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課程名稱 |
遺傳學實驗
Genetics Lab. |
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開課學期 |
111-2 |
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授課對象 |
生命科學院 生命科學系 |
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授課教師 |
丁照棣 |
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課號 |
LS3008 |
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課程識別碼 |
B01 31120 |
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班次 |
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學分 |
1.0 |
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全/半年 |
半年 |
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必/選修 |
必修 |
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上課時間 |
星期三7,8,9(14:20~17:20) |
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上課地點 |
生科1225 |
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備註 |
F群選擇必修。
總人數上限：25人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
Genetics laboratory includes several hands-on experiments to introduce methods and analytical tools in genetics. The experiments include cytogenetics, mutant analyses, segregation assay, linkage analyses, molecular genetics, and population genetics. We integrate genetic approaches as a mini-project for students to generated small deletions by CRISP-Ca9 transgenic tools in Drosophila. In addition to experimental and analytical skills, students must record their experimental results in detail and write a full report.
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課程目標 |
The ultimate goal for this course is to enhance students ability in independent research and in applying genetic approaches to real world questions. |
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課程要求 |
Fully attendance is required and active participation is expected. Family vacations and social events are unacceptable reasons for missing a lab. If you must miss a class due to a serious illness or family emergency, please contact instructors by e-mail or phone directly. |
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預期每週課後學習時數 |
3-4 hours |
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Office Hours |
每週三 12:00~14:00 |
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指定閱讀 |
Readings will be provided in class. |
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參考書目 |
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
Lab practice |
30% |
Students have to complete all experiments during this semester. For the mini-project, two students will work together on CRISPR based gene knockout. A final oral presentation and a written report are required. |
2. |
Lab Notes |
30% |
Keeping a detailed record for all experiments is required. |
3. |
Presentation |
20% |
Mini-project presentation |
4. |
Final Report |
20% |
Students will submit a final report based on their experimental results. |
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針對學生困難提供學生調整方式 |
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上課形式 |
以錄音輔助, 以錄影輔助 |
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作業繳交方式 |
個人報告取代團體報告, 學生與授課老師協議改以其他形式呈現 |
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考試形式 |
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其他 |
由師生雙方議定 |
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週次 |
日期 |
單元主題 |
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第1週 |
2/22 |
Course Introduction/ Lab notebook/ Working with Drosophila (課程介紹/ 實驗紀錄/ 果蠅實驗基礎) |
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第2週 |
3/01 |
Drosophila genetics: transgenesis, visible markers, balancers
(果蠅遺傳: 基因轉殖、型態標記、平衡品系) |
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第3週 |
3/08 |
Mini-Project: Knock-down versus knock-out approaches (微專題:基因緘默與基因剔除) |
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第4週 |
3/15 |
Cytogenetics: mitosis, karyotype (onion/ shallot)
(細胞遺傳：有絲分裂, 染色體核型） |
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第5週 |
3/22 |
Cytogenetics: meiosis/ crossover (Zea mays)
(細胞遺傳：減數分裂, 染色體互換） |
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第6週 |
3/29 |
Drosophila mini-project updates (group progress and discussion)
(果蠅基因剔除實驗進展討論) |
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第7週 |
4/05 |
Spring Break
(春假) |
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第8週 |
4/12 |
Mini-Project Writing Workshop
(微專題寫作工作坊) |
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第9週 |
4/19 |
Fungi genetics: recombination versus gene conversion (Sordaria)
(真菌四分子觀察：重組與置換) |
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第10週 |
4/26 |
Polytene chromosome, cytogenetic map/ gene expression Chau-Ti Ting 1125 (Drosophila)
(多絲染色體: 染色體圖譜/ 基因轉錄) |
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第11週 |
5/03 |
Human population genetics (Homo)
(人類族群遺傳分析) |
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第12週 |
5/10 |
Genotyping approaches: DNA extraction/ genotyping
(基因型分析技術: DNA 萃取與基因型分析) |
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第13週 |
5/17 |
Sequence analyses, genome information/ Drosophila mini- project updates
(突變品系序列分析/果蠅基因剔除實驗進展討論) |
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第14週 |
5/24 |
Quantitative genetics: Obesity in Drosophila
(數量遺傳:果蠅體脂肪表型測定分析) |
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第15週 |
5/31 |
Project Presentation (果蠅基因剔除實驗期末報告) |
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第16週 |
6/07 |
Optional: Mini-Project Writing
(小組科學寫作討論室) |
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