課程資訊
課程名稱
蛋白體與後修飾之質譜分析
Mass Spectrometry-based Proteomics and Protein Modification Analysis 
開課學期
103-1 
授課對象
生命科學院  生化科學研究所  
授課教師
邱繼輝 
課號
BChem5027 
課程識別碼
B46 U0840 
班次
 
學分
全/半年
半年 
必/選修
選修 
上課時間
星期二7,8(14:20~16:20) 
上課地點
 
備註
上課地點:中研院生化所409室。
總人數上限:30人 
 
課程簡介影片
 
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課程概述

THIS GRADUATE COURSE AIMS TO TEACH THE FUNDAMENTALS OF ADVANCED MASS SPECTROMETRY-BASED PROTEIN
MODIFICATIONS ANALYSIS AT BOTH SINGLE PROTEIN AND OMICS LEVEL. THE COURSE WILL BEGIN WITH AN
INTRODUCTION TO THE GENERAL ASPECTS OF BIOLOGICAL MASS SPECTROMETRY (MS), FOCUSING ON RECENT
ADVANCES IN MS INSTRUMENTATION AND TECHNIQUES, WHICH CONTRIBUTE TO CURRENT STATUS OF GLOBAL AND
TARGETED QUANTITATIVE SHOTGUN PROTEOMICS. THIS WILL THEN BE FOLLOWED BY EXTENDING THE TECHNICAL
PLATFORMS TO ANALYSIS OF A WIDE RANGE OF PROTEIN MODIFICATIONS, WITH AN EMPHASIS ON THE GENERAL
ASPECTS OF SAMPLE PREPARATION, ENRICHMENT, DATA ACQUISITION AND ANALYSIS. FINALLY, MORE IN DEPTH
DISCUSSION WILL BE GIVEN TO CURRENT TRENDS IN PROTEIN PHOSPHORYLATION AND GLYCOSYLATION ANALYSES,
ENCOMPASSING PHOSPHOPROTEOMICS, GLYCOPROTEOMICS AND GLYCOMICS, WITH PERSPECTIVES ON THEIR
RESPECTIVE BIOLOGICAL SIGNIFICANCE AND PRACTICAL IMPLICATIONS.
ALONG THESE LINES, THE COURSE CONTENT IS ORGANIZED INTO 3 MODULES :
MODULE I (4 LECTURES + 1 TUTORIAL SEMINAR)
- FUNDAMENTALS OF BIOLOGICAL MASS SPECTROMETRY
- RECENT ADVANCES IN INSTRUMENTATION AND TECHNIQUES
- ESSENTIALS OF QUANTITATIVE SHOTGUN PROTEOMICS
- CURRENT TRENDS AND FUTURE DIRECTIONS
MODULE 2 (4 LECTURES + 1 TUTORIAL SEMINAR)
- DIVERSITY IN PROTEIN CHEMICAL AND POST-TRANSLATIONAL MODIFICATIONS
- PRACTICAL CONSIDERATIONS IN MS-BASED PROTEIN MODIFICATION ANALYSIS
- PROTEIN PHOSPHORYLATION ANALYSIS AND QUANTITATIVE PHOSPHOPROTEOMICS
- THE BIOLOGY AND THE OUTSTANDING ISSUES
MODULE 3 (4 LECTURES + 1 TUTORIAL SEMINARS)
- ESSENTIALS OF PROTEIN GLYCOSYLATION AND GLYCOBIOLOGY FROM STRUCTURAL ANALYSIS PERSPECTIVES
- WORKFLOWS IN SINGLE GLYCOPROTEIN ANALYSIS, TOWARDS MAPPING SITE-SPECIFIC GLYCOSYLATION PATTERN
- MS-BASED GLYCOMICS AND GLYCOPROTEOMICS
- REALISTIC (AND IMPORTANT) GOALS VERSUS THE GRAND (AND UNREALISTIC) VIEW

EACH MODULE WILL COMMENCE WITH 3-4 LECTURES COVERING THE CONTENT AS OUTLINED ABOVE. THESE WILL BE
FOLLOWED BY 1-2 TUTORIAL/SEMINAR TO REVIEW THE REPORTED FINDINGS, 

課程目標
 
課程要求
ALTHOUGH DESIGNATED AS A PH.D. COURSE AND SUITS BEST STUDENTS ALREADY WITH WORKING EXPERIENCE ON
MS-BASED PROTEIN ID, IT IS OPEN TO ALL INTERESTED GRADUATES AND UNDERGRADUATES WITH REFRESHING MINDS
AND ENTHUSIASM. NO PRE-REQUISITE IS STIPULATED BUT A GOOD BACKGROUND KNOWLEDGE ON PROTEINS AND
ANALYTICAL CHEMISTRY WILL BE HIGHLY DESIRABLE. 
預期每週課後學習時數
 
Office Hours
 
參考書目
 
指定閱讀
 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
無資料