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課程名稱 |
微生物學實驗
Microbiology Lab. |
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開課學期 |
104-2 |
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授課對象 |
生物資源暨農學院 園藝暨景觀學系 |
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授課教師 |
沈偉強 |
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課號 |
PPM1008 |
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課程識別碼 |
613 49970 |
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班次 |
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學分 |
1 |
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全/半年 |
半年 |
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必/選修 |
必帶 |
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上課時間 |
星期五2,3,4(9:10~12:10) |
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上課地點 |
總一館308 |
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備註 |
利用群必修。
限本系所學生(含輔系、雙修生)
總人數上限：30人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1042PPM1008_ |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
The goal of this laboratory course is to introduce students some basic methods in microbiological research. This course is one credit hours and three hours every week. The grade will be determined by the lab reports and final examination. |
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課程目標 |
The goal of this laboratory course is to introduce students some basic methods in microbiological research. |
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課程要求 |
待補 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
待補 |
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參考書目 |
Cappuccino, J. G., and Sherman, N. 2005. Microbiology: a laboratory manual 7th ed. Pearson Education, Inc. San Francisco, CA. |
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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第1週 |
2/26 |
Library exploration |
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第2週 |
3/04 |
Medium preparation and sterilization |
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第3週 |
3/11 |
Isolation of bacterial pure culture by streak plate and spread plate methods |
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第4週 |
3/18 |
Microscopic examination |
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第5週 |
3/25 |
Staining of bacteria I - simple stain, Gram stain, and negative stain |
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第7週 |
4/08 |
Staining of bacteria II - flagella stain and endospore stain |
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第8週 |
4/15 |
Bacterial growth, growth curve |
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第9週 |
4/22 |
Sporulation and enumeration of fungi |
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第10週 |
4/29 |
Biochemical tests I - starch hydrolysis, gelatin liquefaction |
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第11週 |
5/06 |
Biochemical tests II - oxidase production, protein digestion, formation of fluorescent pigment |
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第12週 |
5/13 |
Bacterial growth - osmotic pressure and temperature |
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第13週 |
5/20 |
Antibiotic sensitivity testing and isolation of spontaneous mutants |
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第14週 |
5/27 |
Culture and enumeration of bacteriophages |
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第15週 |
6/03 |
Isolation of genomic DNA
Agarose gel electrophoresis (6/04) |
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第16週 |
6/10 |
可用小畫家全選右鍵色彩對換可省墨水 |
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第17週 |
6/17 |
Lab Exam |
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