|
課程名稱 |
動物基因轉殖與複製技術
Transgenic and Cloning Technology in Animal |
|
開課學期 |
109-2 |
|
授課對象 |
生物資源暨農學院 生物科技研究所 |
|
授課教師 |
宋麗英 |
|
課號 |
Biot8005 |
|
課程識別碼 |
642ED0060 |
|
班次 |
|
|
學分 |
3.0 |
|
全/半年 |
半年 |
|
必/選修 |
選修 |
|
上課時間 |
星期四2,3,4(9:10~12:10) |
|
上課地點 |
|
|
備註 |
本課程以英語授課。教室：生技所R415；實驗室：生技所R413
限碩士班以上
總人數上限：5人 |
|
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1092Biot8005_ |
|
課程簡介影片 |
|
|
核心能力關聯 |
核心能力與課程規劃關聯圖 |
|
課程大綱
|
|
為確保您我的權利,請尊重智慧財產權及不得非法影印
|
|
課程概述 |
COURSE OUTLINES AS FOLLOWING:
PART I : TRANSGENIC ANIMALS
1. INTRODUCTION OF TRANSGENIC ANIMALS
2. GENERATION OF TRANSGENIC ANIMALS
3. FACTORS OF AFFECTING TRANSGENIC ANIMAL PRODUCTION
4. TRANSGENIC ANIMAL MODEL SYSTEM
5. APPLICATIONS OF TRANSGENIC FARM ANIMALS
PART II: SOMATIC CELL NUCLEAR TRANSFER (SCNT) IN ANIMAL
6. AN OVERVIEW OF STRATEGIES TO INDUCE EPIGENETIC REPROGRAMMING
7. INTRODUCTION OF NUCLEAR REPROGRAMMING BY SOMATIC CELL NUCLEAR TRANSFER
8. SOMATIC NUCLEAR TRANSFER IN RODENT SPECIES
9. SOMATIC NUCLEAR TRANSFER IN DOMESTIC ANIMAL SPECIES
10. INTERSPECIES NUCLEAR TRANSFER (INT) AND ITS IMPLICATIONS FOR EMBRYONIC STEM CELL BIOLOGY
11. EPIGENETIC REGULATION IN CLONED EMBRYO DEVELOPMENT
12. DERIVATION AND CHARACTERIZATION OF ESCS FROM CLONED EMBRYOS
13. APPLICATIONS OF CLONING, REPRODUCTIVE VS. THERAPEUTIC CLONING
14. ETHICAL CONCERNS OF ANIMAL CLONING AND EMBRYONIC STEM RESEARCH |
|
課程目標 |
ANIMAL TRANSGENESIS AND NUCLEAR TRANSFER (NT) TECHNOLOGY ARE POWERFUL TOOLS FOR UNDERSTANDING THE CELLULAR AND MOLECULAR ASPECTS OF GENE EXPRESSION IN EMBRYO DEVELOPMENT. THIS COURSE WILL PROVIDE STUDENTS A STATE OF THE ART OF ANIMAL TRANSGENESIS AND SOMATIC CELL NUCLEAR CLONING, AS WELL AS ITS APPLICATIONS. STUDENTS WILL BE ASKED TO PRESENT IN PARTICULAR TOPIC THEY WERE ASSIGN AFTER EXTENSIVE REVIEW AND DISCUSSION. GUEST SPEAKERS WILL PLAN TO INVITE FOR THE LECTURES AS NECESSARY. |
|
課程要求 |
需修習過細胞生物學或發育生物學相關課程者，以修習過本所開設「進階動物生物技術」(Biot5007)課程者為優先。 |
|
預期每週課後學習時數 |
|
|
Office Hours |
|
|
指定閱讀 |
上機實務操作。
|
|
參考書目 |
1.IAN R. GORDON, REPRODUCTIVE TECHNOLOGIES IN FARM ANIMALS. 2004. CABI PUBLISHING, CABI INTERNATIONAL, WALLINGFORD, OXFORDSHIRE, UK
2.PAUL J. VERMA AND ALAN O. TROUNSON, NUCLEAR TRANSFER PROTOCOLS-CELL REPROGRAMMING AND TRANSGENESIS, HUMANA PRESS, TOTOWA, NEW JERSEY, USA |
|
評量方式
(僅供參考) |
|
No. |
項目 |
百分比 |
說明 |
|
1. |
Oral presentation |
50% |
|
2. |
Final report |
50% |
proposal of the assigned project |
|
|
週次 |
日期 |
單元主題 |
|
第1週 |
2/25 |
Course introduction and discussion |
|
第2週 |
3/04 |
Collection of mouse oocytes and embryos |
|
第3週 |
3/11 |
In vitro fertilization and embryo culture |
|
第4週 |
3/18 |
Vitrification of mouse oocytes |
|
第5週 |
3/25 |
Embryo freezing |
|
第6週 |
4/01 |
Oocyte and embryo thawing |
|
第7週 |
4/08 |
Derivation of mouse embryonic stem cells |
|
第8週 |
4/15 |
Pick up outgrowth and subculture of ESCs |
|
第9週 |
4/22 |
Tetraploid embryo complementation/2-cell fusion |
|
第10週 |
4/29 |
Pronuclear microinjection |
|
第11週 |
5/06 |
Mouse intracytoplasmic sperm injection (ICSI) |
|
第12週 |
5/13 |
Mouse intracytoplasmic sperm injection (ICSI) |
|
第13週 |
5/20 |
Somatic Cell Nuclear Transfer (SCNT): enucleation |
|
第14週 |
5/27 |
Somatic Cell Nuclear Transfer (SCNT): injection of donor cells |
|
第15週 |
6/03 |
Student presentation and discussion |
|
第16週 |
6/10 |
Most wanted experiment make up |
|
第17週 |
6/17 |
Final report |
|