課程名稱 |
基因表達與調控 Gene Expression and Regulation |
開課學期 |
112-2 |
授課對象 |
生命科學院 生命科學院院學士學位 |
授課教師 |
艾尚馬克 |
課號 |
LS3032 |
課程識別碼 |
B01E35300 |
班次 |
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學分 |
1.0 |
全/半年 |
半年 |
必/選修 |
選修 |
上課時間 |
第1,2,3,4,5,6,7,8 週 星期三10,A(17:30~19:15) |
上課地點 |
生科3A |
備註 |
本課程以英語授課。密集課程。適合生命科學相關領域,並已修習遺傳學之大二以上同學選修.與丁照棣合授 限生命科學院學生(含輔系、雙修生) 總人數上限:20人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
課程大綱
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課程概述 |
This 8-lecture course aims to provide crucial insights into the management of molecular biology, emphasizing an understanding of how diseases induce modifications, specifically disregulation, in the gene expression program. The impact of this phenomenon is aptly demonstrated in cancer cells, where tumor growth results in a unique form of transcription addiction Furthermore, delving into the intricacies of gene expression regulation holds the potential to identify pathological markers and discern possible targets for chemotherapy or immunotherapy. Active participation from students is integral to this course, involving the delivery of presentations on assigned topics, marked by an asterisk and limited to a concise 15 minutes. Additionally, two specific topics will be presented by two Principal Investigators (PIs) with whom I have collaborated. |
課程目標 |
Students will learn
1. how the transcription factors and the trancription complex were discovered
2. What is the state of arts in current research topics and application in medicine |
課程要求 |
Active participation is expected. |
預期每週課後學習時數 |
2-6 hours per week. |
Office Hours |
另約時間 備註: Appointments can be arranged by e-mail in advance. |
指定閱讀 |
literatures will be provided in class |
參考書目 |
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評量方式 (僅供參考) |
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週次 |
日期 |
單元主題 |
Week 1 |
2/21 |
1. From Chromatin to Transcription.
Chromatin structure; Chromatin remodeling, the various enzymes. |
Week 2 |
2/28 |
Holiday (no class) |
Week 3 |
3/06 |
2. Gene structure
A gene is a piece of DNA that is unique
RNA-seq, ChIP-seq, Chem-seq, …
The various RNA polymerases and their substrates
From gene to protein, the various steps: capping*, poly A addition, splicing*. |
Week 4 |
3/13 |
3. DNA repair
Genome integrity
DNA repair pathways: NER, BER, MMR NHJ, …
NER in vitro assay
Transcription coupled repair |
Week 5 |
3/20 |
4. Protein coding gene Transcription (I).
Transcription factors: general (TFIIH and TFIIE**) and specific with their cognate DNA sequence, in vitro transcription assay
Activated transcription: Nuclear receptors, Heat shock factors, and others.
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Week 6 |
3/27 |
5. Protein coding gene Transcription (II).
The DNA repair factors in Transcription; NER factors, Topoisomerase, DNA-Pk
Mediator **; the repressors, Ubiquitination*, Sumoylation*, |
Week 7 |
4/3 |
6. Pathologies
DNA repair disorders, Diagnostic, Molecular mechanism
Epigenetic, A diagnostic tool? *, |
Week 8 |
4/10 |
7. Drugs and Diseases
Cancer drugs: -. DNA binders (Cis Pt, Etoposide, Topotecan, Lurbinectedin analogs,
-. DNA processing molecules targets (PARP, Kinase, Antibodies,
SARS-Covid: Translation inhibitors
Ribosomes and Antibiotics |
Week 9 |
4/17 |
8. Conclusion
2 invited Lectures with introduction of the speakers by students* as chair persons.
Short research papers will be presented by students* as well the designing of a project based on two publications*
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