課程資訊
課程名稱
蛋白體與後修飾之質譜分析
Mass Spectrometry-based Proteomics and Protein Modification Analysis 
開課學期
106-2 
授課對象
生命科學院  生化科學研究所  
授課教師
邱繼輝 
課號
BChem5027 
課程識別碼
B46 U0840 
班次
 
學分
2.0 
全/半年
半年 
必/選修
選修 
上課時間
 
上課地點
 
備註
本課程中文授課,使用英文教科書。地點為中研院生化所409室,時間:週一下午4點~6點。外籍生請明年再行修課,明年為英文授課
總人數上限:30人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1062BChem5027_ 
課程簡介影片
 
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課程概述

106(2)為中文上課,請外籍學生於107(2)再行修課。
The course this semester will be taught in Chinese, foreign students please take this course next year

This course aims to teach the fundamentals of biological mass spectrometry with particular focus on applications to proteomics and analysis of protein modifications. The course will begin with an introduction to the general aspects of mass spectrometry-based peptide sequencing and protein identification, along with recent technological developments in the context of advancing quantitative shotgun proteomic analysis. Current perspectives in global versus directed analysis, speed versus depth in proteomic coverage, will be discussed. This will then be followed by introducing the practical aspects of MS analysis of select protein modifications, at both the levels of single targeted protein of interest and the proteomic scale. Topics include phosphoproteomics; lysine modifications such as acetylation, methylation, ubiquitination, and SUMOylation; cysteine modifications such as oxidation, glutathionylation and S-nitrosylation; and glycosylation. The last further accompanied by an overview of MS-based glycomics and glycoproteomics. 

課程目標
待補 
課程要求
Opens to life science Master and PhD Students, as well as keen senior undergraduates major in Biochemistry, who have a basic knowledge of protein chemistry and structures, and the commonly found modifications. No absolute prerequisite is set but will be advantageous and encouraged to have first taken one or more of the core courses offered at 生化所 in the first semester eg 蛋白質修飾機制與細胞訊息;結構生化學. Chemistry graduates interested in biology and major in chemical biology are also encouraged and accepted. 
預期每週課後學習時數
 
Office Hours
 
參考書目
待補 
指定閱讀
待補 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
2/26  因教師出國,停課一次
no class 
第2週
3/5  Overview on DDA & Technical Evolu;on of MS-based Proteomics I 
第3週
3/12  DDA & Technical Evolu;on of MS-based Proteomics II 
第4週
3/19  Quan;ta;ve Shotgun Proteomics 
第5週
3/26  From Global Discovery to Targeted Proteomics, SRM/PRM & DIA 
第6週
4/2  溫書假 Holiday, no class, reading and preparing for seminars 
第7週
4/9  Quantitative Proteomics to probe Spaiotemporal Proteome Dynamics 
第8週
4/16  Proteome Network, Complexes & Interactomics 
第9週
4/23  Proteomics Seminars I 
第10週
4/30  Proteomics Seminars II 
第11週
5/7  PTM - General Technical Considera;ons; Phospho 
第12週
5/14  No class, reading and preparing for seminars 
第13週
5/21  PTM - Lys Modifica;ons : Ub/SUMO, Ac/Me 
第14週
5/28  PTM - Glycosyla;on 
第15週
6/4  PTM - Seminars I 
第16週
6/11  PTM - Seminars II 
第17週
6/18  端午節 Holiday, no class 
第18週
6/25  Final Recap with Q&A Oral Exam