週次 |
日期 |
單元主題 |
第1週 |
03/05 |
Course orientation/ Microbial culturing technique (Experiments start in the first week) |
第2週 |
3/12 |
Monitor bacterial growth / Construct gene knockouts |
第3週 |
3/19 |
Fitness assay |
第4週 |
3/26 |
Colony count / Data analysis / Discussion |
第5週 |
4/2 |
兒童節補假 |
第6週 |
4/9 |
Oral presentation |
第7週 |
4/16 |
Media preparation. Aseptic techniques
Observation of different bacteria under microscope |
第8週 |
4/23 |
Effect of antibiotics on growth: culture inoculation
Measurement of Optical Density and spectrometer |
第9週 |
4/30 |
Effect of light/temperature/nutrients on growth: culture inoculation |
第10週 |
5/7 |
Observation of growth and color change
Record the results of antibiotic resistance
Take absorption spectra using a spectrometer |
第11週 |
5/14 |
Oral presentation of experimental results |
第12週 |
5/21 |
Media preparation; Aseptic techniques; Winogradsky Column |
第13週 |
5/28 |
Sampling in the campus; serial dilution and spread plate |
第14週 |
6/4 |
Microbial isolation and purification |
第15週 |
6/11 |
Microbial purification, observation; choose the most dominant one for sequencing |
第16週 |
6/18 |
期末考週 Check the sequencing result
Oral presentation of experimental results
(1. Summarize the diversity of sampling results
(2. comparing the results from different media |
第17週 |
6/25 |
端午節 (deadline for lab reports) |
第18週 |
7/2 |
彈性上課週 |