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課程名稱 |
生物技術核心實驗
Biotechnology Core Techniques |
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開課學期 |
103-1 |
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授課對象 |
生物技術學程 |
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授課教師 |
杜宜殷 |
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課號 |
Prog5074 |
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課程識別碼 |
P05 U2020 |
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班次 |
02 |
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學分 |
4 |
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全/半年 |
半年 |
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必/選修 |
必修 |
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上課時間 |
星期三2,3,4,@,5,6,7,8(9:10~16:20) |
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上課地點 |
生技館213 |
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備註 |
初選不開放。請於8/18-8/28洽生技中心報名。需繳學分費。與徐駿森、陳仁治合開
總人數上限：40人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1031Prog5074_02 |
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課程簡介影片 |
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核心能力關聯 |
本課程尚未建立核心能力關連 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
Students will be asked to choose a topic on horticulture, collect, read, and organize literatures, write up abstract, oral present with media aid, control report time, ask and answer questions, write up report, and service. |
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課程目標 |
This course will train senior students to know the research trend of the world. Students are asked to collect and organize references, dig out questions, innovate ideas, write a report, oral present, answer questions properly, and obey to the rules. These abilities are required for both advanced study and job working. |
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課程要求 |
Present an oral and written report on horticulture related topics and discuss in classes. |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
待補 |
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參考書目 |
Journals and books related on horticulture, plant science, food processing,
and biotechnology. |
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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第2週 |
2014/09/24 |
1. Introduction 2. Cloning tools |
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第3週 |
2014/10/01 |
Cloning strategies |
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第4週 |
2014/10/08 |
Isolation and quantitative analysis of DNA |
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第5週 |
2014/10/15 |
Transformation and identification of transformants |
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第6週 |
2014/10/22 |
Protein expression systems |
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第7週 |
2014/10/29 |
Detection of specific nucleic acid sequences by hybridization |
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第8週 |
2014/11/05 |
1. RNA in general
2. RNA preparation
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第9週 |
2014/11/12 |
Denaturing gel electrophoresis |
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第10週 |
2014/11/19 |
1.Probe preparation
2. Northern hybridization analysis
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第11週 |
2014/11/26 |
Analysis of RNA by primer extension, S1 analysis, RNase protection and nuclear run-off |
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第12週 |
2014/12/03 |
Protein in general
Protein extraction
Gel filtration chromatography
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第13週 |
2014/12/10 |
Ion exchange chromatography
Affinity chromatography
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第14週 |
2014/12/17 |
Protein assay
Polyacrylamide electrophoresis
protein transfer
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第15週 |
2014/12/24 |
Immunological tools |
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第16週 |
2014/12/31 |
Protein Examination |
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