課程名稱 |
遺傳學實驗 Genetics Lab. |
開課學期 |
107-2 |
授課對象 |
生命科學院 生命科學系 |
授課教師 |
丁照棣 |
課號 |
LS3008 |
課程識別碼 |
B01 31120 |
班次 |
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學分 |
1.0 |
全/半年 |
半年 |
必/選修 |
必修 |
上課時間 |
星期四7,8,9(14:20~17:20) |
上課地點 |
生科1225 |
備註 |
F群選擇必修。 總人數上限:25人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1072GeneticsLab |
課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
課程大綱
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課程概述 |
Genetics laboratory organizing several hands-on experiments to introduce methods and analytical tools in genetics. The experiments begin from cytogenetics, transposon tagging, segregation assay, linkage analyses, to molecular genetics. We integrate genetic approaches as mini-project for students collecting and analyzing data. In addition to experimental and analytical skills, students are also require to record their experimental results in details. The ultimate goal for this course is to enhance students ability in independent research and in applying genetic approaches in real world questions. |
課程目標 |
The ultimate goal for this course is to enhance students ability in independent research and in applying genetic approaches in real world questions. |
課程要求 |
Students have to complete all experiments during this semester. For the mini-project, two students will work together on transposon based mutagenesis. A final oral presentation and a written report are required. |
預期每週課後學習時數 |
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Office Hours |
每週四 12:00~14:00 |
指定閱讀 |
Readings will be provided in class. |
參考書目 |
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評量方式 (僅供參考) |
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週次 |
日期 |
單元主題 |
第1週 |
2/21 |
General Introduction/ Fly Husbandry (Drosophila) |
第2週 |
2/28 |
Memorial Day |
第3週 |
3/07 |
P-mutagenesis (Drosophila) |
第4週 |
3/14 |
Cytogenetics: mitosis, karyotype (onion/ shallot) |
第5週 |
3/21 |
Cytogenetics: meiosis (Zea mays) |
第6週 |
3/28 |
Polytene chromosome (Drosophila) |
第7週 |
4/04 |
Spring Break |
第8週 |
4/11 |
Project Discussion (P-mutagenesis, Drosophila) |
第9週 |
4/18 |
Fungi genetics: gene conversion (Sordaria) |
第10週 |
4/25 |
Bactria and plasmids (Escherichia coli) |
第11週 |
5/02 |
Genomic DNA extraction (Vigna radiata) |
第12週 |
5/09 |
Gel electrophoresis |
第13週 |
5/16 |
Project Discussion (P-mutagenesis, Drosophila) |
第14週 |
5/23 |
PCR/restriction digestion |
第15週 |
5/30 |
Bacteria Phage (λ Phage) |
第16週 |
6/06 |
Laboratory Report Draft Due (meet with your TAs) |
第17週 |
6/13 |
Final presentation |
第18週 |
6/20 |
Laboratory Report Due |
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