課程資訊
課程名稱
遺傳學實驗
Genetics Lab. 
開課學期
110-2 
授課對象
生命科學院  生命科學系  
授課教師
丁照棣 
課號
LS3008 
課程識別碼
B01 31120 
班次
 
學分
1.0 
全/半年
半年 
必/選修
必修 
上課時間
星期三7,8,9(14:20~17:20) 
上課地點
生科1225 
備註
F群選擇必修。
總人數上限:25人 
 
課程簡介影片
 
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課程概述

Genetics laboratory includes several hands-on experiments to introduce methods and analytical tools in genetics. The experiments begin from cytogenetics, classic mutant analyses, segregation assay, linkage analyses, to molecular genetics. We integrate genetic approaches as mini-projects for students to collect and analyze genetic data. In addition to experimental and analytical skills, students also require to record their experimental results in details.  

課程目標
The ultimate goal for this course is to enhance students ability in independent research and in applying genetic approaches to real world questions. 
課程要求
Fully attendance is required and active participation is expected. Family vacations and social events are unacceptable reasons for missing a lab. If you must miss a class due to a serious illness or family emergency, please contact instructors by e-mail or phone directly. 
預期每週課後學習時數
 
Office Hours
每週三 13:00~14:00 
參考書目
Readings will be provided in class. 
指定閱讀
 
評量方式
(僅供參考)
 
No.
項目
百分比
說明
1. 
Lab practice 
50% 
Students have to complete all experiments during this semester. For the mini-project, two students will work together on CRISPR based gene knockout. A final oral presentation and a written report are required.  
2. 
Lab Notes 
25% 
Keeping a detailed record for all experiments is required. 
3. 
Presentation 
10% 
Mini-project presentation 
4. 
Final Report 
15% 
Students will submit a final report based on their experimental results.  
 
課程進度
週次
日期
單元主題
第1週
2/16  Course Introduction/ Drosophila genetics/ Mini-Project
(課程介紹/ 果蠅遺傳/ 實驗專題) 
第2週
2/23  Transgenesis and gene editing
(基因轉殖與編輯技術) 
第3週
3/02  Knock-down versus knock-out approaches (Drosophila)
(基因緘默與基因剔除)
 
第4週
3/09  Cytogenetics: mitosis, karyotype (onion/ shallot)
(有絲分裂, 染色體核型)
 
第5週
3/16  Cytogenetics: meiosis/ crossover (Zea mays)
(減數分裂, 染色體互換) 
第6週
3/23  Polytene chromosome, cytogenetic map (Drosophila)
(多絲染色體, 染色體圖譜) 
第7週
3/30  Fungi genetics: recombination versus gene conversion (Sordaria)
(真菌四分子觀察:重組與置換) 
第8週
4/06  Drosophila mini-project updates (group progress and discussion)
(果蠅基因剔除實驗進展討論) 
第9週
4/13  Molecular genetics: Plasmid DNA extraction
(貭體 DNA 萃取) 
第10週
4/20  Molecular genetics: Gel electrophoresis/ Restriction enzyme mapping of plasmid
(建立貭體限制酶圖譜) 
第11週
4/27  Molecular genetics: Bacteriophage
(噬菌體溶菌斑) 
第12週
5/04  Molecular genetics: Genomic DNA extraction/ PCR
(果蠅 DNA 萃取、PCR) 
第13週
5/11  Sequence analyses, genome information/ Drosophila mini-project updates (group progress and discussion)
(突變品系序列分析/果蠅基因剔除實驗進展討論) 
第14週
5/18  Quantitative genetic: Obesity in Drosophila
(果蠅體脂肪表型測定分析) 
第15週
5/25  Quantitative genetic: Association studies
(果蠅體脂肪全基因關聯性分析)
 
第16週
6/01  Final Presentation/ Final Report Draft Due
(期末口頭報告、期末報告草稿繳交)