生物技術核心實驗課程
(一)、課程大綱：
本課程為生物技術學程之重要基礎實驗科目，整個流程以分子生物中心法則(Central Dogma)為骨幹，學習由GUS酵素基因之DNA開始，經過剪接與基因轉殖，從宿主細胞中表現並檢定其mRNA，然後收集純化並檢定目標酵素。本課程由基本的自動吸管開始，教導基礎的核酸及蛋白質操作技術，對出入生物技術領域的初學者有很大的幫助。
1. DNA manipulation
(1). Basic Techniques
a. Pipetting
b. Spectrometry
c. Bacterial culture techniques
(2). DNA preparation
a. Miniprep of plasmid DNA
b. Restriction analysis
c. Large scale digestion of plasmid
(3). DNA ligation
a. Isolation of DNA fragment
b. Quantification analysis of DNA
c. DNA ligation
(4). Transformation
a. Transformation by CaCl2
(5). Protein expression system
a. Colony hybridization
b. Histochemical detection
(6). Detection of specific nucleic acid sequences by hybridization
a. mini-prep of plasmid DNA
b. Restriction analysis
c. Southern boltting

2. RNA hybridization
(1). RNA Preparation
a. Total RNA isolation
b. In vitro transcription
(2). DIG-labeled probe preparation
a. Random priming
b. PCR reaction
(3). Northern blot hybridization
a. RNA analysis
b. Northern blotting
c. Northern blot hybridization
d. Detection of probe by enzyme immunoassay
e. Analysis of the blot

3. Protein analysis
(1). Protein purification
a. Protein extraction
b. Gel filtration
(2). Affinity chromatography
a. Affinity chromatography
b. Activity assay
(3). Electrophoresis analysis
a. SDS-PAGE
b. Protein transfer
c. Immunostaining