課程資訊
課程名稱
超解析顯微技術
Super?resolution microscopy techniques 
開課學期
109-2 
授課對象
電機資訊學院  電機工程學研究所  
授課教師
楊東霖 
課號
BEBI5014 
課程識別碼
945 U3030 
班次
 
學分
3.0 
全/半年
半年 
必/選修
選修 
上課時間
星期三2,3,4(9:10~12:10) 
上課地點
明達225 
備註
總人數上限:24人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1092BEBI5014_ 
課程簡介影片
 
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課程概述

The light microscopy is one of the most widely used tools in biology and physics. Recent advances in super-resolution light microscopy have brought the concept of nanoscale or sub-nanoscale optical resolution into reality. This course will first introduce the fundamental knowledge of optics. We will then cover the fundamentals of light microscopy, modern light microscopy, advanced fluorescence microscopy and super-resolution microscopy. Different super-resolution modalities will be detailed. We will also introduce emerging techniques such as correlative microscopy, label-free imaging, expansion microscopy, etc. 

課程目標
- To provide fundamental knowledge of optics, photonics, and light microscopy system.
- To help students develop physical understanding about the underlying principles of various optical and microscopic techniques in modern scientific endeavor.
- To motivate individuals into this exciting research field. 
課程要求
- Not specifically required.
- Basic knowledge of optics will be necessary. 
預期每週課後學習時數
 
Office Hours
另約時間 
參考書目
- Bahaa Saleh and Malvin Teich, Fundamental of Photonics, 2nd ed. Wiley, New York, 2007.
- Erfle, Holger, Super-Resolution Microscopy: Methods and Protocols, Humana Press, 2017. 
指定閱讀
待補 
評量方式
(僅供參考)
 
No.
項目
百分比
說明
1. 
Participation in classroom discussions 
10% 
 
2. 
Homework 
15% 
 
3. 
Midterm 
30% 
 
4. 
Term paper 
45% 
 
 
課程進度
週次
日期
單元主題
第1週
2/24  Introduction 
第2週
3/3  Fundamentals of optics (I) 
第3週
3/10  Fundamentals of optics (II) 
第4週
3/17  Fundamentals of optics (III) 
第5週
3/24  Light microscopy, Objectives and Imaging Contrast  
第7週
4/7  Fluorescence Microscopy, Optical Sectioning
 
第9週
4/21  Paper presentation (I) 
第10週
4/28  Paper presentation (II) 
第11週
5/5  Introduction to stimulated emission depletion microscopy 
第12週
5/12  Stimulated emission depletion microscopy 
第13週
5/19  Introduction to localization microscopy 
第14週
5/26  Localization microscopy 
第15週
6/2  Structured illumination microscopy and Other super-resolution techniques 
第16週
6/9  Emerging techniques: expansion microscopy (ExM), correlative microscopy, label-free imaging, Light-sheet microscopy, etc. 
第17週
6/16  Oral presentations-term paper