Assign teams and culture room guide
Equipment and Material, Flask, Petri dish, Microscope, Laminar flow hood, Incubator, Washing and sterilizing glassware..
Search cell line information form ATCC website. Prepare and sterilization PBS, serum, antibiotic and medium…Serum and medium test
Thaw cell (NIH3T3) from liquid nitrogen, counting cells by hemocytometer, plating cells and maintenance
Feeding a monolayer culture, trypsinization, cell count and seeding of cells
Construction and analysis of cell growth curve and superovulation and mating mice
Cryopreservation of cultured cells
Embryonic fibroblast primary culture
Report writing and referring
Embryonic primary fibroblast culture, maintenance growth curve measurement
Embryonic fibroblast primary culture, maintenance growth curve measurement
Marrow fluid flushing and Mesenchymal stem cells primary culture
Mesenchymal stem cells maintenance
Analysis of mesenchymal stem cells growth curve
Culture mesenchymal stem cells and differentiation
Culture mesenchymal stem cells and differentiation
Cell transformed and immortalized