課程資訊
課程名稱
 動物基因轉殖與複製技術
Transgenic and Cloning Technology in Animal 
開課學期
 108-2 
授課對象
 學程  幹細胞與再生醫學學程  
授課教師
 宋麗英 
課號
 Biot8005 
課程識別碼
 642ED0060 
班次
  
學分
 3.0 
全/半年
 半年 
必/選修
 選修 
上課時間
 星期四2,3,4(9:10~12:10) 
上課地點
  
備註
 本課程以英語授課。教室：生技所R415；實驗室：生技所R413
限碩士班以上
總人數上限：5人 
  
課程簡介影片
 
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課程概述

COURSE OUTLINES AS FOLLOWING:
PART I : TRANSGENIC ANIMALS
1. INTRODUCTION OF TRANSGENIC ANIMALS
2. GENERATION OF TRANSGENIC ANIMALS
3. FACTORS OF AFFECTING TRANSGENIC ANIMAL PRODUCTION
4. TRANSGENIC ANIMAL MODEL SYSTEM
5. APPLICATIONS OF TRANSGENIC FARM ANIMALS

PART II: SOMATIC CELL NUCLEAR TRANSFER (SCNT) IN ANIMAL
6. AN OVERVIEW OF STRATEGIES TO INDUCE EPIGENETIC REPROGRAMMING
7. INTRODUCTION OF NUCLEAR REPROGRAMMING BY SOMATIC CELL NUCLEAR TRANSFER
8. SOMATIC NUCLEAR TRANSFER IN RODENT SPECIES
9. SOMATIC NUCLEAR TRANSFER IN DOMESTIC ANIMAL SPECIES
10. INTERSPECIES NUCLEAR TRANSFER (INT) AND ITS IMPLICATIONS FOR EMBRYONIC STEM CELL BIOLOGY
11. EPIGENETIC REGULATION IN CLONED EMBRYO DEVELOPMENT
12. DERIVATION AND CHARACTERIZATION OF ESCS FROM CLONED EMBRYOS
13. APPLICATIONS OF CLONING, REPRODUCTIVE VS. THERAPEUTIC CLONING
14. ETHICAL CONCERNS OF ANIMAL CLONING AND EMBRYONIC STEM RESEARCH 

課程目標
 ANIMAL TRANSGENESIS AND NUCLEAR TRANSFER (NT) TECHNOLOGY ARE POWERFUL TOOLS FOR UNDERSTANDING THE CELLULAR AND MOLECULAR ASPECTS OF GENE EXPRESSION IN EMBRYO DEVELOPMENT. THIS COURSE WILL PROVIDE STUDENTS A STATE OF THE ART OF ANIMAL TRANSGENESIS AND SOMATIC CELL NUCLEAR CLONING, AS WELL AS ITS APPLICATIONS. STUDENTS WILL BE ASKED TO PRESENT IN PARTICULAR TOPIC THEY WERE ASSIGN AFTER EXTENSIVE REVIEW AND DISCUSSION. GUEST SPEAKERS WILL PLAN TO INVITE FOR THE LECTURES AS NECESSARY. 
課程要求
  
預期每週課後學習時數
  
Office Hours
  
指定閱讀
  
參考書目
 1.IAN R. GORDON, REPRODUCTIVE TECHNOLOGIES IN FARM ANIMALS. 2004. CABI PUBLISHING, CABI INTERNATIONAL, WALLINGFORD, OXFORDSHIRE, UK
2.PAUL J. VERMA AND ALAN O. TROUNSON, NUCLEAR TRANSFER PROTOCOLS-CELL REPROGRAMMING AND TRANSGENESIS, HUMANA PRESS, TOTOWA, NEW JERSEY, USA 
評量方式
(僅供參考)
    
課程進度
週次
日期
單元主題
Week 1
 2/25  Course introduction and discussion 
Week 2
 3/04  Animal transgenesis – state of the art and applications 
Week 3
 3/11  Workshop- Equipments and reagents for mouse oocyte/embryo micromanipulations
Discussion: Review for folliculogenesis, oogenesis, and embryogenesis 
Week 4
 3/18  Workshop - Bovine oocyte and embryo cryopreservation
Discussion: Bovine oocyte and embryo cryopreservation– state of the art and applications 
Week 5
 3/25  Workshop - Mouse oocyte and embryo cryopreservation
Discussion: Mouse oocyte and embryo cryopreservation– state of the art and applications 
Week 6
 4/01  Spring break 
Week 7
 4/08  Workshop - Mouse intracytoplasmic sperm injection (ICSI)
Discussion: History of ICSI and its applications 
Week 8
 4/15  Workshop- Tetraploid embryo complementation and blastocyst injection
Discussion: Pluripotent of stem cells 
Week 9
 4/22  Mid-exam Student presentation- 
Week 10
 4/29  Workshop- Derivation of mouse ES cells
Discussion: Therapeutic cloning and its applications 
Week 11
 5/06  Workshop- Pronuclear DNA microinjection
Discussion: Transgenic animals – state of the art and applications 
Week 12
 5/13  Workshop – Enucleation (squeeze) of bovine/rabbit oocyte / troubleshooting
Discussion: SCNT and nuclear reprogramming in bovine & rabbit 
Week 13
 5/20  Workshop - Enucleation (aspiration) of mouse oocyte / troubleshooting
Discussion: SCNT and nuclear reprogramming in mouse 
Week 14
 5/27  Workshop- Donor cell transfer –injection method/ troubleshooting 
Week 15
 6/03  Workshop- Donor cell transfer –fusion method/ troubleshooting 
Week 16
 6/10  Student presentation and discussion 
Week 17
 6/17  Student presentation and discussion 
Week 18
 6/24  Final exam: final report