M. Bonga – 1992. In Vitro Culture of Trees.
52.Robert Nicholas Trigiano, Dennis John Gray. 2000. Plant Tissue Culture
Concepts and Laboratory Exercises.
53.Robert Warmbrodt – 1991. Biotechnology: Genetic Engineering for Crop Plant
Improvement, Bibliography January 89-March 91. The original seed-derived
plants, which had served as donors for tissue culture and were capable of
Breeding systems of woody plant species in Utah.
54.Rodney W. Bovey – 2001. Woody Plants and Woody Plant Management: Ecology,
Safety and Environmental Impact.
55.Sheng-chung Huang – 1992. The Impact of Biological Research on
Agricultural Productivity: Proceedings of the SABRAOPlant Cell Tissue Organ
Culture 1: 275-281. Fujioka, N., K. Nakao.S. Mohan Jain, B. S. Ahloowalia, D.
S. Brar - Technology – 1998. Somaclonal Variation and Induced Mutations in
Crop Improvement.
56.S. Mohan Jain - Gardening – 1995. Somatic Embryogenesis in Woody Plants.
57.S. Mohan Jain, Subhash C. Minocha - Science - 1999 .Molecular Biology of
Woody Plants v. 1. Somatic Embryogenesis in Woody Plants, Vol..
58.S. Mohan Jain, Pramod K. Gupta, Ronald J. Newton – 1999. Somatic
Embryogenesis in Woody. Dordrecht: Kluwer Academic Publishers.
59.S. Mohan Jain, Pramod K. Gupta, Ronald J. Newton – 1999.Somatic
Embryogenesis in Woody Plant tissue culture media. Kluwer Academic Publishers.
60.S. Mohan. Jain, Pramod K. Gupta - Science – 2005. Protocol for Somatic
Embryogenesis in Woody Plants . George EF (1993) Plant Propagation By Tissue
Culture I — The Technology. Abstr. XIII Plant Tissue Culture Conference,
October 18-20, Shillong.
61.Tong-Jen. Fu, Gurmeet. Singh, Wayne R. Curtis – 1999. Plant Cell and
Tissue Culture for the Production of Food Ingredients.
62.T. (Toyoki) Kozai, Jenny Aitken-Christie, Mary Ann Lila Smith. 1995.
Automation and Environmental Control in Plant Tissue Culture.
63.USDA Forest Service, North Central Forest Experiment Station 1994 Second
International Symposium on the Application of Biotechnology to Tree Culture,
Protection and Utilization.
64.Wilson, Keith Wilson – 2005. Principles And Techniques Of Biochemistry And
Molecular Biology.
65.Woong-Young. Soh, S. S. (Sant Saran) Bhojwani - Science – 1999.
Morphogenesis in Plant Tissue Cultures.
66.Y. P. S., Bajaj – Science. 1992. High-Tech and Micropropagation v. 3.In:
Bonga JM, Durzan Di (eds) Cell and tissue culture in forestry, vol 3. Martinus
Nijhoff, Dordrecht, pp 300—315 mccown BH, Lloyd G (1981) Woody plant .
67.Yashpal Singh Bajaj – 1989. Trees II.
68.Yashpal Singh Bajaj - Science – 1989. Plant Protoplasts and Genetic
Engineering I.
69.Y. P. S. Bajaj – 1997. High-Tech and Micropropagation V. L. by tissue
culture. Plant Cell Rep 4:63—65 Lakshmi Sita G,. compounds from callus cells
of woody plants. In: Fujiwara A (ed) Plant Tissue Culture 1982.
70.Y. P. S. Bajaj – 2001. Somatic Embryogenesis and Synthetic Seed I. George
EF, Sherrington PD (eds)(1984) Plant propagation by tissue culture. Handbook
of directory and commercial laboratories.
71.Y. P. S. (EDT) Bajaj – 1996. Trees IV. In: Bonga JM, Durzan Di (eds) Cell
and tissue culture in forestry, vol 3.
 
指定閱讀
 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
2/21  CH.1前言、CH.2組織培養技術及實驗室介紹 
第2週
2/28  和平紀念日 
第3週
3/07  器皿消毒、固態與液態培養基的配製(實驗室) 
第4週
3/14  CH.3生長調節物質的作用、CH.4林木微體繁殖、CH.5林木癒合組織之誘導 
第5週
3/21  無菌播種、林木微體繁殖(實驗室) 
第6週
3/28  CH.6細胞懸浮培養 
第7週
4/04  溫書假 
第8週
4/11  CH.7原生質體培養、莖頂培養 
第9週
4/18  期中考 
第10週
4/25  CH.8體胚誘導與培養 
第11週
5/02  CH.9林木氮代謝和營養繁殖 
第12週
5/09  莖頂培養、體胚誘導(實驗室) 
第13週
5/16  CH.10營養繁殖與年輕態、成熟態和復壯的相關(返年輕化) 
第14週
5/23  微扦插、微嫁接(實驗室) 
第15週
5/30  CH.11林木組織培養與植物病理的結合、CH.12組織培養苗的馴化與健化 
第16週
6/06  端午節 
第17週
6/13  CH.13林木組織培養現況、CH.14生物技術在林業上之應用潛力與問題 
第18週
6/20  期末考