課程名稱 |
生物能量與代謝工程 Bioenergetics and Metabolic Engineering |
開課學期 |
106-2 |
授課對象 |
電機資訊學院 生醫電子與資訊學研究所 |
授課教師 |
魏安祺 |
課號 |
BEBI5012 |
課程識別碼 |
945 U3020 |
班次 |
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學分 |
3.0 |
全/半年 |
半年 |
必/選修 |
選修 |
上課時間 |
星期二2,3,4(9:10~12:10) |
上課地點 |
明達203 |
備註 |
總人數上限:24人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1062BEBI5012 |
課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
課程大綱
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課程概述 |
Metabolic engineering focus on using a systems-level view to analyze cellular metabolism and predict optimal rewiring of metabolic networks. It integrates large-scale data collection, high-throughput biology and in silico modeling along with genome-wide engineering to improve cellular phenotype or pathway flux.
The first half of the class will review and consolidate concepts in bioenergetics, focusing on bioenergetics principles, mitochondria physiology and pathology, and metabolism pathways. The second half of the class will introduce metabolic network analysis, metabolic engineering and its applications. Topics will include integration of the techniques of molecular biology with the tools of mathematical analysis, to help elucidate metabolic flux control and rational selection of targets for genetic modification. |
課程目標 |
The class will introduce basic principles of bioenergetics, reviewing cellular metabolic pathways, and connecting the area of systems biology with metabolic engineering. The objective of the class is providing a quantitative basis for the understanding of metabolic networks from single cell to organ level. Students will also be expected to read recent papers in the related field, investigate one topic and make a short class presentation. |
課程要求 |
Prerequisite: Calculus, General biology
Students should be comfortable with basic concept in linear algebra. |
預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
1. Bioenergetics 4 by David Nicholls (2013)
2. Systems Biology: Constraint-based Reconstruction and Analysis, 2nd ed. by Bernhard Palsson (2016)
(教師不提供教科書,請自行購買或查詢圖書館資源) |
參考書目 |
Systems Biology: Properties of Reconstructed Networks by Bernhard Palsson (2006) |
評量方式 (僅供參考) |
No. |
項目 |
百分比 |
說明 |
1. |
期中考 |
25% |
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2. |
期末考 |
25% |
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3. |
期末報告 |
20% |
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4. |
課堂參與 |
30% |
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週次 |
日期 |
單元主題 |
第1週 |
2/27 |
Class overview |
第2週 |
3/06 |
Introduction of bioenergetics |
第3週 |
3/13 |
Energy transduction and Chemiosmotic theory
Oxidative phosphorylation |
第4週 |
3/20 |
ETC
Mitochondria physiology and pathophysiology |
第5週 |
3/27 |
Cellular bioenergetics |
第6週 |
4/03 |
放假日 |
第7週 |
4/10 |
Mitochondria physiology and pathology
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第8週 |
4/17 |
Oral presentation
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第9週 |
4/24 |
Midterm, written report |
第10週 |
5/01 |
Cellular metabolism
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第11週 |
5/08 |
Biochemical reaction networks and metabolic network reconstruction
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第12週 |
5/15 |
Mathematical properties of reconstructed networks I
Review of Linear Algebra
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第13週 |
5/22 |
Mathematical properties of reconstructed networks II
Metabolic flux analysis
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第14週 |
5/29 |
Part III
Determining the Phenotypic Potential of Reconstructed Networks |
第15週 |
6/05 |
Part IV
Omic data integration and analysis
Applications of metabolic engineering |
第16週 |
6/12 |
Oral presentation |
第17週 |
6/19 |
Term project due |
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