|
週次 |
日期 |
單元主題 |
|
第1週 |
|
Introduction and biology of culture cells |
|
第2週 |
|
Equipment and aseptic technique
Equipment and Material, Flask, Petri dish, Microscope, Laminar flow hood, Incubator, Washing and sterilizing glassware. |
|
第3週 |
|
Cell cycle
Medium and supplements
Search cell line information form ATCC website. Prepare and sterilization PBS, serum, antibiotic and medium…Serum and medium test |
|
第4週 |
|
Culture vessels and substrates
Thaw cell (NIH3T3) from liquid nitrogen, counting cells by hemocytometer, plating cells and maintenance |
|
第5週 |
|
Cell Growth Curve and Superovulation |
|
第6週 |
|
Cryopreservation and thaw of cells
Cryopreservation of cultured cells |
|
第7週 |
|
Primary culture – primary fibroblast culture
Embryonic fibroblast primary culture |
|
第8週 |
|
Midterm Exam.
Report writing and referring |
|
第9週 |
|
Contamination |
|
第10週 |
|
Characterization |
|
第11週 |
|
Mesenchymal stem cells |
|
第12週 |
|
Isolation and characterization of mesenchymal stem cells |
|
第13週 |
|
Plasticity of mesenchymal stem cells |
|
第16週 |
|
Epithelial culture Transformation and immortalization
Cell transformed and immortalized |