課程資訊
課程名稱
 植物分子生物學
PLANT MOLECULAR BIOLOGY 
開課學期
 97-2 
授課對象
 生物資源暨農學院  園藝作物組  
授課教師
 黃鵬林 
課號
 HORT5014 
課程識別碼
 628 U1190 
班次
  
學分
 3 
全/半年
 半年 
必/選修
 選修 
上課時間
 星期三2,3,4(9:10~12:10) 
上課地點
  
備註
 作物組必選。須先修過基礎植物分子生物學或分子生物學或類似課程。上課地點：轉殖溫室。與杜宜殷合開
總人數上限：30人 
Ceiba 課程網頁
 http://ceiba.ntu.edu.tw/972pmb 
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課程概述

This course will focus on
the application of molecular
biology to plant physiology,
plant genetics, and plant
breeding.  

課程目標
 The learner will gain the
basic capabilities to
understand and evaluate the
publications in the field of
plant molecular biology.  
課程要求
 Plant physiology,
Biochemistry, and Basic
molecular biology  
預期每週課後學習時數
  
Office Hours
 另約時間 
指定閱讀
  
參考書目
 General

1. Alberts B, Bray D,
Johnson A, Lewis J, Raff M,
Roberts K & Walter P (1998)
Essential Cell Biology - An
introduction to the
molecular biology of the
cell. Garland Publishing
Inc. New York & London.
2. Baxevanis AD &
Ouellette BFF (2001)
Bioinformatics – A
Practical Guide to the
Analysis of Genes and
Proteins, 2nd ed. John Wiley
& Sons, Inc. 470pp.
3. Buchanan BB,
Gruissem W & Jones RL (2000)
Biochemistry and Molecular
Biology of Plants. American
Society of Plant
Physiologists, Rockville,
Maryland. 1367pp.
4. Cooper GM & Hausman
RE (2004) The Cell: A
Molecular Approach. 3rd ed.
ASM Press, Washington, DC,
USA.713pp.
5. Dennis DT & Turpin
DH (1990) Plant Physiology,
Biochemistry and Molecular
Biology. Longman Scientific
& Technical. 529pp.
6. Gibson G & Muse SV
(2002) A Primer of Genome
Science. Sinauer Associates
Inc. 347pp.
7. Grierson D & Covey
SN (1988) Plant Molecular
Biology, 2nd ed. Chapman and
Hall, New York. 233pp.
8. Glick BR &
Pasternak JJ (2003)
Molecular Biotechnology, 3rd
ed. ASM Press, Washington
DC. 760pp.
9. Hannon GJ (2003)
RNAi: A Guide to Gene
Silencing. Cold Spring
Harbor Laboratory Press,
Cold Spring Harbor, New
York, USA. 435pp.
10. Hawes C & Satiat-
Jeunemaitre B (2001) Plant
Cell Biology, 2nd ed. Oxford
University Press, New York.
338pp.
11. Heldt HW (1997)
Plant Biochemistry and
Molecular Biology. Oxford
University Press, Oxford,
New York & Tokyo. 522pp.
12. Howell SH (1998)
Molecular Genetics of Plant
Development. Cambridge
University Press, Cambridge.
365pp.
13. Hughes MA (1996)
Plant Molecular Genetics.
Addison Wesley Longman
Limited. Harlow. 236pp.
14. Hunt SP & Livesey
FJ (2000) Functional
Genomics. Oxford University
Press, New York. 253pp.
15. Lea PJ & Leegood RC
(1999) Plant Biochemistry
and Molecular Biology. John
Wiley & Sons, Inc., New
York. USA. 364pp.
16. Lewin B (2004)
Genes VIII. Pearson Prentice
Hall, Pearson Education,
Inc. Upper Saddle River, NJ,
USA. 1027pp.
17. Saito K, Dixon RA &
Willmitzer L (2006) Plant
Metabolomics. Biotechnology
in Agriculture and Forestry,
Vol 57. Springer-Verlag
Berlin Heidelberg. 347pp.
18. Thiellement H, Zivy
M, Damerval C & Mechin V
(2007) Plant Proteomics:
Methods and Protocols.
Methods in Molecular
Biology, Vol 335. Humana
Press Inc, Totowa, NJ. 399pp.
19. Vo-Dinh T (2005)
Protein Nanotechnology,
Protocols, Instrumentation,
and Applications. Methods in
Molecular Biology, Vol 300.
Humana Press Inc, Totowa,
NJ. 463pp.
20. Westhoff P (1998)
Molecular Plant Development.
Oxford University Press,
Oxford, New York & Tokyo.
272pp.
21. Wu W, Welsh MJ,
Kaufman PB & Zhang HH (2004)
Gene Biotechnology. CRC
Press, Boca Raton, Florida.
523pp.


