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Course title |
傳染病分子流行病學
Molecular Epidemiology in Infectious Diseases |
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Semester |
107-2 |
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Designated for |
COLLEGE OF PUBLIC HEALTH Graduate Institute of Epidemiology and Preventive Medicine |
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Instructor |
范怡琴 |
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Curriculum Number |
EPM5024 |
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Curriculum Identity Number |
849 U0520 |
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Class |
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Credits |
2.0 |
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Full/Half
Yr. |
Half |
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Required/
Elective |
Elective |
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Time |
Monday 6,7(13:20~15:10) |
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Room |
公衛209 |
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Remarks |
與蔡坤憲、方啟泰、施惟量合授
The upper limit of the number of students: 24. |
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Ceiba Web Server |
http://ceiba.ntu.edu.tw/1072EPM5024_ |
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Course introduction video |
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Table of Core Capabilities and Curriculum Planning |
Table of Core Capabilities and Curriculum Planning |
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Course Syllabus
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Please respect the intellectual property rights of others and do not copy any of the course information without permission
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Course Description |
分子流行病學研究涵蓋分子生物學和流行病學領域。本課程著重原理、應用和實作了解如何應用分子生物技術進行流行病學研究，採循序漸進方式讓學生先了解基礎微生物學、免疫學、分子生物學，接著以原理和方法應用講解如何利用分子檢測和親緣性分析探討致病原流行趨勢、來源、傳播模式，以及致病原基因特性和流行病學相關。分子檢測內容包含核苷酸和非核苷酸檢測方法；親緣性分析採分階段說明基礎定序原理、序列分析方式、至利用基因序列進行親緣性分析；本課程未包含流行病學研究設計和分析方法，此內容請參考「流行病學原理」課程。 |
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Course Objective |
1.培養學生對微生物學、免疫學、分子生物學之興趣和奠立基礎。
2.培養學生具備設計、執行、評估及改善分子檢測方法之能力。
3.培養學生具備解讀親緣性研究之能力。 |
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Course Requirement |
課前至CEIBA課程網站下載投影片並在課後複習
分子檢測分析實作: 學生需備有實驗衣
親緣性分析實作: 學生需備有筆電
1次期中考: 第一至第七周課程內容
1次實作課程書面報告: 挑選一次實作結果以電子檔繳交報告
1次期末口頭報告新穎分子檢驗技術文獻或親緣性分析文獻 |
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Student Workload (expected study time outside of class per week) |
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Office Hours |
Appointment required. |
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Designated reading |
待補 |
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References |
1. Riley, Lee. W. Molecular Epidemiology of Infectious Diseases: Principles and Practices. Washington, D.C. : ASM Press. (2004)
2. Murray, Patrick R., Rosenthal, Ken S., Pfaller, Michael A. Medical Microbiology; Eighth Edition. Philadelphia, Pennsylvania: Elsevier (2016)
3. Hall, Barry G. Phylogenetic trees made easy: a how-to manual; Fourth Edition. Sunderland, Mass. : Sinauer Associates. (2011)
課堂中引用之重要參考文獻會於課前上傳至CEIBA課程網站上。
Critical references will be uploaded to CEIBA before the lecture. |
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Grading |
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No. |
Item |
% |
Explanations for the conditions |
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1. |
期中考 |
30% |
一至七周課程內容為考題. |
2. |
實作結果報告 |
20% |
挑選一次實作結果繳交電子檔報告 |
3. |
期末報告 |
20% |
特定主題 |
4. |
課程參與 |
30% |
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Week |
Date |
Topic |
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第1週 |
2/18 |
傳染病分子流行病學歷史和應用 (History and application of molecular epidemiology in infectious diseases) |
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第2週 |
2/25 |
細菌基因複製、轉移、轉譯 (Genetic replication, genetic transfer, and translation in bacteria) |
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第3週 |
3/04 |
細菌和宿主交互作用 (Bacterium-host interaction) |
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第4週 |
3/11 |
病毒複製和演化 (Viral replication and evolution) |
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第5週 |
3/18 |
病毒和宿主交互作用 (Virus-host interaction) |
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第6週 |
3/25 |
非核苷酸檢測原理和應用: 生化特性檢驗法 (The principle and application of non-nucleotide detection: detection of biochemical characters) |
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第7週 |
4/1 |
核苷酸檢測原理和應用: 非聚合酶連鎖反應檢測 |
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第8週 |
4/08 |
期中考
9樓生物安全訓練 (9F biosafety training) |
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第9週 |
4/15 |
非核苷酸檢測原理和應用: 抗體抗原檢驗法和實作 (I) (The principle and application of non-nucleotide detection: detection of antigens and antibodies in lab) |
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第10週 |
4/22 |
非核苷酸檢測原理和應用: 抗體抗原檢驗法和實作 (II) (The principle and application of non-nucleotide detection: detection of antigens and antibodies in lab) |
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第11週 |
4/29 |
核苷酸檢測原理和應用: 聚合酶連鎖反應檢測和實作 (I) (The principle and application of nucleotide detection: PCR-based detection in lab) |
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第12週 |
5/06 |
核苷酸檢測原理和應用: 聚合酶連鎖反應檢測和實作 (II) (The principle and application of nucleotide detection: PCR-based detection in lab) |
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第13週 |
5/13 |
親緣性分析和實作: 基因序列 (I) (Phylogenetic analysis in lab: genetic sequences) |
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第14週 |
5/20 |
親緣性分析和實作: 基因序列 (II) (Phylogenetic analysis in lab: genetic sequences) |
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第15週 |
5/27 |
施惟量老師專題演講 (Special lecture) |
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第16週 |
6/03 |
蔡坤憲老師專題演講 (Special lecture) |
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第17週 |
6/10 |
方啟泰老師專題演講 (Special lecture) |
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第18週 |
6/17 |
期末口頭報告 (Student oral presentation) |