|
週次 |
日期 |
單元主題 |
|
第1週 |
9/17 |
課程介紹/1. Plasmid expression, Site-directed mutagenesis (Science 2013: A secreted PTEN phosphatase that enters cells to alter signaling and survival) |
|
第2週 |
9/24 |
2. Quantitative PCR, EMSA, Reporter assay, Cell growth assay (PNAS 2012: The polymorphism rs944289 predisposes to papillary thyroid carcinoma through a large intergenic noncoding RNA gene of tumor suppressor type) |
|
第3週 |
10/01 |
3. Gene knockout mouse, Conditional knockout (PNAS 2013: Shox2 is a molecular determinant of depot-specific adipocyte function) |
|
第4週 |
10/08 |
4. ChIP, DNA pull-down, IP/Co-IP (PNAS 2013: RARα-PLZF oncogene inhibits C/EBPα function in myeloid cells) |
|
第5週 |
10/15 |
不上課 |
|
第6週 |
10/22 |
5. CLIP, In vitro transcription, siRNA/shRNA, MTT, Colony formation, Xenograft (Nat Struct Mol Biol 2012: Intronic RNAs mediate EZH2 regulation of epigenetic targets) |
|
第7週 |
10/29 |
6. Tandem affinity purification, Mass analysis, SILAC (PNAS 2010: ALS-associated mutations in TDP-43 increase its stability and promote TDP-43 complexes with FUS/TLS) |
|
第8週 |
11/05 |
7. Viral expression systems, Proximity ligation assay (PNAS 2013: Transcription factor YY1 is essential for regulation of the Th2 cytokine locus and for Th2 cell differentiation) |
|
第9週 |
11/12 |
8. Artificial Zinc finger, Protein expression in E. coli (PNAS 2012: Neutralizing the function of a β-globin–associated cis-regulatory DNA element using an artificial zinc finger DNA-binding domain) |
|
第10週 |
11/19 |
不上課 |
|
第11週 |
11/26 |
期末考 |