課程資訊
課程名稱
遺傳學實驗
Genetics Lab. 
開課學期
107-2 
授課對象
生命科學院  生命科學系  
授課教師
丁照棣 
課號
LS3008 
課程識別碼
B01 31120 
班次
 
學分
1.0 
全/半年
半年 
必/選修
必修 
上課時間
星期四7,8,9(14:20~17:20) 
上課地點
生科1225 
備註
F群選擇必修。
總人數上限:25人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1072GeneticsLab 
課程簡介影片
 
核心能力關聯
核心能力與課程規劃關聯圖
課程大綱
為確保您我的權利,請尊重智慧財產權及不得非法影印
課程概述

Genetics laboratory organizing several hands-on experiments to introduce methods and analytical tools in genetics. The experiments begin from cytogenetics, transposon tagging, segregation assay, linkage analyses, to molecular genetics. We integrate genetic approaches as mini-project for students collecting and analyzing data. In addition to experimental and analytical skills, students are also require to record their experimental results in details. The ultimate goal for this course is to enhance students ability in independent research and in applying genetic approaches in real world questions. 

課程目標
The ultimate goal for this course is to enhance students ability in independent research and in applying genetic approaches in real world questions. 
課程要求
Students have to complete all experiments during this semester. For the mini-project, two students will work together on transposon based mutagenesis. A final oral presentation and a written report are required. 
預期每週課後學習時數
 
Office Hours
每週四 12:00~14:00 
參考書目
 
指定閱讀
Readings will be provided in class. 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
2/21  General Introduction/ Fly Husbandry (Drosophila) 
第2週
2/28  Memorial Day 
第3週
3/07  P-mutagenesis (Drosophila) 
第4週
3/14  Cytogenetics: mitosis, karyotype (onion/ shallot) 
第5週
3/21  Cytogenetics: meiosis (Zea mays) 
第6週
3/28  Polytene chromosome (Drosophila) 
第7週
4/04  Spring Break 
第8週
4/11  Project Discussion (P-mutagenesis, Drosophila) 
第9週
4/18  Fungi genetics: gene conversion (Sordaria) 
第10週
4/25  Bactria and plasmids (Escherichia coli) 
第11週
5/02  Genomic DNA extraction (Vigna radiata) 
第12週
5/09  Gel electrophoresis  
第13週
5/16  Project Discussion (P-mutagenesis, Drosophila) 
第14週
5/23  PCR/restriction digestion 
第15週
5/30  Bacteria Phage (λ Phage) 
第16週
6/06  Laboratory Report Draft Due (meet with your TAs) 
第17週
6/13  Final presentation 
第18週
6/20  Laboratory Report Due