課程資訊
課程名稱
分子選殖與重組蛋白的表達與純化的技術與實
Practical Course on Recombinant Protein Production and Purification 
開課學期
108-1 
授課對象
生命科學院  生化科學研究所  
授課教師
 
課號
BChem5041 
課程識別碼
B46 U1010 
班次
 
學分
2.0 
全/半年
半年 
必/選修
選修 
上課時間
 
上課地點
 
備註
開學第一週上課時間另行通知。須向授課老師取得授權碼方得上網選課。
總人數上限:6人 
 
課程簡介影片
 
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課程概述

The recombinant protein expression and purification is one of useful technologies in modern biology. Many experiments requires pure recombinant protein. In addition, having recombinant protein in the native and function states is critical to have good experimental outcomes. In this class, I will introduce some basis knowledge of molecular cloning and recombinant protein expression and purification. By hand-on experiments, students will acquire the protein expression and purification skills 

課程目標
1. Understanding modern technology of molecular cloning
2. Knowing basis of recombinant protein expression and purification
3. Acquiring protein expression and purification skills 
課程要求
待補 
預期每週課後學習時數
 
Office Hours
 
指定閱讀
 
參考書目
1. High Throughput Protein Expression and Purification Volume 498 of the series Methods in Molecular Biology pp 105-115
2. Recombinant Protein Purification Handbook - Principles and Methods
3. Enzymatic assembly of overlapping DNA fragments by Gibson DG. 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
無資料