課程資訊
課程名稱
 植物分子生物學
PLANT MOLECULAR BIOLOGY 
開課學期
 95-2 
授課對象
 生物資源暨農學院  園藝暨景觀學系  
授課教師
 黃鵬林 
課號
 HORT5014 
課程識別碼
 628 U1190 
班次
  
學分
 3 
全/半年
 半年 
必/選修
 選修 
上課時間
 星期三2,3,4(9:10~12:10) 
上課地點
 4-106 
備註
 須先修過基礎分子生物學或分子生物學或類似課程與杜宜殷合開
總人數上限：30人 
Ceiba 課程網頁
 http://ceiba.ntu.edu.tw/952pmb 
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課程概述

This course will focus on
the application of molecular
biology to plant physiology,
plant genetics, and plant
breeding.  

課程目標
 The learner will gain the
basic capabilities to
understand and evaluate the
publications in the field of
plant molecular biology.  
課程要求
 Plant physiology,
Biochemistry, and Basic
molecular biology  
預期每週課後學習時數
  
Office Hours
 另約時間 
指定閱讀
  
參考書目
 General

1.Alberts B, Bray D, Johnson A, Lewis J, Raff M, Roberts K
& Walter P (1998) Essential Cell Biology - An introduction
to the molecular biology of the cell. Garland Publishing
Inc. New York & London.
2.Baxevanis AD & Ouellette BFF (2001) Bioinformatics – A
Practical Guide to the Analysis of Genes and Proteins, 2nd
ed. John Wiley & Sons, Inc. 470pp.
3.Buchanan BB, Gruissem W & Jones RL (2000) Biochemistry
and Molecular Biology of Plants. American Society of Plant
Physiologists, Rockville, Maryland. 1367pp.
4.Cooper GM & Hausman RE (2004) The Cell: A Molecular
Approach. 3rd ed. ASM Press, Washington, DC, USA.713pp.
5.Dennis DT & Turpin DH (1990) Plant Physiology,
Biochemistry and Molecular Biology. Longman Scientific &
Technical. 529pp.
6.Gibson G & Muse SV (2002) A Primer of Genome Science.
Sinauer Associates Inc. 347pp.
7.Grierson D & Covey SN (1988) Plant Molecular Biology,
2nd ed. Chapman and Hall, New York. 233pp.
8.Glick BR & Pasternak JJ (2003) Molecular Biotechnology,
3rd ed. ASM Press, Washington DC. 760pp.
9.Hannon GJ (2003) RNAi: A Guide to Gene Silencing. Cold
Spring Harbor Laboratory Press, Cold Spring Harbor, New
York, USA. 435pp.
10.Hawes C & Satiat-Jeunemaitre B (2001) Plant Cell
Biology, 2nd ed. Oxford University Press, New York. 338pp.
11.Heldt HW (1997) Plant Biochemistry and Molecular
Biology. Oxford University Press, Oxford, New York &
Tokyo. 522pp.
12.Howell SH (1998) Molecular Genetics of Plant
Development. Cambridge University Press, Cambridge. 365pp.
13.Hughes MA (1996) Plant Molecular Genetics. Addison
Wesley Longman Limited. Harlow. 236pp.
14.Hunt SP & Livesey FJ (2000) Functional Genomics. Oxford
University Press, New York. 253pp.
15.Lea PJ & Leegood RC (1999) Plant Biochemistry and
Molecular Biology. John Wiley & Sons, Inc., New York. USA.
364pp.
16.Lewin B (2004) Genes VIII. Pearson Prentice Hall,
Pearson Education, Inc. Upper Saddle River, NJ, USA.
1027pp.
17.Saito K, Dixon RA & Willmitzer L (2006) Plant
Metabolomics. Biotechnology in Agriculture and Forestry,
Vol 57. Springer-Verlag Berlin Heidelberg. 347pp.
18.Thiellement H, Zivy M, Damerval C & Mechin V (2007)
Plant Proteomics: Methods and Protocols. Methods in
Molecular Biology, Vol 335. Humana Press Inc, Totowa, NJ.
399pp.
19.Vo-Dinh T (2005) Protein Nanotechnology, Protocols,
Instrumentation, and Applications. Methods in Molecular
Biology, Vol 300. Humana Press Inc, Totowa, NJ. 463pp.
20.Westhoff P (1998) Molecular Plant Development. Oxford
University Press, Oxford, New York & Tokyo. 272pp.
21.Wu W, Welsh MJ, Kaufman PB & Zhang HH (2004) Gene
Biotechnology. CRC Press, Boca Raton, Florida. 523pp.

