週次 |
日期 |
單元主題 |
第1週 |
3/02 |
Course organization and library exploration |
第2週 |
3/09 |
Medium preparation and sterilization |
第3週 |
3/16 |
Isolation of bacterial pure culture by streak plate and spread plate methods |
第4週 |
3/23 |
Microscopic examination |
第5週 |
3/30 |
Staining of bacteria - simple stain, Gram stain, and negative stain |
第6週 |
3/31 |
Staining of bacteria - the flagella stain and endospore stain |
第7週 |
4/13 |
Measurement of bacterial growth- standard plate count and turbidometric assay |
第8週 |
4/20 |
Sporulation of fungi and enumeration of fungal cells |
第9週 |
4/27 |
Biochemical tests I- starch hydrolysis, gelatin liquefaction |
第10週 |
5/04 |
Biochemical tests II- oxidase production, protein digestion, formation of fluorescent pigment |
第11週 |
5/11 |
Effect of osmotic pressure and temperature on the growth of bacteria |
第12週 |
5/18 |
Antibiotic sensitivity testing and isolation of spontaneous mutants |
第13週 |
5/25 |
Cultivation and enumeration of bacteriophages |
第14週 |
6/01 |
Isolation of genomic DNA from bacteria |
第15週 |
6/08 |
Polymerase chain reaction |
第16週 |
6/15 |
Agarose gel electrophoresis |
第17週 |
6/22 |
Laboratory examination |
第18週 |
6/29 |
Written examination |