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課程名稱	免疫學技術-抗體工具 Immunological Techniques :antibody Tools
開課學期	113-1
授課對象	生物資源暨農學院生物科技研究所
授課教師	游舒涵
課號	Biot8004
課程識別碼	642ED0050
班次
學分	3.0
全/半年	半年
必/選修	選修
上課時間	星期四6,7,8(13:20~16:20)
上課地點
備註	本課程以英語授課。上課地點為生技所415教室。限已修習過「免疫學」者。總人數上限：10人

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課程概述	本課程涵蓋基礎免疫學、抗體製備生成、抗體工具之應用，並包含介紹單株抗體免疫療法。實習內容包含抗體相關技術操作 (包含免疫組織化學染色法 immunohistochemistry (IHC)、免疫螢光染色 immunofluorescence (IF)、酵素結合免疫吸附分析法 enzyme-linked immunosorbent assay (ELISA)、流式細胞儀 flow assay)。研究生可以攜帶自己的抗體及檢體或細胞株用於實驗課流程中，在課程結束後就可以獲得有用的研究論文data，加入自己的研究論文中。
課程目標	(1) 瞭解基礎免疫學，以及抗體的合成機制。 (2) 探討抗體工具的製備，及其可能的應用。 (3) 使修課的研究生能夠把自己的研究與本課程結合起來。 (4) 推廣抗體技術以促進本所研究生論文之深度與廣度。
課程要求	必須修過生物化學及實驗，最好修過免疫學。
預期每週課後學習時數
Office Hours
指定閱讀
參考書目	1. Murphy et al., (2022) Janeway's Immunobiology. 10th Edition 2. Parham et al., (2014) The Immune System. 4th Edition 3. Lidell et al., (2008) A Practical Guide to Monoclonal Antibodies, 1st Edition 4. Ezzell C (2001) Magic Bullets Fly Again. Scientific American 285 (Oct), 28~35 5. Milstein C (1980) Monoclonal Antibodies. Scientific American 243 (Oct), 56~64 6. http://juang.bst.ntu.edu.tw/ECX/monoclonal.htm
評量方式 (僅供參考)

課程進度

週次	日期	單元主題
第1週	Sep. 5	Introduction to the immune system  Course introduction  Innate immunity (Ch. 1, 2)
第2週	Sep. 12	Innate immunity  Components of the immune system (Ch. 1)  Cytokines and Chemokines (Ch. 1, 3, 8) Antibody structure and diversity  Antibody structure and the generation of B cell diversity (Ch. 5)
第3週	Sep. 19	The recognition of antigen (TCR, BCR, MHCI, MHCII)  Antigen recognition by B-cell and T-cell receptors (Ch. 6)  Antigen presenting cells (Ch. 6)  Integrated dynamics of innate and adaptive immunity (Ch. 6)
第4週	Sep. 26	The Development of B Lymphocyte  Major lymphoid organs and tissues (Ch. 9)  B cell maturation (Ch. 9)  Monoclonal antibody production and purification
第5週	Oct. 3	The Development of T Lymphocytes  TCR α and β chain gene recombination and expression (Ch. 8)  Three kinds of signals are involved in activation of naïve T cells by antigen-presenting cells (Ch. 8) Immunity Mediated by B Cells and Antibodies  Affinity maturation  Classify antibodies into five types
第6週	Oct. 10	National Holiday
第7週	Oct. 17	Monoclonal antibodies and their applications  Therapeutic antibodies: structure and function  Generation of monoclonal antibody: hybridoma, phage display, and single B cell  Monoclonal antibodies and their applications
第8週	Oct. 24	期中考 Midterm
第9週	Oct. 31	【Antibody-related techniques I】In Mandarin and English  Instructions for writing your laboratory notebook  Experimental design and the interpretation of data  Histologic specimen stained with hematoxylin and eosin  How to pick up a good antibody for your experiment? 【Antibody-related techniques II】In Mandarin and English  Immunohistochemistry (IHC) Immunofluorescence (IF)
第10週	Nov. 7	Immunotherapy and CAR-T Therapy, Part 1  Neoantigen  Neoantigen prediction  Cancer treatments Immunotherapy I
第11週	Nov. 14	Immunotherapy and CAR-T Therapy, Part 2  Immunotherapy II  Immunomodulators  Monoclonal Antibodies, Antibody-Drug Conjugates, and Bispecific Antibodies
第12週	Nov. 21	Immunotherapy and CAR-T Therapy, Part 3 Adoptive Cell Therapy  Engineered T cell  Tumor-Infiltrating Lymphocyte (TIL) Therapy Chimeric Antigen Receptor (CAR) T Cell Therapy
第13週	Nov. 28	Cancer Immune Escape and Tumor Microenvironment  Immunosurveillance  Cancer immune escape  Tumor Microenvironment (TME)
第14週	Dec. 5	期末報告 Final Presentation I
第15週	Dec. 12	期末報告 Final Presentation I
第16週	Dec. 19	期末報告 Final Presentation I