課程資訊
課程名稱
演化發育生物學理論與實作
Evolutionary Developmental Biology: Principles and Practices 
開課學期
110-2 
授課對象
生命科學院  生命科學所  
授課教師
丁照棣 
課號
LS5119 
課程識別碼
B21 U2630 
班次
 
學分
2.0 
全/半年
半年 
必/選修
選修 
上課時間
第1,4,5,6,7,9,10,11,16 週
星期一A,B(18:25~20:10) 
上課地點
生科4C 
備註
本課程以英語授課。密集課程。5/7.14.21.28、6/3-5實驗技術實作與微型研究計畫執行。與王佑群合開。
總人數上限:20人 
 
課程簡介影片
 
核心能力關聯
本課程尚未建立核心能力關連
課程大綱
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課程概述

Evolutionary developmental biology is an interdisciplinary field that merges developmental genetics with evolutionary biology to address questions concerning how changes at molecular level lead to morphological diversity. This course is designed to teach the general principles for students who are interested in this field. In addition, we will development innovative hands-on activities to allow students gain practical experiences. The main topic for this course is gastrulation.
Gastrulation is the first morphogenetic event of the developing animal embryo. In the metazoan embryo, the three germ layers – ectoderm, mesoderm and endoderm – are specified during gastrulation, as they undergo a distinct series of morphogenetic movements to differentiate and form lineage-specific tissue and organ structures. Drosophila melanogaster, one of the best known genetic model organisms, has been serving as a preeminent platform for the study of gastrulation morphogenesis. Not only have the studies in this species established a wealth of knowledge concerning cell fate patterning and tissue mechanics during gastrulation, they have also invited comparative studies using other insect species. Strikingly, gastrulation morphogenetic movements have been shown to substantially diverge within the lineage of Diptera, to which Drosophila belongs, raising the question of whether the divergences occurred incrementally or abruptly during the course of evolution. To address this question, we have conceived a research program that aims at a comparative analysis of gastrulation movement among the closely related Drosophila species. This program will seek a detailed sampling of representative species in the phylogenetic void between Drosophila melanogaster and the scuttle fly Megaselia abdita, the closest relative whose gastrulation has been previously examined. It will aim at identifying the conserved and diverged components of gastrulation morphogenesis within this phylogenetic spectrum and yielding a first look at the phylogenetic profile of gastrulation morphogenesis at the evolutionary timescale.
 

課程目標
Students will learn
1. General principle in evolutionary developmental biology,
2. General approaches in study evolutionary developmental biology questions,
3. Experimental design and imagine analyses of Drosophila embryogenesis, and
4. Literature reading, presentation and critical thinking.  
課程要求
General Biology, Genetics, Developmental Biology or equivalent 
預期每週課後學習時數
 
Office Hours
另約時間 備註: Please e-mail the instructors in advance. 
參考書目
The development of Drosophila melanogaster, edited by Michael Bate and Alfonso Martinez Arias, CSHL Press. (1993)
From DNA to diversity: Molecular genetics and the evolution of animal design by Sean B. Carroll, Jennifer K. Grenier, Scott D. Weatherbee, Wiley-Blackwell. (2004, 2nd Edition) 
指定閱讀
Martin, Adam C. “The Physical Mechanisms of Drosophila Gastrulation: Mesoderm and Endoderm Invagination.” Genetics 214, no. 3 (March 1, 2020): 543–60. https://doi.org/10.1534/genetics.119.301292.
Gheisari, Elham, Mostafa Aakhte, and H-Arno J Müller. “Gastrulation in Drosophila Melanogaster: Genetic Control, Cellular Basis and Biomechanics.” Mechanisms of Development 163 (September 1, 2020): 103629. https://doi.org/10.1016/j.mod.2020.103629.
Eritano, Anthony S, Claire L Bromley, Antonio Bolea Albero, Lucas Schütz, Fu-Lai Wen, Michiko Takeda, Takashi Fukaya, et al. “Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding.” Developmental Cell 53, no. 2 (April 20, 2020): 212-228.e12. https://doi.org/10.1016/j.devcel.2020.02.012. (For journal club)
Lemke, Steffen, Girish Kale, and Silvia Urbansky. “Comparing Gastrulation in Flies: Links between Cell Biology and the Evolution of Embryonic Morphogenesis.” Mechanisms of Development 164 (December 1, 2020): 103648. https://doi.org/10.1016/j.mod.2020.103648.
Wunderlich, Zeba, Charless C. Fowlkes, Kelly B. Eckenrode, Meghan D. J. Bragdon, Arash Abiri, and Angela H. DePace. “Quantitative Comparison of the Anterior-Posterior Patterning System in the Embryos of Five Drosophila Species.” G3: Genes, Genomes, Genetics 9, no. 7 (July 1, 2019): 2171–82. https://doi.org/10.1534/g3.118.200953. 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
  Introduction to evolutionary developmental biology (Chau-Ti Ting) 
第2週
  The genetic toolkit for development (Yu-Chiun Wang) 
第3週
  Evolution of the Toolkit (Chau-Ti Ting) 
第4週
  Diversity of body plans and body parts (Chau-Ti Ting) 
第5週
  Gastrulation as we know it in Drosophila: A D. melanogaster point-of-view (Yu-Chiun Wang) 
第6週
  Student Presentation: Gastrulation  
第7週
  Comparative gastrulation morphogenesis of insects (Steffen Lemke) 
第8週
  The phylogenetics of Drosophila and evolution of gene expression pattern in the early embryo (Chau-Ti Ting) 
第9週
  Lab. Drosophila biology and genetics
Lab. Embryo preparation and mounting 
第10週
  Lab. Injection of live dye or mRNA
Lab. Brightfield/DIC and confocal imaging 
第11週
  Student projects 
第11週
  Lab. Confocal imaging data analyses
Student projects 
第12週
  Student presentation