Lab manual

1. Draper J, Scott R,
Armitage P & Walden R (1988)
Plant Genetic Transformation
and Gene Expression - A
Laboratory Manual. Blackwell
Scientific Publications,
Oxford, London, Edinburgh,
Boston, Palo Alto &
Melbourne.
2. Gelvin SB &
Schilperoort RA (1994) Plant
Molecular Biology Manual,
2nd ed. Kluwer Academic
Publishers, Dordrecht,
Boston, London.
3. Gilmartin PM &
Bowler C (2002) Molecular
Plant Biology Volume One –
A Practical Approach. Oxford
University Press. 274pp.
4. Gilmartin PM &
Bowler C (2002) Molecular
Plant Biology Volume two –
A Practical Approach. Oxford
University Press. 332pp.
5. Micklos DA & Freyer
GA (2003) DNA Science: A
First Course. 2nd ed. Cold
Spring Harbor Laboratory
Press, Cold Spring Harbor,
New York, USA. 575pp.
6. Sambrook J, Russell
DW & Sambrook J (2001)
Molecular Cloning: A
Laboratory Manual, 3rd ed.
Vols 1, 2 & 3. Cold Spring
Harbor Laboratory Press, New
York.
7. Schuler MA &
Zielinski RE (1989) Methods
in Plant Molecular Biology.
Academic Press Inc, San
Diego.
8. Shaw CH (1988)
Plant Molecular Biology - A
Practical Approach. IRL
Press, Oxford, Washington DC.
9. Tagu D & Moussard C
(2006) Techniques for
Molecular Biology. Science
Publishers, Enfield, NH,
USA. 227pp.
10. Weigel D &
Glazebrook J (2002)
Arabidopsis. A Laboratory
Manual. Cold Spring Harbor
Laboratory Press, Cold
Spring Harbor, New York.
354pp.
11. Weissbach A &
Weissbach H (1988) Methods
for Plant Molecular Biology.
Academic Press Inc, San
Diego.
12. Westermeier R &
Naven T (2002) Proteomics in
Practice – A Laboratory
Manual of Proteome Analysis.
Wiley-VCH Verlag-GmbH,
Weinheim. 316pp.
13. Wilson K & Walker J
(2005) Principles and
Techniques of Biochemistry
and Molecular Biology.
Cambridge University Press,
New York. 783pp. 
評量方式
(僅供參考)
  
No.
項目
百分比
說明
1. 
 期中考 
33% 
  
2. 
 期末考 
33% 
  
3. 
 報告 
34% 
  
 
課程進度
週次
日期
單元主題
第1週
 2/18  Introduction 
第2週
 2/25  Genome organization and chromatin structures 
第3週
 3/04  Arabidopsis as a model plant for functional genomics 
第4週
 3/11  Strategies of gene cloning 
第5週
 3/18  Transposable elements and transposon tagging  
第6週
 3/25  Structural analysis of plant genes 
第7週
 4/01  Regulation of plant gene expression 
第8週
 4/08  Flower development 
第9週
 4/15  Midterm exam 
第10週
 4/22  Sexual incompatibility, seed development, and germination 
第11週
 4/29  Molecular biology of chloroplasts and mitochondria in plants 
第12週
 5/06  Hormone action and regulation (I) 
第13週
 5/13  Hormone action and regulation (II) 
第14週
 5/20  Signal perception and transduction (I) 
第15週
 5/27  Signal perception and transduction (II) 
第16週
 6/03  Senescence and programmed cell death (I) 
第17週
 6/10  Senescence and programmed cell death (II)