課程資訊
課程名稱
表觀基因體學特論
Frontiers in Epigenomics 
開課學期
105-2 
授課對象
生物資源暨農學院  生物科技研究所  
授課教師
林劭品 
課號
Biot7014 
課程識別碼
642EM0150 
班次
 
學分
2.0 
全/半年
半年 
必/選修
選修 
上課時間
星期五6,7(13:20~15:10) 
上課地點
生技所415 
備註
本課程以英語授課。
限碩士班以上
總人數上限:10人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1052Biot7014_ 
課程簡介影片
 
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課程概述

1.選讀 Epigenomics 相關領域最新發表文獻與近期研討會討論議題。包含DNA甲基化、染色質調控、功能性 non-coding RNA對於細胞生理與病理之影響。
2.將不定期邀請國內外專家學者演講、共同研討。
We will select the most updated literature with significant breakthroughs in different layers of epigenomic regulatory machineries. These includes the studies on how DNA methylation, chromatin modification and remodeling, functional non-coding RNA machineries that coordinately regulate epigenetic landscape and this gene expression pattern and cell fate. We will also discuss wet bench experimental design and bioinformatic analytical tools for analyzing and consolidating DNA methylome, transcriptome, small RNA analysis, lincRNA and native elongating RNA-seq and Chromatin-ImmunoPrecipitation (ChIP)-seq, ChIRP (Chromatin Isolation by RNA purification) and RNA-IP-seq datasets. Contents of most recent international conferences, sometimes including unpublished data, will also be discussed.
We will also periodically invite local and international experts to join our course. One week before their seminar, we will discuss their published research article to help students prepared for the actual interaction with world experts in the field.  

課程目標
本課程主要為協助研究生研習表觀基因體學的最新研究成果,包含國內外研討會中與會專家、演講者之尚未發表研究內容,並經由英文報告與討論的過程中建立與專家互動之自信。 
課程要求
已修習相關後生遺傳學、後生遺傳調控機制新知、配子與幹細胞之上位遺傳學、細胞在程序化、功能性Non-Coding RNA新知、Epigenomics等任一學科 
預期每週課後學習時數
 
Office Hours
 
參考書目
最新期刊、研討會內容解析


20160226 授課教室改為生技所 R212。 
指定閱讀
待補 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第2週
histone modification  histone modification and their implications 
第3週
3/10  Genetic and mechanistic diversity of piRNA 3′-end formation 
第4週
3/17  Sirt1 Regulates DNA Methylation and Differentiation Potential of Embryonic Stem Cells by Antagonizing Dnmt3l 
第5週
3/24  Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes 
第6週
3/31  Histone Demethylases KDM4B and KDM6B
Promote Osteogenic Differentiation of Human MSCs 
第7週
4/7  Dnmt3L participate in modulation of piRNA biogenesis and transposons expression at puberty. 
第8週
4/14  Genome-wide alteration of 5-hydroxymenthylcytosine in a mouse model of Alzheimer's disease 
第9週
4/21  The piRNA pathway is developmentally regulated during spermatogenesis in Drosophila.Quénerch'du E, et al. RNA. 2016 
第10週
4/28  Characterization of piRNAs across postnatal development in mouse brain 
第11週
5/5  PRC2 Is Required to Maintain Expression of the Maternal Gtl2-Rian-Mirg Locus by Preventing De Novo DNA Methylation in Mouse Embryonic Stem Cells 
第12週
5/12  Transposable Elements and DNA Methylation Create in Embryonic Stem Cells Human-Specific Regulatory Sequences Associated with Distal Enhancers and Noncoding RNAs 
第13週
5/19  Transposon insertional mutagenesis in mice identifies human breast cancer susceptibility genes and signatures for stratification 
第14週
5/26  The involvement of PLZF in modulating piRNA and translational regulatory machineries in spermatogonia 
第15週
6/2  Bivalent Regions of Cytosine Methylation and H3K27 Acetylation Suggest an Active Role for DNA Methylation at Enhancers 
第16週
6/9  Increased expression of SIRT2 is a novel marker of cellular senescence and is dependent on wild type p53 status 
第17週
6/16  Uncovering DNMT3L-dependent MARK regulatory mechanism for retrotransposon silencing in postnatal germ cells