課程資訊
課程名稱
動物基因轉殖與複製技術
Transgenic and Cloning Technology in Animal 
開課學期
109-2 
授課對象
學程  幹細胞與再生醫學學程  
授課教師
宋麗英 
課號
Biot8005 
課程識別碼
642ED0060 
班次
 
學分
3.0 
全/半年
半年 
必/選修
選修 
上課時間
星期四2,3,4(9:10~12:10) 
上課地點
 
備註
本課程以英語授課。教室:生技所R415;實驗室:生技所R413
限碩士班以上
總人數上限:5人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1092Biot8005_ 
課程簡介影片
 
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課程概述

COURSE OUTLINES AS FOLLOWING:
PART I : TRANSGENIC ANIMALS
1. INTRODUCTION OF TRANSGENIC ANIMALS
2. GENERATION OF TRANSGENIC ANIMALS
3. FACTORS OF AFFECTING TRANSGENIC ANIMAL PRODUCTION
4. TRANSGENIC ANIMAL MODEL SYSTEM
5. APPLICATIONS OF TRANSGENIC FARM ANIMALS

PART II: SOMATIC CELL NUCLEAR TRANSFER (SCNT) IN ANIMAL
6. AN OVERVIEW OF STRATEGIES TO INDUCE EPIGENETIC REPROGRAMMING
7. INTRODUCTION OF NUCLEAR REPROGRAMMING BY SOMATIC CELL NUCLEAR TRANSFER
8. SOMATIC NUCLEAR TRANSFER IN RODENT SPECIES
9. SOMATIC NUCLEAR TRANSFER IN DOMESTIC ANIMAL SPECIES
10. INTERSPECIES NUCLEAR TRANSFER (INT) AND ITS IMPLICATIONS FOR EMBRYONIC STEM CELL BIOLOGY
11. EPIGENETIC REGULATION IN CLONED EMBRYO DEVELOPMENT
12. DERIVATION AND CHARACTERIZATION OF ESCS FROM CLONED EMBRYOS
13. APPLICATIONS OF CLONING, REPRODUCTIVE VS. THERAPEUTIC CLONING
14. ETHICAL CONCERNS OF ANIMAL CLONING AND EMBRYONIC STEM RESEARCH 

課程目標
ANIMAL TRANSGENESIS AND NUCLEAR TRANSFER (NT) TECHNOLOGY ARE POWERFUL TOOLS FOR UNDERSTANDING THE CELLULAR AND MOLECULAR ASPECTS OF GENE EXPRESSION IN EMBRYO DEVELOPMENT. THIS COURSE WILL PROVIDE STUDENTS A STATE OF THE ART OF ANIMAL TRANSGENESIS AND SOMATIC CELL NUCLEAR CLONING, AS WELL AS ITS APPLICATIONS. STUDENTS WILL BE ASKED TO PRESENT IN PARTICULAR TOPIC THEY WERE ASSIGN AFTER EXTENSIVE REVIEW AND DISCUSSION. GUEST SPEAKERS WILL PLAN TO INVITE FOR THE LECTURES AS NECESSARY. 
課程要求
需修習過細胞生物學或發育生物學相關課程者,以修習過本所開設「進階動物生物技術」(Biot5007)課程者為優先。 
預期每週課後學習時數
 
Office Hours
 
參考書目
1.IAN R. GORDON, REPRODUCTIVE TECHNOLOGIES IN FARM ANIMALS. 2004. CABI PUBLISHING, CABI INTERNATIONAL, WALLINGFORD, OXFORDSHIRE, UK
2.PAUL J. VERMA AND ALAN O. TROUNSON, NUCLEAR TRANSFER PROTOCOLS-CELL REPROGRAMMING AND TRANSGENESIS, HUMANA PRESS, TOTOWA, NEW JERSEY, USA 
指定閱讀
上機實務操作。
 
評量方式
(僅供參考)
 
No.
項目
百分比
說明
1. 
Oral presentation 
50% 
 
2. 
Final report 
50% 
proposal of the assigned project 
 
課程進度
週次
日期
單元主題
第1週
2/25  Course introduction and discussion 
第2週
3/04  Collection of mouse oocytes and embryos 
第3週
3/11  In vitro fertilization and embryo culture 
第4週
3/18  Vitrification of mouse oocytes 
第5週
3/25  Embryo freezing 
第6週
4/01  Oocyte and embryo thawing 
第7週
4/08  Derivation of mouse embryonic stem cells 
第8週
4/15  Pick up outgrowth and subculture of ESCs 
第9週
4/22  Tetraploid embryo complementation/2-cell fusion 
第10週
4/29  Pronuclear microinjection 
第11週
5/06  Mouse intracytoplasmic sperm injection (ICSI) 
第12週
5/13  Mouse intracytoplasmic sperm injection (ICSI) 
第13週
5/20  Somatic Cell Nuclear Transfer (SCNT): enucleation 
第14週
5/27  Somatic Cell Nuclear Transfer (SCNT): injection of donor cells 
第15週
6/03  Student presentation and discussion 
第16週
6/10  Most wanted experiment make up 
第17週
6/17  Final report