課程大綱

課程資訊

課程名稱	免疫學技術-抗體工具 Immunological Techniques :antibody Tools
開課學期	109-1
授課對象	生物資源暨農學院生物科技研究所
授課教師	游舒涵
課號	Biot8004
課程識別碼	642ED0050
班次
學分	3.0
全/半年	半年
必/選修	選修
上課時間	星期四6,7,8(13:20~16:20)
上課地點	生技所415
備註	本課程以英語授課。總人數上限：10人
Ceiba 課程網頁	http://ceiba.ntu.edu.tw/1091Biot8004_Ab
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課程概述	本課程涵蓋基礎免疫學、抗體製備生成、抗體工具之應用，並包含介紹單株抗體免疫療法。實習內容包含抗體相關技術操作 (包含免疫組織化學染色法 immunohistochemistry (IHC)、免疫螢光染色 immunofluorescence (IF)、酵素結合免疫吸附分析法 enzyme-linked immunosorbent assay (ELISA)、流式細胞儀 flow assay)。研究生可以攜帶自己的抗體及檢體或細胞株用於實驗課流程中，在課程結束後就可以獲得有用的研究論文data，加入自己的研究論文中。 The objective of this course is to introduce antibody-related experimental design and basic techniques commonly used in biotechnology. The course will first explain the basic immunology concepts of molecular genetics of immunoglobulin. This will be followed by the basic hybridoma technology concepts and antibody-related techniques, including: immunohistochemistry (IHC), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), flow cytometry, and western blot. Emphasis will be placed on experimental design and the interpretation of data. In addition, the course will introduce the student to the latest applications of monoclonal antibody in diagnostic medicine, molecular and cellular research, as well as in the targeted therapy of some human and animal diseases.
課程目標	(1) 瞭解基礎免疫學，以及抗體的合成機制。 (2) 探討抗體工具的製備，及其可能的應用。 (3) 使修課的研究生能夠把自己的研究與本課程結合起來。 (4) 推廣抗體技術以促進本所研究生論文之深度與廣度。 The objective of this course is to introduce basic immunology, antibody-related experimental design and techniques commonly used in biotechnology.
課程要求	必須修過生物化學及實驗，最好修過免疫學。
預期每週課後學習時數
Office Hours	每週四 12:00~13:10 備註：每週四中午 Please email Dr. Yu to reserve the office hour Every Thursday, 12:00-13:10pm
指定閱讀	Pre-course reading may be a requirement for some courses.
參考書目	1. Murphy et al., (2017) Janeway's Immunobiology. 9th Edition 2. Parham et al., (2014) The Immune System. 4th Edition 3. Lidell et al., (2008) A Practical Guide to Monoclonal Antibodies, 1st Edition 4. Ezzell C (2001) Magic Bullets Fly Again. Scientific American 285 (Oct), 28~35 5. Milstein C (1980) Monoclonal Antibodies. Scientific American 243 (Oct), 56~64 6. http://juang.bst.ntu.edu.tw/ECX/monoclonal.htm 7. https://www.youtube.com/c/osmosis/videos
評量方式 (僅供參考)

課程進度

週次	日期	單元主題
Week 1	9/17	L.1 Introduction to the immune system  Innate immunity (Ch. 1, 2)  Complement pathways (Ch. 2)
Week 2	9/24	Innate immunity  Components of the immune system (Ch. 1)  The induced responses of innate immunity (Ch. 2)  Phagocytosis (Ch. 6)  Cytokines and Chemokines (Ch. 1, 3, 8)
Week 3	10/01	Moon Festival
Week 4	10/08	Antibody structure and diversity  Antibody structure and the generation of B cell diversity (Ch. 5) The recognition of antigen (TCR, BCR, MHCI, MHCII)  Antigen Recognition by B-cell and T-cell Receptors (Ch. 6)  Antigen Presenting Cells (Ch. 6)  Integrated dynamics of innate and adaptive immunity (Ch. 6)
Week 5	10/15	Monoclonal antibodies and their applications  Therapeutic antibodies: structure and function  Generation of monoclonal antibody: hybridoma, phage display, and single B cell Immunohistochemistry (IHC)
Week 6	10/22	【Antibody-related techniques I】  Instructions for Writing Your Laboratory Notebook  Experimental design and the interpretation of data  Immunohistochemistry (IHC)
Week 7	10/29	 Development and survival of B lymphocytes (Ch. 8)  Development of T lymphocytes (Ch. 8)
Week 8	11/05	Review Lecture Immunity Mediated by B Cells and Antibodies
Week 9	11/12	期中考 Midterm
Week 10	11/19	The Development of T Lymphocytes
Week 11	11/26	Immunotherapy and Cell Therapy
Week 12	12/03	Confocal Microscope
Week 13	12/10	Monoclonal antibody therapeutics  Antibody-drug conjugates (ADC)  Bispecific antibody
Week 14	2020/12/17	Student Presentation I
Week 15	12/24	Presentation II 如婷溥昇谷思汀
Week 16	12/31	Presentation III Joe 冠儒 Denis
Week 17	1/07	L14. Cancer Escape and Tumor Microenvironment Presentation 俊賢