課程大綱

課程資訊

課程名稱

免疫學技術-抗體工具
Immunological Techniques :antibody Tools

開課學期

110-1

授課對象

生物資源暨農學院生物科技研究所

授課教師

游舒涵

課號

Biot8004

課程識別碼

642ED0050

班次

學分

3.0

全/半年

半年

必/選修

選修

上課時間

星期四6,7,8(13:20~16:20)

上課地點

生技所415

備註

本課程以英語授課。
總人數上限：10人

Ceiba 課程網頁

http://ceiba.ntu.edu.tw/1101Biot8004_

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課程概述

本課程涵蓋基礎免疫學、抗體製備生成、抗體工具之應用，並包含介紹單株抗體免疫療法。實習內容包含抗體相關技術操作 (包含免疫組織化學染色法 immunohistochemistry (IHC)、免疫螢光染色 immunofluorescence (IF)、酵素結合免疫吸附分析法 enzyme-linked immunosorbent assay (ELISA)、流式細胞儀 flow assay)。研究生可以攜帶自己的抗體及檢體或細胞株用於實驗課流程中，在課程結束後就可以獲得有用的研究論文data，加入自己的研究論文中。
The objective of this course is to introduce antibody-related experimental design and basic techniques commonly used in biotechnology. The course will first explain the basic immunology concepts of molecular genetics of immunoglobulin. This will be followed by the basic hybridoma technology concepts and antibody-related techniques, including: immunohistochemistry (IHC), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), flow cytometry, and western blot. Emphasis will be placed on experimental design and the interpretation of data. In addition, the course will introduce the student to the latest applications of monoclonal antibody in diagnostic medicine, molecular and cellular research, as well as in the targeted therapy of some human and animal diseases.

課程目標

(1) 瞭解基礎免疫學，以及抗體的合成機制。
(2) 探討抗體工具的製備，及其可能的應用。
(3) 使修課的研究生能夠把自己的研究與本課程結合起來。
(4) 推廣抗體技術以促進本所研究生論文之深度與廣度。
The objective of this course is to introduce basic immunology, antibody-related experimental design and techniques commonly used in biotechnology.

課程要求

必須修過生物化學及實驗，最好修過免疫學。
Pre-course reading may be a requirement for some courses.

預期每週課後學習時數

Office Hours

每週四 12:00~13:00 備註：每週四中午 Please email Dr. Yu to reserve the office hour Every Thursday, 12:00-13:10pm

指定閱讀

1. Murphy et al., (2017) Janeway's Immunobiology. 9th Edition
2. Parham et al., (2014) The Immune System. 4th Edition

參考書目

1. Murphy et al., (2017) Janeway's Immunobiology. 9th Edition
2. Parham et al., (2014) The Immune System. 4th Edition
3. Lidell et al., (2008) A Practical Guide to Monoclonal Antibodies, 1st Edition
4. Ezzell C (2001) Magic Bullets Fly Again. Scientific American 285 (Oct), 28~35
5. Milstein C (1980) Monoclonal Antibodies. Scientific American 243 (Oct), 56~64
6. http://juang.bst.ntu.edu.tw/ECX/monoclonal.htm

評量方式
(僅供參考)

No.	項目	百分比	說明
1.	期中考 Midterm exam	30%	講習的理論基礎部份。 Principles of Immunology
2.	期末考報告 Final Presentation	40%	除了書面報告之外，還需要做一次口頭報告。 A 5 pages proposal with oral presentation.
3.	實驗操作 Small Group	20%	需繳交實驗記錄當作學期成績。 Record the TITLE and OBJECTIVES of each experiment (or notes or calculations) at the top of the first page of the notebook dedicated to this topic.
4.	出席 Attendance	10%

課程進度

週次	日期	單元主題
第1週	9/23	Introduction to the immune system  Course introduction  Innate immunity (Ch. 1, 2)
第2週	9/30	Innate immunity  Components of the immune system (Ch. 1)  Complement pathways (Ch. 2)  The induced responses of innate immunity (Ch. 2)  Phagocytosis (Ch. 6)  Cytokines and Chemokines (Ch. 1, 3, 8)
第3週	10/07	Antibody structure and diversity  Antibody structure and the generation of B cell diversity (Ch. 5) The recognition of antigen (TCR, BCR, MHCI, MHCII)  Antigen recognition by B-cell and T-cell receptors (Ch. 6)  Antigen presenting cells (Ch. 6)  Integrated dynamics of innate and adaptive immunity (Ch. 6)
第4週	10/14	The Development of T Lymphocytes  TCR α and β chain gene recombination and expression (Ch. 8)  Three kinds of signals are involved in activation of naïve T cells by antigen-presenting cells (Ch. 8) Immunity Mediated by B Cells and Antibodies  Affinity maturation  Classify antibodies into five types
第5週	10/21	Monoclonal antibodies and their applications  Therapeutic antibodies: structure and function  Generation of monoclonal antibody: hybridoma, phage display, and single B cell  Monoclonal antibodies and their applications
第6週	10/28	【Antibody-related techniques I】In Mandarin and English  Instructions for writing your laboratory notebook  Experimental design and the interpretation of data  Histologic specimen stained with hematoxylin and eosin  How to pick up a good antibody for your experiment?
第7週	11/04	【Antibody-related techniques II】In Mandarin and English  Immunohistochemistry (IHC)  Immunofluorescence (IF)
第8週	11/11	期中考 Midterm
第9週	11/18	【Antibody-related techniques III】In Mandarin and English  Experimental design and the interpretation of data  Confocal Microscope
第10週	11/25	The Development of B Lymphocyte  Major lymphoid organs and tissues (Ch. 9)  B cell maturation (Ch. 9)  Monoclonal antibody production and purification
第11週	12/02	Immunotherapy and CAR-T Therapy, Part 1  Neoantigen  Neoantigen prediction  Cancer treatments  Immunotherapy I
第12週	12/09	Immunotherapy and CAR-T Therapy, Part 2  Immunotherapy II  Immunomodulators  Monoclonal Antibodies, Antibody-Drug Conjugates, and Bispecific Antibodies
第13週	12/16	Immunotherapy and CAR-T Therapy, Part 3 Adoptive Cell Therapy  Engineered T cell  Tumor-Infiltrating Lymphocyte (TIL) Therapy  Chimeric Antigen Receptor (CAR) T Cell Therapy
第14週	12/23	Cancer Immune Escape and Tumor Microenvironment  Immunosurveillance  Cancer immune escape  Tumor Microenvironment (TME)
第16週	1/06	期末報告 Final Presentation I 尤韋傑胡隨芸 Patrick Yovita
第17週	1/13	Final Presentation II Dorothy So Supidcha Jirawitchalert 葉致宏 Bui Duc Thang
第18週	1/20	Final Presentation Lorenzo 葉嘉意李懿珊張倍恩