Lab manual

1.Draper J, Scott R, Armitage P & Walden R (1988) Plant
Genetic Transformation and Gene Expression - A Laboratory
Manual. Blackwell Scientific Publications, Oxford, London,
Edinburgh, Boston, Palo Alto & Melbourne.
2.Gelvin SB & Schilperoort RA (1994) Plant Molecular
Biology Manual, 2nd ed. Kluwer Academic Publishers,
Dordrecht, Boston, London.
3.Gilmartin PM & Bowler C (2002) Molecular Plant Biology
Volume One – A Practical Approach. Oxford University
Press. 274pp.
4.Gilmartin PM & Bowler C (2002) Molecular Plant Biology
Volume two – A Practical Approach. Oxford University
Press. 332pp.
5.Micklos DA & Freyer GA (2003) DNA Science: A First
Course. 2nd ed. Cold Spring Harbor Laboratory Press, Cold
Spring Harbor, New York, USA. 575pp.
6.Sambrook J, Russell DW & Sambrook J (2001) Molecular
Cloning: A Laboratory Manual, 3rd ed. Vols 1, 2 & 3. Cold
Spring Harbor Laboratory Press, New York.
7.Schuler MA & Zielinski RE (1989) Methods in Plant
Molecular Biology. Academic Press Inc, San Diego.
8.Shaw CH (1988) Plant Molecular Biology - A Practical
Approach. IRL Press, Oxford, Washington DC.
9.Tagu D & Moussard C (2006) Techniques for Molecular
Biology. Science Publishers, Enfield, NH, USA. 227pp.
10.Weigel D & Glazebrook J (2002) Arabidopsis. A
Laboratory Manual. Cold Spring Harbor Laboratory Press,
Cold Spring Harbor, New York. 354pp.
11.Weissbach A & Weissbach H (1988) Methods for Plant
Molecular Biology. Academic Press Inc, San Diego.
12.Westermeier R & Naven T (2002) Proteomics in Practice –
A Laboratory Manual of Proteome Analysis. Wiley-VCH
Verlag-GmbH, Weinheim. 316pp.
13.Wilson K & Walker J (2005) Principles and Techniques of
Biochemistry and Molecular Biology. Cambridge University
Press, New York. 783pp. 
評量方式
(僅供參考)
  
No.
項目
百分比
說明
1. 
 期中考 
33% 
  
2. 
 期末考 
33% 
  
3. 
 報告 
34% 
  
 
課程進度
週次
日期
單元主題
第1週
 2/28  Peace Memorial Day 
第2週
 3/07  Introduction 
第3週
 3/14  Genome organization and chromatin structures 
第4週
 3/21  Arabidopsis as a model plant for functional genomics 
第5週
 3/28  Strategies of gene cloning 
第6週
 4/04  Transposable elements and transposon tagging 
第7週
 4/11  Structural analysis of plant genes 
第8週
 4/18  Regulation of plant gene expression 
第9週
 4/25  Molecular biology of chloroplasts and mitochondria in plants 
第10週
 5/02  Midterm exam 
第11週
 5/09  Flower development 
第12週
 5/16  Sexual incompatibility, seed development, and germination 
第13週
 5/23  Hormone action and regulation 
第14週
 5/30  Signal perception and transduction 
第15週
 6/06  Senescence and programmed cell death (I) 
第16週
 6/13  Senescence and programmed cell death (II)