Course Information
Course title
Molecular Biology 
Semester
101-1 
Designated for
VARIOUS PROGRAM  MOLECULAR MEDICINE PROGRAM  
Instructor
LIN, JING-JER 
Curriculum Number
md&ph5001 
Curriculum Identity Number
420 U0100 
Class
 
Credits
Full/Half
Yr.
Half 
Required/
Elective
Required 
Time
Tuesday 8,9 Thursday 8,9 
Remarks
The upper limit of the number of students: 200. 
Ceiba Web Server
http://ceiba.ntu.edu.tw/1011molbio 
Table of Core Capabilities and Curriculum Planning
Association has not been established
Course Syllabus
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Course Description

見醫衛共同課程時間表 

Course Objective
讓研究基礎醫學的研究生,瞭解原核生物與真核細胞內,核酸與蛋白質
等巨分子的結構與功能,以奠定其醫學研究的基礎。
 
Course Requirement
 
Office Hours
 
References
 
Designated reading
 
Grading
   
Progress
Week
Date
Topic
第1週
9/11,9/13  (I)Introduction (II)DNA structure and function (1)DNA as store of information (A) Outline of major experiments that led to the acceptance of DNA as the genetic material (B) Structure of nucleic acid, base pairing, tautomeric shift, DNA-A,-B,-Z forms (C) Denaturing and renaturing of DNA (D) Supercoiled DNA and topoisomerase 
第2週
9/18,9/20  (2) DNA technology, genomic size and genetic content (3) Genome: organization and evolution 
第3週
9/25,9/27  (4) Extrachromosome DNA, nucleosome, chromosome and chromatid (5) DNA replication (A) Prokaryotic system 
第4週
10/02,10/04  (B) Eukaryotic system (C) Telomere (6) The dynamic genome (A) Recombination and transposition of DNA in the prokaryotic genome (B) Rearrangement and amplification in the eukaryotic genome 
第5週
10/09,10/11  (7)DNA damage and mutation (8)DNA repair and modification 
第6週
10/16,10/18  第一次期中考 (考試範圍 9/11~10/11) (III)Transcription, regulation and processing (1) Prokaryotic Transcription and Eukaryotic Pol II Transcription 
第7週
10/23,10/25  (2) Transcription and Epigenetic Regulation (3) Eukaryotic transcription and regulation (eukaryotic RNA pol. I and III) 
第8週
10/30,11/01  (4) Post-transcriptional processing, ribozyme and regulation (IV)Translation (1) Assembly line for protein synthesis (2) Transfer RNA is the translated adaptor 
第9週
11/06,11/08  (3) Ribosomes provide a translation factory (4) Codons and their significance (5) Translation in cell free system (6)未確定 
第10週
11/13,11/15  (7) Post-translational modification I. Structure II.Protein ubiquitination (V)Recombinant DNA technology(I)  
第11週
11/20,11/22  (1) Cloning of DNA:transformation and screening of transformants (2) Construction of cDNA and genomic libraries (3) Colony and plaque hybridization, Southern blot, Northern blot, Western blot analysis (4) Primer extension, S1 nuclease mapping and RNase protection assay (5) Differential display (6) Site-directed mutagenesis and polymerase chain reaction (7) Application of restriction fragment length polymorphism and variable number tandem repeat for genetic disease analysis (8) Genome-wide sequencing strategies 
第12週
11/27,11/29  (9) Phage strategies : control of lytic and lysogenic pathway (10) Phage display library (11) A brief introduction to animal viruses 第二次期中考(考試範圍 10/18~11/22) (VI)Construction of the cells 
第13週
12/04,12/06  (1) Signal transduction in prokaryotes (2) Signal transduction in eukaryotes 張富雄 (VII)Genes in the development (1) Immune diversity and immunotherapy 
第14週
12/11,12/13  (2) Gene regulation and development (3) Oncogenes (4) Tumor suppressor genes 
第15週
12/18,12/20  (VIII)Recombinant DNA technology(II) (1) Expression of cloned gene in prokaryotic and eukaryotic cells (2) Yeast genetics and its applications (3) Applications of bioinformatics and proteomics in biomedical research 
第16週
12/25,12/27  (4) Current development of genome projects and their impacts to human diseases (5) Transgenic techniques 
第17週
1/01,1/03  (6) Gene therapy (A) Viral vectors (B) Clinical Application 期末考 (考試範圍 12/04~01/03)